Article
Engineering, Environmental
Yue Wu, Baocai Zhang, Yuxuan Wan, Yongheng Jiang, Nan Li, Xinlei Jiang, Chengmei Liao, Xin Wang
Summary: This study demonstrates the feasibility of using iron to reduce nitrate to ammonium and proves the effectiveness of nitrate as an electron acceptor for enriching Geobacter. This finding provides a new route for autotrophic nitrate removal and recycling, with broader implications on nitrogen and iron cycling in biogeochemical processes.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Abid Hussain, Jangho Lee, Hao Ren, Hyung-Sool Lee
Summary: This study evaluated the spatial distribution of biofilm conductivity (K-bio) on an anodic biofilm at different growth conditions. The increase in K-bio was associated with an increase in current density. Geobacter was the dominant microbial community on all electrode pairs. Acetate concentration significantly influenced the spatial distribution of K-bio.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Environmental Sciences
Yifei Wang, Yaohuan Gao, Abid Hussain, Hyung-Sool Lee
Summary: This study aimed to develop a standardized and consistent method for measuring biofilm conductance to enhance the understanding of extracellular electron transfer. The results showed no significant difference in biofilm conductance with and without a fixed anode potential. The conductance exhibited a sigmoidal relationship with anode potential and deviated from Ohm's law at applied voltages larger than 100 mV. Increasing measurement time led to a decrease in biofilm conductance and further deviation from Ohm's law. By optimizing operation settings, such as voltage range, step, measurement time, and anode control mode, more accurate and consistent biofilm conductance measurement was achieved in the 2-Au MEC system. This study provided a comprehensive evaluation of environmental and instrumental parameters for biofilm conductance measurement.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Zheng Zhuang, Guiqin Yang, Li Zhuang
Summary: The study investigated the role of exopolysaccharides in electroactive biofilms by constructing an exopolysaccharides-deficient Geobacter sulfurreducens-based BES. The findings demonstrated the essential role of exopolysaccharides in extracellular electron transfer (EET) and early biofilm formation, with pili playing a compensatory role in biofilm formation. The mutant biofilm had lower cell viability, thinner biofilm, and decreased electrochemical activity compared to the wild-type biofilm.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Nanoscience & Nanotechnology
Yunfei Li, Jia Liu, Xuepeng Chen, Jingxuan Wu, Nan Li, Weihua He, Yujie Feng
Summary: In this study, an efficient N- and Fe-abundant carbon cloth (CC) electrode was synthesized and developed as an anode (named FePor-PQ) in microbial fuel cells (MFCs). The FePor-PQ anode achieved a maximum power density of 2165.7 mW m(-2), surpassing other types of electrodes and significantly enriching exoelectrogens. This work demonstrates the synergistic effect of heteroatom coating and surface properties tailoring to enhance both EET efficiency and exoelectrogen enrichment in MFCs, providing valuable insights for designing electrodes in other bio-electrochemical systems.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Environmental
Wei Guo, Xianbin Ying, Nannan Zhao, Shengsong Yu, Xu Zhang, Huajun Feng, Yifeng Zhang, Hanqing Yu
Summary: Groundwater nitrate pollution is a severe environmental problem worldwide. Autotrophic denitrification on polarized electrodes, using biofilms consisting of symbiotic growth of Geobacter and denitrifying bacteria, has shown a significantly higher nitrogen removal rate compared to traditional methods. The excellent performance of the biofilms is attributed to the electron transfer ability between Geobacter and denitrifying bacteria, which was verified by suppressing current generation and denitrification through inhibitors. This study reveals a new extracellular electron transfer mechanism and offers insights into the development of efficient biocathodes for treating nitrate pollution in groundwater.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Environmental
Xing Liu, Ji Zhan, Lu Liu, Feiting Gan, Jie Ye, Kenneth H. Nealson, Christopher Rensing, Shungui Zhou
Summary: This study introduces an in situ anaerobic spectroelectrochemical coculture cell (ASCC) to induce the formation of direct interspecies electron transfer (DIET) and characterize it. Through observing two typical Geobacter DIET cocultures, it was found that cytochromes are abundant in DIET coculture, indicating a cytochrome-mediated electron transfer mechanism.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Toshiyuki Ueki
