Article
Optics
Mingyin Yao, Gangrong Fu, Jiang Xu, Tao Li, Linying Zhang, Muhua Liu, Ping Yang, Yuan Xu, Honghui Rao
Summary: The study used LIBS technology to classify citrus huanglongbing, achieving 100% classification accuracy by analyzing the physical characteristics and chemical composition of the fruit.
Article
Plant Sciences
Renato Beozzo Bassanezi, Isabela Vescove Primiano
Summary: This study compared the management of Huanglongbing and citrus variegated chlorosis during different time periods, showing that vector control and inoculum removal at different seasons did not significantly impact the progression of the diseases.
Article
Plant Sciences
Richard D. Lally, Kathleen Donaleshen, Ulalo Chirwa, Katie Eastridge, Wesley Saintilnord, Edward Dickinson, Richard Murphy, Steven Borst, Karina Horgan, Karl Dawson
Summary: This study demonstrates that microbial fermentation application (MFA) increases copper concentration in citrus trees, stabilizes the disease index (DI) in infected trees, and induces the expression of PR2 and PAL genes, enhancing defense responses against Huanglongbing (HLB).
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Agronomy
Minli Bao, Zheng Zheng, Jianchi Chen, Xiaoling Deng
Summary: Huanglongbing (HLB) is a devastating disease affecting citrus production worldwide, and in China it is associated with the unculturable alpha-proteobacterium Candidatus Liberibacter asiaticus (CLas). Phages/prophages of CLas have been identified through genomic research, aiding in understanding CLas biology. This study investigated the symptom development of different citrus cultivars infected with CLas strains carrying Type 1 and Type 2 phages, providing baseline information for future research on the roles of CLas phages in HLB symptom development.
Review
Plant Sciences
Samuel Kwakye, Davie M. M. Kadyampakeni
Summary: Enhanced nutritional programs (ENPs) have improved the growth and development of citrus trees affected by Huanglongbing (HLB) by increasing the rates of manganese (Mn) and iron (Fe) application. Maintaining optimal levels of these micronutrients is critical in overcoming deficiency symptoms and allowing for photosynthetic activities and physiological processes in the citrus trees. Manipulating micronutrient availability through fertilizer application is an important mitigation strategy for HLB-affected citrus trees in Florida.
Article
Agriculture, Multidisciplinary
Jingwen Li, Fred G. Gmitter Jr, Boce Zhang, Yu Wang
Summary: This study utilizes an integrated analysis of untargeted metabolomics and microbiomics data to explore the interactions between plant metabolism and plant-associated bacteria in the development of HLB. Machine learning models are applied to identify important metabolites and bacteria and provide potential solutions for controlling HLB.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Biotechnology & Applied Microbiology
Joana R. M. Ferreira, Isabel N. Sierra-Garcia, Samuel Guieu, Artur M. S. Silva, Raquel Nunes da Silva, Angela Cunha
Summary: Citrus, as economically important fruit crops, suffer significant losses from infectious diseases. Traditional control measures involve chemical treatment and eradication of infected plants, while innovative antimicrobial approaches like photodynamic inactivation are being explored. However, the widespread application of photodynamic inactivation in controlling citrus diseases requires consideration of various microorganisms.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Microbiology
Marcelo L. Merli, Kaylie A. Padgett-Pagliai, Alexandra E. Cuaycal, Lucila Garcia, Maria Rosa Marano, Graciela L. Lorca, Claudio F. Gonzalez
Summary: The study revealed that a potential effector LotP of 'Candidatus Liberibacter asiaticus' may interact with chaperones like GroEL and Hsp40 to enhance Lon protease activity, leading to damage in citrus plant tissues. LotP may also interact with plant proteins, indicating its potential role in protease activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Clebson S. Tavares, Ruchir Mishra, Pierre N. Ghobrial, Bryony C. Bonning
Summary: In this study, the authors compared the gut surface proteins of adult and nymph Asian citrus psyllid and identified proteins that are potentially exploited by the pathogen Candidatus Liberibacter asiaticus (CLas) during transmission. The findings provide insights into the interaction between CLas and the psyllid gut and offer potential targets for disrupting this interaction.
