Journal
BIOSENSORS & BIOELECTRONICS
Volume 78, Issue -, Pages 140-146Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2015.11.039
Keywords
SNP typing; Cancer prognosis; Rotating chip; ligation-RCA; TP53
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Funding
- Engineering Research Center of Excellence Program of Korea Ministry of Science
- ICT Future Planning (MSIP)/National Research Foundation of Korea (NRF) [2014R1A5A1009799]
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Genetic variations such as single nucleotide polymorphism (SNP) and point mutations are important biomarkers to monitor disease prognosis and diagnosis. In this study, we developed a novel rotary microfluidic device which can perform multiplex SNP typing on the mutation sites of TP53 genes. The microdevice consists of three glass layers: a channel wafer, a Ti/Pt electrode-patterned resistance temperature detector (RTD) wafer, and a rotary plate in which twelve reaction chambers were fabricated. A series of sample injection, ligation-rolling circle amplification (L-RCA) reaction, and fluorescence detection of the resultant amplicons could be executed by rotating the top rotary plate, identifying five mutation points related with cancer prognosis. The use of the rotary plate eliminates the necessity of microvalves and micropumps to control the microfluidic flow in the channel, simplifying the chip design and chip operation for multiplex SNP detection. The proposed microdevice provides an advanced genetic analysis platform in terms of multiplexity, simplicity, and portability in the fields of biomedical diagnostics. (C) 2015 Elsevier B.V. All rights reserved.
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