Summary: Geobacter species are important in environmental biogeochemical cycles and biotechnology applications due to their capability of direct EET with solid electron donors and acceptors. They have a large number of cytochromes encoded in their genomes, localized in various subcellular fractions and utilized differentially for EET with various electron donors and acceptors. Geobacter coordinates the cytochromes with other cellular components in an elaborate EET system to thrive in the environment.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marta A. A. Silva, Ana P. P. Fernandes, David L. L. Turner, Carlos A. A. Salgueiro
Summary: Periplasmic nanowires and electric conductive filaments made of c-type cytochromes from Geobacter sulfurreducens bacterium are crucial for electron storage and/or extracellular electron transfer. The assignment of the heme proton NMR signals for domains C and D in the nanowire cytochrome GSU1996 was obtained and used to guide the assignment of the corresponding signals in the hexaheme bi-domain CD. This new strategy provides a way to functionally characterize large multiheme cytochromes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Chao Li, Yujie Feng, Dandan Liang, Lijuan Zhang, Yan Tian, Ravi Shankar Yadav, Weihua He
Summary: This study successfully developed a spatial electroactive biofilm that greatly improved the performance of the bioanode in microbial fuel cells. Compared with traditional electrodes, this electrode had higher coulombic recovery and lower barriers to electron and mass transfer. Furthermore, the electrode preparation technology used in this study significantly reduced the cost, making it more feasible for the construction of pilot-scale MFC equipment.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Microbiology
Jinjie Zhou, Jessica A. Smith, Meng Li, Dawn E. Holmes
Summary: Methanothrix plays a major role in global methane emissions and can form methane from acetate dismutation and direct interspecies electron transfer (DIET). This study used transcriptomics to identify potential routes of electron transfer during DIET between Geobacter metallireducens and Methanothrix thermoacetophila. The results provide important insights into the physiology of Methanothrix in anoxic environments and potential electron transfer mechanisms during DIET.
Article
Biology
Fengbin Wang, Chi Ho Chan, Victor Suciu, Khawla Mustafa, Madeline Ammend, Dong Si, Allon Hochbaum, Edward H. Egelman, Daniel R. Bond
Summary: Early genetic and structural observations suggested that metal-reducing organisms like Geobacter had extracellular conductive filaments composed of type IV pili. However, recent research has shown that bacterial c-type cytochromes can also form extracellular filaments for long-range electron transport. Cryo-electron microscopy was used to determine the structure of a third extracellular filament, formed from Geobacter sulfurreducens octaheme cytochrome, OmcZ. The structure of OmcZ differs from that of OmcS and OmcE, indicating that conductive cytochrome polymers likely arose independently from different ancestral multiheme proteins.
Article
Engineering, Environmental
Xing Liu, Yin Ye, Zhishuai Zhang, Christopher Rensing, Shungui Zhou, Kenneth H. Nealson
Summary: This study reveals that lysogenic phages can cause decay of electroactive biofilms (EAB) in Geobacter sulfurreducens fuel cells, leading to a decline in current generation. The presence of prophages on the G. sulfurreducens genome was confirmed, and the transition of those prophages from lysogenic to lytic was observed, resulting in the progressive decay of the EAB. The addition of phages from decayed EAB accelerated the decay process, while deleting prophage-related genes rescued the decay process.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Microbiology
Yin Ye, Xing Liu, Kenneth H. Nealson, Christopher Rensing, Shuping Qin, Shungui Zhou
Summary: Conductive nanowires, such as pili, OmcS, and OmcZ, play important roles in long-range electron transfer in Geobacter sulfurreducens anode biofilms. Pili contribute to biofilm formation, OmcS facilitates electron transfer, and OmcZ contributes to both biofilm formation and current generation. Understanding the mechanism of electron transfer can help increase the power density of microbial fuel cells.