JOURNAL OF PROTEOMICS
(2022)
Article
Plant Sciences
Jiao Du, Qiying Wang, Chunhua Zeng, Changyong Zhou, Xuefeng Wang
Summary: A newly discovered secretory protein, AGH17470, associated with Huanglongbing, has been found to trigger plant immune response, promote growth and development, and enhance resistance to pathogens.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Plant Sciences
Joon Hyuk Suh, Anirban Guha, Zhixin Wang, Sheng-Yang Li, Nabil Killiny, Christopher Vincent, Yu Wang
Summary: This study demonstrates that shading can alleviate the detrimental effects of Huanglongbing (HLB) on citrus trees. Through metabolomics and physiological approaches, the underlying metabolic mechanisms related to shade-induced morpho-physiological changes in citrus were investigated. Moderate shading prevents excessive starch accumulation, increases foliar sucrose levels, and promotes plant growth and fruit yield.
Article
Plant Sciences
Pan Shen, Xueyi Li, Shimin Fu, Changyong Zhou, Xuefeng Wang
Summary: This study found that a peptide called SECP8 secreted by the citrus huanglongbing bacterium CLas has the ability to suppress immune responses and promote disease development. It also affects the expression of immune-related genes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Joon Hyuk Suh, Xixuan Tang, Yi Zhang, Frederick G. Gmitter, Yu Wang
Summary: This study used a multiple pathway-targeted metabolomic approach to elucidate the metabolic mechanisms of HLB tolerance in citrus. The findings revealed a pivotal metabolic network associated with HLB tolerance, showing that pathways linked to plant growth, phloem regeneration, and energy-yielding metabolism were upregulated in HLB-tolerant cultivars. Additionally, metabolites related to plant defense responses were decreased in the tolerant group, indicating a strategy of sustaining plant growth and phloem formation rather than direct plant defense against the disease.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Microbiology
Orquidea Perez-Gonzalez, Ricardo Gomez-Flores, Patricia Tamez-Guerra
Summary: This review summarizes the studies on Hirsutella citriformis Speare, with a focus on its potential as a control agent for citrus crop pests and the Asian citrus psyllid, which causes the Huanglongbing disease. The review highlights the limited characterization of H. citriformis strains and the parasitization efficacy under suitable environmental conditions. However, there is still limited understanding of its mode of action and biocontrol potential.
Article
Mathematics, Applied
Pushpendra Kumar, Vedat Suat Erturk, Kottakkaran Sooppy Nisar
Summary: The study utilized a fractional huanglongbing model to analyze the transmission dynamics of citrus greening disease, solving the model with Caputo and a new generalized form of Caputo type fractional derivatives. Graphical observations with real numerical data were used to show the nature of the model classes, and the analysis of solution existence was supported by theorems. The numerical techniques were found to be strong and effective in showing the dynamics of the model over time.
MATHEMATICAL METHODS IN THE APPLIED SCIENCES
(2021)
Article
Biochemical Research Methods
Loraine Carolina Goenaga-Mafud, Yordania Matos Gamez, Carolina P. Campos, Jose Dirceu Vollet-Filho, Natalia Mayumi Inada, Cristina Kurachi, Vanderlei Salvador Bagnato
Summary: This article demonstrates the feasibility of reducing bacterial load in a kidney model by using ultraviolet-C as a germicidal agent. The results show that the bacterial load in the perfusate can be fully eliminated in less than 30 minutes of liquid circulation and irradiation. A modeling approach is used to verify the decrease in bacterial load when an organ is present in the circuit.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Cesar Augusto Abreu-Pereira, Marlise Inez Klein, Carmelia Isabel Vitorino Lobo, Ana Luiza Gorayb Pereira, Claudia Carolina Jordao, Ana Claudia Pavarina
Summary: This study evaluated the effectiveness of DNase I combined with antimicrobial photodynamic therapy against biofilms formed by a fluconazole-resistant Candida albicans strain. The results showed that DNase treatment combined with aPDT could significantly reduce cell viability and the components of the biofilm.
