Journal
BIOSENSORS & BIOELECTRONICS
Volume 86, Issue -, Pages 536-541Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.07.025
Keywords
Electrochemical aptasensor; Nanoprobe; Terminal deoxynucleotidyl transferase (TdT); Aptamer-initiated on-particle template-independent enzymatic polymerization (aptamer-OTEP)
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Funding
- National Natural Science Foundation of China [61371039, 91123037, 21305151, 31470960]
- National Basic Research Program (973 Program) [2012CB932600]
- Fundamental Research Funds for the Central Universities [30915118835, 30916011201]
- Distinguished Scientific Fellowship Program at King Saud University
- Chinese Academy of Sciences
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Herein, an aptamer-initiated on-particle template-independent enzymatic polymerization. (aptamer-OTEP) strategy for electrochemical aptasensor (E-aptasensor) is developed for analysis of cancer biomarker carcino-embryonic antigen (CEA). A pair of DNA aptamers is employed which can be specifically bond with CEA simultaneously. One of the aptamer is thiolated at 3'-terminal and immobilized onto the gold electrode as a capture probe, while the other one has a thiol group at its 5'-terminal and is modified onto the gold nanoparticles surface to form a nanoprobe. In the present of target, the two aptamers can sandwich the target, thus the nanoprobe is attached to the electrode. Then terminal deoxynucleotidyl transferase (TdT) is employed to catalyze the incorporation of biotin labeled dNTPs into the 3'-OH terminals of the DNA aptamer on the nanoprobe. The as-generated long DNA oligo tentacles allow specific binding of numerous avidin modified horseradish peroxidase (Av-HRP), resulting in tens of thousands of HRP catalyzed reduction of hydrogen peroxide and sharply increasing electrochemical signals. Taking advantage of the enzyme based nucleic acid amplification and nanoprobe, this strategy is demonstrated to possess the outstanding amplification efficiency. (C) 2016 Elsevier B.V. All rights reserved.
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