Article
Chemistry, Analytical
Jung Ho Kim, Seokjoon Kim, Sung Hyun Hwang, Tae Hwi Yoon, Jung Soo Park, Eun Sung Lee, Jisu Woo, Ki Soo Park
Summary: Water and food contaminated by pathogens are major causes of diseases and deaths globally. A system is needed to control pathogen contamination and quickly detect and monitor target pathogens. The three-way junction-induced transcription amplification can detect target nucleic acids effectively.
Article
Chemistry, Multidisciplinary
Guobin Han, Dandan Li, Qiuyuan Lin, Jia Yi, Qian Lyu, Qingwei Ma, Liang Qiao
Summary: In this work, a method combining EXPAR and MALDI-TOF MS is developed for label-free and simultaneous detection of multiple low-abundance miRNAs. The method can be performed in a single reaction tube under isothermal conditions and completed in less than 30 minutes. It demonstrates good quantification ability, attomolar-level sensitivity, and high specificity for miRNAs detection, including single-nucleotide resolution. Therefore, this label-free mass-spectrometric platform has the potential to be a valuable tool for miRNA analysis in complex biological samples and clinical diagnosis.
CHINESE CHEMICAL LETTERS
(2023)
Article
Biophysics
Hyo Yong Kim, Jayeon Song, Hyun Gyu Park
Summary: An ultrasensitive isothermal method for detecting microRNA using target-induced chain amplification reaction (CAR) is described. The strategy involves a hairpin probe (HP) that opens upon binding to target miRNA, leading to exponential amplification reactions and the production of double-stranded DNA products that can be monitored through fluorescent signals.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Chenqi Niu, Juewen Liu, Xinhui Xing, Chong Zhang
Summary: In this study, a novel strategy was proposed to eliminate the non-specific signal from non-specific amplification by integrating the CRISPR-Cas12a system into two-templates exponential amplification reactions (EXPAR). The developed EXPAR-Cas12a strategy named EXPCas acted as a filter to filter out non-specific amplificons and improved the signal-to-background ratio significantly. With miRNA-21 as the target, the detection could be accomplished in 40 min with a LOD of 103 fM and no non-specific amplification was observed.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Hao Jiang, Yuan Li, Xuefei Lv, Yulin Deng, Xiaoqiong Li
Summary: Nucleic acid amplification techniques, such as polymerase chain reaction (PCR) and isothermal amplification, are important for nucleic acid detection. However, PCR is limited by temperature control and isothermal amplification has its own limitations. Efforts to integrate enzymes and amplification techniques have the potential to overcome these limitations. This review provides a comprehensive summary of cascade amplification, including design fundamentals, signal generation, evolution, applications, challenges, and trends.
Article
Chemistry, Analytical
Mengxu Sun, Yanxin Zhang, Juan Xie, Ya Zhang, Ting Huang, Minmin Li, Jin-Xiang Chen, Zong Dai, Jun Chen
Summary: This study developed a highly efficient and sensitive miRNA detection method by combining APE1-mediated isothermal target cycling amplification system with magnetic separation. The label-free and rapid detection of miRNA was achieved through the recognition and cleavage of AP probes by APE1, and the fluorescence measurement of G4/ThT. The method showed high specificity and sensitivity, and demonstrated its application in detecting miR-1246 in serum samples from breast cancer patients and normal people.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Biochemical Research Methods
Zhigang Luo, Shuang Zhang, Qing Feng, Ya Zhou, Lian Jin, Jinqiu Sun, Yunfeng Chen, Kun Jia, Lei Chu
Summary: Sensitive and reliable quantitative determination of microRNA (miRNA) is crucial for disease diagnosis and cancer treatment. A novel miRNA detection approach based on target recognition initiated self-dissociation of DNA nanomachine is proposed. The method exhibits high sensitivity with a low detection limit of 12 fM and high selectivity that can distinguish one mismatched base pair. This approach is believed to be a robust tool for early cancer diagnosis and anticancer drug development.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Biochemical Research Methods
Chunxu Yu, Siyan Zhou, Xin Zhao, Yidan Tang, Lina Wang, Baiyang Lu, Fanzheng Meng, Bingling Li
Summary: The nucleic acid testing technology has advanced in recent years, but there are still challenges in the clinical application of multiple nucleic acid assays, such as accuracy, speed, and cost. This paper introduces a three-way junction structure that can translate analytes of loop-mediated isothermal amplification to a catalytic hairpin assembly. With a well-optimized nucleic acid circuit, multiple and logical analysis can be easily achieved in a single reaction with high sensitivity and selectivity. The experimental results of clinical samples support the potential clinical application of this methodology.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yi He, Zhiyou Zeng, Yongguo Cao, Xiao Zhang, Caijun Wu, Xiaojun Luo
Summary: In this study, a simple and sensitive SERS biosensor was proposed for ultrasensitive detecting miRNA 122 based on ZnO nanoparticle amplification strategy and the full utilization of DNA chain. The biosensor showed a low detection limit and a wide linear range, indicating great potential in clinical application and medical diagnosis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Yihan Wang, Huan Feng, Jinfeng Quan, Ke Huang, Jinpeng Wang, Xiaohui Liu, Hui Jiang, Xuemei Wang
Summary: In this study, an enzyme-assisted cyclic amplification strategy was developed for the electrochemical detection of microRNAs (miRNAs) in serum. The strategy achieved highly sensitive detection of miRNAs with a low limit of detection through a catalytic hairpin assembly (CHA) reaction. This method has the potential for clinical miRNA analysis.
