Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 687, Issue -, Pages 839-848Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2019.06.102
Keywords
Rat; Brain; Proteomics; Particulate matter; Nano pollution
Categories
Funding
- PROTEOMASS Scientific Society
- FCT 2015 Investigator Program [IF/00007/2015]
- Associate Laboratory for Green Chemistry-LAQV - national funds from FCT/MCTES [UID/QUI/50006/2019]
- Portuguese Foundation for Science and Technology [SFRH/BD/109201/2015, SFRH/BD/120537/2016]
- Health Effects of Air Pollution Foundation [PBOC-5]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/109201/2015, SFRH/BD/120537/2016] Funding Source: FCT
Ask authors/readers for more resources
The adverse effects of air pollution have been long studied in the lung and respiratory systems, but the molecular changes that this causes at the central nervous system level have yet to be fully investigated and understood. To explore the evolution with time of protein expression levels in the brain of rats exposed to particulate matter of different sizes, we carried out two-dimensional gel electrophoresis followed by determination of dysregulated proteins through Coomassie blue staining-based densities (SameSpots software) and subsequent protein identification using MALDI-based mass spectrometry. Expression differences in dysregulated proteins were found to be statistically significant with p-value <0.05. A systems biology-based approach was utilized to determine critical biochemical pathways involved in the rats' brain response. Our results suggest that rats' brains have a particulate matter size dependent-response, being the mitochondrial activity and the astrocyte function severely affected. Our proteomic study confirms the dysregulation of different biochemical pathways involving energy metabolism, mitochondrial activity, and oxidative pathways as some of the main effects of PM exposure on the rat brain. Significance: Rat brains exposed to particulate matter with origin in car engines are affected in two main areas: mitochondrial activity, by the dysregulation of many pathways linked to the respiratory chain, and neuronal and astrocytic function, which stimulates brain changes triggering tumorigenesis and neurodegeneration. (C) 2019 Published by Elsevier B.V.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available