Journal
BIOSENSORS & BIOELECTRONICS
Volume 80, Issue -, Pages 352-358Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.01.069
Keywords
Electrochemiluminescence immunosensor; EuPO4 nanowire; Prostate specific antigen
Categories
Funding
- National Natural Science Foundation of China [21175057, 21375047, 21377046, 21575050, 21505051]
- Science and Technology Plan Project of Jinan [201307010]
- Science and Technology Development Plan of Shandong Province [2014GSF120004]
- UJN [ts20130937]
- Special Foundation for Taishan Scholar Professorship of Shandong Province
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EuPO4 nanowire, which exhibited strong and stable cathodic electrochemiluminescence (ECL) activity, was used for the first time to fabricate an immunosensor for the detection of prostate specific antigen (PSA). EuPO4 has some inherent excellent properties such as long luminescence lifetime, narrow emission band, high quantum yield and low toxicity. Based on these properties, a novel label-free ECL immunosensor was developed using EuPO4 as a sensing matrix. Chitosan solution was used to disperse EuPO4 nanowires and the amino groups on chitosan enabled the covalent attachment of capture antibodies. After the modification of the electrode surface with EuPO4 nanowires, anti-PSA was then immobilized on them, forming a label-free immunosensing interface. The specific binding of PSA on the electrode inhibited the ECL reaction of EuPO4 nanowires with the coreactant due to the steric hindrance effect (Deng et al., 2013). Under the optimum conditions, a good linear relationship between ECL intensity and the logarithm of PSA concentration was obtained in the range of 0.0005-80 ng/mL with a detection limit of 177.33 fg/mL. The proposed ECL immunosensor showed good stability, acceptable selectivity and reproducibility. (C) 2016 Elsevier B.V. All rights reserved.
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