ANALYTICA CHIMICA ACTA
(2022)
Article
Multidisciplinary Sciences
Jake G. Carter, Lorea Orueta Iturbe, Jean-Louis H. A. Duprey, Ian R. Carter, Craig D. Southern, Marium Rana, Celina M. Whalley, Andrew Bosworth, Andrew D. Beggs, Matthew R. Hicks, James H. R. Tucker, Timothy R. Dafforn
Summary: A rapid isothermal method for detecting SARS-CoV-2 has been developed, using a reverse transcription-free approach to convert RNA into DNA and rapidly amplifying it. The assay is faster than traditional methods like RT-qPCR and RT-LAMP, while maintaining sensitivity, making it a promising tool for detecting RNA-based pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Analytical
Yi Sun, Sicai Zhang, Lijuan Qi, Xiaojun Zhang, Meiting Yang, Zhijun Guo, Zhenxin Wang, Yan Du
Summary: This study developed a novel multivariate real-time fluorescent probe called MRTFP, which allows for efficient and accurate disease detection and pathogen identification. By incorporating strand exchange three-way-junction structures into four-angle and hexagonal probes, single-step and multiplexed detection with exceptional sensitivity and specificity were achieved. The introduction of MRTFP simplifies probe design and improves detection efficiency.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Qiaomin Wu, Xinni Xiang, Yixiao Yuan, Yang Yu, Mengqi Chen, Jinyan Long, Tingxiu Xiang, Xiaolan Yang
Summary: DNA methylation is important for cancer detection, and this study identified CLDN11 methylation as a valuable marker for lung adenocarcinoma diagnosis and prognosis. A flexible platform was proposed for ultrasensitive detection of CLDN11 methylation by integrating GlaI cleavage, EXPAR, and CRISPR/Cas12a. The platform achieved excellent performance and can be used for the quantification of human serum and genomic DNA, as well as distinguishing normal cells from cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Biochemistry & Molecular Biology
Siyu Cao, Xiaochen Tang, Tianshu Chen, Guifang Chen
Summary: This article reviews the principles, advantages and disadvantages, and applications of several nicking enzyme-combined isothermal amplification techniques, as well as the prospects for their development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
He Yan, Yunjie Wen, Zimu Tian, Nathan Hart, Song Han, Steven J. Hughes, Yong Zeng
Summary: A one-pot isothermal assay utilizing rolling-circle amplification and the endonuclease Cas12a can accurately detect specific miRNAs in extracellular vesicles in patient plasma. The assay is rapid, does not require specialized instrumentation, and is compatible with smartphone-based fluorescence detection and lateral-flow format for visual readouts. These simple assays may aid in the development of miRNAs as biomarkers for cancer diagnosis and prognosis.
NATURE BIOMEDICAL ENGINEERING
(2023)
Article
Chemistry, Analytical
Chuan Huang, Xuefeng Wang, Zuhao Zhang, Lu Zhang, Dejin Zang, Shenguang Ge, Jinghua Yu
Summary: In this study, a novel anode-cathode cooperative paper photoelectrochemical platform was fabricated for ultrasensitive monitoring of miRNA155 using different conductive semiconductors. The platform exhibited excellent sensitivity and selectivity based on the multifold amplification mechanisms and cathode detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Lin Li, Hongmei Yang, Li Li, Xiaoran Tan, Shenguang Ge, Lina Zhang, Jinghua Yu, Yan Zhang
Summary: This study develops a versatile collapsible lab-on-paper biosensor with photothermal effects for early-stage diagnosis and real-time surveillance of epidemic diseases. By implanting a zone with photothermal effects in the miniature space, it enables the on-site detection of microRNA-141 in body fluids and interconnects the traditional photocurrent signal with functional temperature response. The use of Fe3O4 nanoparticles and Prussian blue nanoparticles enhances the visualized thermoresponsive results. Control release of Fe3O4 nanoparticles and modulation of photoelectric signal attenuation improve the accuracy of target temperature signal prediction and enable ultrasensitive detection results.