Article
Engineering, Environmental
Yue Lu, Qingqing Xie, Lin Tang, Jiangfang Yu, Jingjing Wang, Zhaohui Yang, Changzheng Fan, Shoujuan Zhang
Summary: In this study, the addition of Fe-bearing sludge-derived biochar combined with Geobacter sulfurreducens accelerated the reduction of NB, forming biochar with redox-active centers. Through the microbial dissimilatory iron reduction mechanism, the biotic and abiotic reduction rates of NB were effectively improved.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biophysics
Gretchen Tibbits, Abdelrhman Mohamed, Douglas R. Call, Haluk Beyenal
Summary: This study proposes a rapid and direct method for classifying antibiotic susceptibility using mediated extracellular electron transfer, effectively categorizing bacteria as either resistant or susceptible.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Engineering, Environmental
Young Eun Song, Abdelrhman Mohamed, Changman Kim, Minsoo Kim, Shuwei Li, Eric Sundstrom, Haluk Beyenal, Jung Rae Kim
Summary: Microbial electrosynthesis (MES) is a promising method to convert inorganic gaseous carbon into valuable chemicals through electrode-based electron transfer. The addition of artificial mediators improves acetate synthesis, and regular media replacement helps adapt mediator-utilizing species. Biofilm composition and interaction with suspended cells play a crucial role in MES performance.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Iris Pinheiro, Aurora Barbera, Imma Raurell, Federico Estrella, Marcel de Leeuw, Selin Bolca, Davide Gottardi, Nigel Horscroft, Sam Possemiers, Maria Teresa Salcedo, Joan Genesca, Maria Martell, Salvador Augustin
Summary: The present study demonstrated that oral administration of a specific bacterial consortium improved disease outcomes in two rodent models of non-alcoholic fatty liver disease. The consortium was found to effectively replace and enrich the gut microbiome, leading to improvements in endothelial function, fibrosis, and disease progression.
Article
Biology
Stacy Simai Reginald, Hyeryeong Lee, Nabilah Fazil, Basit Sharif, Mungyu Lee, Min Ji Kim, Haluk Beyenal, In Seop Chang
Summary: This study demonstrates the control of carbon monoxide dehydrogenase orientation on electrode surfaces and suggests its application for promoting direct electron transfer in bioelectrocatalytic systems.
COMMUNICATIONS BIOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Abdelrhman Mohamed, Yash S. Raval, Suzanne Gelston, Gretchen Tibbits, Suat U. Ay, Laure Flurin, Kerryl E. Greenwood-Quaintance, Robin Patel, Haluk Beyenal
Summary: Chronic wound biofilm infections are a major challenge in clinical practice. Topical antibiotic treatment is often ineffective due to resistance and tolerance of microorganisms within biofilms. The use of biocides such as hypochlorous acid (HOCl) has gained attention as a potential solution. A wearable electrochemical bandage (e-bandage) that generates HOCl is developed and shows promising results against biofilms of Acinetobacter baumannii and Staphylococcus aureus.
ADVANCED ENGINEERING MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Gretchen Tibbits, Abdelrhman Mohamed, Suzanne Gelston, Laure Flurin, Yash S. Raval, Kerryl E. Greenwood-Quaintance, Robin Patel, Haluk Beyenal
Summary: The activity of an electrochemical bandage producing hypochlorous acid (e-bandage) in preventing and removing biofilms formed by methicillin-resistant Staphylococcus aureus (MRSA) was assessed using a porcine explant biofilm model. The results showed that the e-bandage was effective in inhibiting MRSA infection and reducing the viable cell counts of MRSA biofilms.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Gretchen Tibbits, Abdelrhman Mohamed, Suzanne Gelston, Laure Flurin, Yash S. Raval, Kerryl Greenwood-Quaintance, Robin Patel, Haluk Beyenal
Summary: This study evaluated the effects of H2O2 producing electrochemical-bandages on MRSA colonization and biofilm removal. The results showed that the electrochemical-bandages effectively reduced S. aureus colonization and young biofilms, with limited effects on mature biofilms.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Juan M. Pericas, Frank Tacke, Quentin M. Anstee, Nicholas A. Di Prospero, Mette Skalshoj Kjaer, Peter Mesenbrink, Franz Koenig, Joan Genesca, Vlad Ratziu, EU-PEARL NASH Invest
Summary: Non-alcoholic fatty liver disease affects 25% of the population and non-alcoholic steatohepatitis (NASH) is a progressive form of the disease with severe complications. The traditional clinical trials in NASH pose challenges for drug development, and platform trials, utilizing a master protocol and adaptive randomization, offer an alternative approach to evaluate multiple investigational medicinal products concurrently or sequentially.