Article
Chemistry, Organic
Aline A. N. de Souza, Aloisio de A. Bartolomeu, Timothy J. Brocksom, Timothy Noel, Kleber T. de Oliveira
Summary: The electrochemical sulfenylation reaction was investigated in both batch and continuous flow regimes, with quaternary ammonium salts identified as the best mediators. The tolerance of Boc-cysteine to the reaction protocol was noted, increasing its relevance. The methodology was found to be scalable in both batch and continuous flow conditions, offering potential for further studies involving important functional groups in organic synthesis.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Cardiac & Cardiovascular Systems
Cynthia Aparecida Castro, Tereza Cristina Buzinari, Rafael Luis Bressani Lino, Heloisa Sobreiro Selistre de Araujo, Fernanda de Freitas Anibal, Roberto Mario Machado Verzola, Vanderlei Salvador Bagnato, Natalia Mayumi Inada, Gerson Jhonatan Rodrigues
Summary: The study evaluated the role of TNF-α and IL-6 in foam cell formation, confirming that higher concentrations of FITC-ox-LDL led to more accumulation of lipoproteins in cells and higher production of TNF-α and IL-6.
ARQUIVOS BRASILEIROS DE CARDIOLOGIA
(2022)
Article
Entomology
Izabela N. Nascimento, Mirian F. F. Michereff, Walter E. Pereira, Paulino R. Villas-Boas, Marcos Rafael Gusmao, John Caufield, Raul A. Laumann, Miguel Borges, Maria Carolina Blassioli-Moraes
Summary: Plants respond to herbivore injury by emitting specific volatiles that attract the enemies of herbivores. This study evaluated whether maize plants can recognize damage from a stink bug and found that while the stink bug does not use plant volatiles as host location cues, feeding by the stink bug activates maize indirect defense, inducing emission of attractant volatiles for the herbivore's enemy.
ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA
(2023)
Article
Biotechnology & Applied Microbiology
Matheus Luis Docema, Tatiana de Souza Moraes, Lisia Borges Attilio, Joao Paulo Rodrigues Marques, Liliane Cristina Liborio Stipp, Ricardo Harakava, Juliana Freitas-Astua, Jose Belasque Junior, Ricardo Antunes Azevedo, Salete Aparecida Gaziola, Francisco de Assis Alves Mourao Filho
Summary: Currently, there are no resistant sweet orange cultivars to citrus canker. Transgenic plants expressing antimicrobial peptides and resistance genes from tolerant cultivars were evaluated for resistance to Xanthomonas citri subsp. citri (Xcc). Transgenic plants showed reduced incidence and severity of citrus canker, as well as lower Xcc titers. Higher superoxide dismutase activity in transgenic plants led to increased hydrogen peroxide production. Callose deposition, an important plant defense response, was observed in transgenic plants with lower Xcc populations.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Review
Plant Sciences
Pedro Luis Ramos-Gonzalez, Gabriella Dias Arena, Aline Daniele Tassi, Camila Chabi-Jesus, Elliot Watanabe Kitajima, Juliana Freitas-Astua
Summary: Kitaviridae is a family of plant-infecting viruses that have unique localized infections and show deficient movement. They are transmitted by mites and infect a wide range of host plants, causing economic damage to crops.
ANNUAL REVIEW OF PHYTOPATHOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Silvia de Oliveira Dorta, Lisia Borges Attilio, Odimar Zanuzo Zanardi, Joao Roberto Spotti Lopes, Marcos Antonio Machado, Juliana Freitas-Astua
Summary: The Asian citrus psyllid (ACP) is the vector of the devastating disease huanglongbing (HLB) in citrus. Previous research has shown the potential of using Bt strains containing cry/cyt genes to control ACP nymphs. This study attempted to produce transgenic sweet orange plants expressing the cry11A gene from Bt, and confirmed the presence and copy number of the transgene using PCR and Southern blot analyses. Greenhouse mortality assays showed that the tested events had effects ranging from 22% to 43% on D. citri nymphs after a five-day exposure period. This is the first report of citrus plants expressing the Bt cry11A gene for the management of D. citri nymphs.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Camila Chabi-Jesus, Pedro Luis Ramos-Gonzalez, Aline Daniele Tassi, Laura Rossetto Pereira, Marines Bastianel, Douglas Lau, Maria Cristina Canale, Ricardo Harakava, Valdenice Moreira Novelli, Elliot Watanabe Kitajima, Juliana Freitas-Astua
Summary: Citrus leprosis (CL) is a viral disease that seriously affects Brazilian citriculture. In this study, a new virus called citrus bright spot virus (CiBSV) was discovered in sweet orange trees affected by CL in small orchards in Southern Brazil. Sequence analysis revealed that CiBSV belongs to the genus Dichorhavirus and clusters with citrus leprosis virus N, which is transmitted by Brevipalpus phoenicis. Brevipalpus papayensis and B. azores were found in CiBSV-infected citrus plants, with B. azores being the only vector capable of transmitting the virus to Arabidopsis plants.
Article
Agricultural Engineering
Gabriel da Silva Amaral, Ricardo Alcantara-de la Cruz, Rodrigo Martinelli, Luiz Renato Rufino Junior, Leonardo Bianco de Carvalho, Fernando Alves de Azevedo, Maria Fatima das Gracas Fernandes da Silva
Summary: Glyphosate resistance was found in multiple weed species in citrus orchards in São Paulo, Brazil, and multiple resistant species were present within the same orchard.
Article
Chemistry, Analytical
Luis Carlos Leva Borduchi, Carlos Renato Menegatti, Debora Marcondes Bastos Pereira Milori, Helcio Jose Izario Filho, Paulino Ribeiro Villas-Boas
Summary: In this study, calibration-free LIBS with the one-point calibration method was used to reduce matrix effects in the quantification of mercury in soil and landfill leachate samples. By optimizing the LIBS system parameters for each sample matrix and applying the OPC factors, an average error of 4.3% was achieved in the quantification of Hg.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2023)
Article
Biochemistry & Molecular Biology
Jose Diogo E. Reis, Paulo Wender P. Gomes, Paulo R. da C. Sa, Sonia das G. S. R. Pamplona, Consuelo Yumiko Y. e Silva, Maria Fatima das G. F. da Silva, Anupam Bishayee, Milton Nascimento da Silva
Summary: This study identified new potential mass spectrometry signatures for phragmalin-type limonoids in Swietenia macrophylla leaves, providing valuable information for future drug development efforts.
Article
Chemistry, Multidisciplinary
Karen Susana M. Madera, Luis Guillermo C. Durango, Orfa Ines C. Martinez, Maria Fatima G. F. da Silva, Joao B. Fernandes, Moacir R. Forim
Summary: This study aimed to determine the relationship between the intestinal microbiota of the cucurbit beetle and its ability to degrade antibiotics. The results revealed that bacterial strains isolated from the gastrointestinal tract of the beetles showed a high degradation rate of doxycycline in media supplemented with the antibiotic.
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2023)
Article
Plant Sciences
Gabriella D. Arena, Pedro L. Ramos-Gonzalez, Aline D. Tassi, Marcos A. Machado, Juliana Freitas-Astua
Summary: This study developed two TaqMan RT-qPCR assays for detecting and quantifying Citrus leprosis virus C (CiLV-C) lineage SJP, which is prevalent in the Brazilian citrus belt and the world's main sweet orange production area. The assays targeted two specific genes, p29 and RdRp, and were proven to be efficient and sensitive. By quantifying the target genes in different tissues and organisms, the study revealed the distribution and accumulation of CiLV-C molecules, the higher quantities in sweet orange fruits compared to stems and leaves, as well as the progressive increase during the early stages of infection. These findings contribute to a better understanding of the epidemiology and virus-vector interaction of citrus leprosis.
TROPICAL PLANT PATHOLOGY
(2023)
Article
Optics
N. Piovella, G. R. M. Robb, R. Bachelard
Summary: This study demonstrates the coherent transfer of orbital angular momentum (OAM) from a laser beam to a Bose-Einstein condensate in a ring trap, in quantized units of h??. The control of specific angular momentum states allows for the generation of light with different OAM than the pump light through the imprinting of the atomic ring on the scattered light.
Article
Agricultural Engineering
Yifan Bai, Junzhen Yu, Shuqin Yang, Jifeng Ning
Summary: A real-time recognition algorithm (Improved YOLO) is proposed in this paper for accurately identifying small, similar-colored, and overlapping strawberry seedling flowers and fruits. The experimental results show that the algorithm achieves high precision, recall, and average precision, and meets the real-time detection requirements, providing effective support for the automated management of strawberry seedling flower and fruit thinning.
BIOSYSTEMS ENGINEERING
(2024)