JOURNAL OF HEPATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Md Monzurul Islam Anoy, Suzanne Gelston, Abdelrhman Mohamed, Laure Flurin, Yash S. Raval, Kerryl Greenwood-Quaintance, Robin Patel, Zbigniew Lewandowski, Haluk Beyenal
Summary: This study characterizes the reactions occurring on the counter electrode of an electrochemical bandage and finds that the production of hypochlorous acid (HOCl) on the counter electrode enhances its antibiofilm activity.
BIOELECTROCHEMISTRY
(2022)
Article
Gastroenterology & Hepatology
Ian Rowe, Candid E. Villanueva, Jessica Shearer, Ferran Torres, Agustin Albillos, Joan C. Genesca, Joan Garcia-Pagan, Dhiraj C. Tripathi, Peter Hayes, Jaume G. Bosch, Juan Abraldes
Summary: This study conducted a Bayesian reanalysis of PREDESCI and found that beta-blocker treatment is highly likely to prevent decompensation and provides a substantial gain in decompensation-free life years at the population level.
Article
Electrochemistry
Daniel E. Molina, Nathalie A. Wall, Haluk Beyenal, Cornelius F. Ivory
Summary: We used an improved version of our hyphenated analysis technique, flow injection electrochemical quartz crystal microbalance with inductively coupled optical emission spectroscopy (FI-EQCM-ICP-OES), to investigate the electroprecipitation of lanthanum and neodymium from flowing solutions. The improved technique has two independent flow injection circuits, allowing different conditions for electrochemical precipitation and quick stripping. Lanthanum or neodymium ions were injected into a carrier stream and deposited onto an electrochemical quartz crystal microbalance (EQCM), and the deposits were subsequently stripped and analyzed using an ICP-OES. We found that using acidic solutions without supporting electrolyte leads to an increase in the amount of lanthanum detected.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Article
Electrochemistry
Jeffrey Eakin, Daniel Molina, Xiaofeng Guo, Haluk Beyenal, Cornelius Ivory
Summary: Electrochemical recovery of dilute concentrations of actinides from spent nuclear fuel is achieved using electrochemical deposition with redox replacement (EDRR) in a molten salt medium. The process selectively recovers uranium in the presence of excess concentrations of lanthanum. The recovery involves replacing the deposited lanthanum with uranium after each EDRR cycle.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Article
Pharmacology & Pharmacy
Yash S. Raval, Derek Fleming, Abdelrhman Mohamed, Melissa J. Karau, Jayawant N. Mandrekar, Audrey N. Schuetz, Kerryl E. Greenwood-Quaintance, Haluk Beyenal, Robin Patel
Summary: A novel electrochemical bandage composed of carbon fabric and controlled by a wearable potentiostat is evaluated for its effectiveness against antibiotic-resistant bacterial biofilms in wound infections. The bandage, which continuously delivers low amounts of hydrogen peroxide, shows significant reductions in both monospecies and mixed-species wound infections without causing adverse reactions.
ADVANCED THERAPEUTICS
(2023)
Letter
Gastroenterology & Hepatology
Jesus Rivera-Esteban, Aurora Barbera, Maria Teresa Salcedo, Maria Martell, Joan Genesca, Juan M. Pericas
Article
Gastroenterology & Hepatology
Anahita Rabiee, Yanhong Deng, Maria Ciarleglio, Jean L. Chan, Monica Pons, Joan Genesca, Guadalupe Garcia-Tsao
Summary: Clinically significant portal hypertension (CSPH) is important in identifying the risk of decompensation in patients with compensated cirrhosis. The ANTICIPATE models and a new laboratory-based model have shown promise in predicting CSPH in NASH cirrhosis.
HEPATOLOGY COMMUNICATIONS
(2022)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