Article
Chemistry, Analytical
Fengyi Wang, Yaqi Liu, Lu Zhang, Zuhao Zhang, Chuan Huang, Dejin Zang, Haiqing Wang, Shenguang Ge, Jinghua Yu
Summary: A high-sensitivity photoelectrochemical biosensor based on CdS quantum dots sensitized porous hexagonal boron nitride nanosheets and a multiple sites tripodal DNA walker was developed. The biosensor showed excellent linear relationship and detection limit, providing a powerful strategy for early clinical diagnosis and biomedical research.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Yanhu Wang, Mengchun Yang, Shenguang Ge, Xiao Wang, Jinghua Yu
Summary: This study developed a highly sensitive photoelectrochemical (PEC) strategy for monitoring exosomal miRNAs by utilizing the piezoelectric effect and a metal-organic framework-capped CaO2. The strategy showed promising potential for real-time analysis of exosomal miRNAs.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Yaqi Liu, Fengyi Wang, Shuo Ge, Lu Zhang, Zuhao Zhang, Yunqing Liu, Yan Zhang, Shenguang Ge, Jinghua Yu
Summary: A strand displacement amplification (SDA)-assisted CRISPR/Cas12a electrochemiluminescence (ECL) biosensor was developed for ultrasensitive detection of Staphylococcus aureus (Sa)-16S rDNA. The biosensor utilized a porphyrinic Zr metal-organic framework (MOF) as a coreactant accelerator to enhance the ECL emission signal. The presence of Sa-16S rDNA triggered the SDA reaction, generating single-stranded DNA products (SP) that activated the CRISPR/Cas12a trans-cleavage and effectively extinguished the ECL signals. The ECL platform exhibited high sensitivity and specificity, making it suitable for clinical diagnosis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Qingxin Zhang, Manru Zhang, Zhiqiang Guo, Jingjing Li, Zhixue Zhu, Yu Wang, Su Liu, Jiadong Huang, Jinghua Yu
Summary: In this study, a low-background electrochemical biosensor based on a DNA tetrahedron-besieged primer and a DNAzyme-activated programmatic rolling circle amplification (RCA) was proposed for OTA detection in wine samples. The low-background detection was achieved by using the besieged primer and the target OTA-mediated DNAzyme activation initiated the programmatic RCA. The integration of the catalytic property of silver nanoclusters (AgNCs) with the electrochemical assay provided high sensitivity for OTA detection. This novel design showed great potential in food safety and environmental detection.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Xu Li, Jiali Huang, Jiayu Ding, Mingzhen Xiu, Kang Huang, Kang Cui, Jing Zhang, Shiji Hao, Yan Zhang, Jinghua Yu, Yizhong Huang
Summary: In this study, a dual-mode lab-on-paper device was constructed using BiVO4/FeOOH nanocomposites as an efficient photoelectrochemical (PEC)/colorimetric signal reporter for sensitive detection of carcinoembryonic antigen (CEA). The nanocomposites with an FeOOH needle-like structure displayed enhanced PEC response activity, enabling quantitative detection of CEA with the PEC signal readout mode. Additionally, a colorimetric signal readout system was achieved through a smart folding technique, providing a dual-mode signal readout for the lab-on-paper device.
Article
Chemistry, Analytical
Qian Wang, Liang Lv, Wenhao Chi, Yujiao Bai, Wenqing Gao, Peihua Zhu, Jinghua Yu
Summary: A new porphyrin-based porous covalent organic framework (TAP-COF) with high surface areas and efficient electron transport was synthesized. The TAP-COF exhibited excellent peroxidase-like catalytic activity and was able to catalyze the oxidation of a substrate by hydrogen peroxide. Simple and rapid colorimetric methods for in situ hydrogen peroxide detection were developed using this TAP-COF nanozyme, which has potential applications in point-of-care testing, cell biology, and clinical diagnosis.
Article
Chemistry, Analytical
Huihui Shi, Yanfei Che, Yumeng Rong, Jiajun Wang, Yanhu Wang, Jinghua Yu, Yan Zhang
Summary: An integrated dual-signal bioassay was developed for ultrasensitive detection of thrombin. This method combined visualization with photoelectrochemical technique to achieve the detection. The bioassay demonstrated wide linear ranges, high selectivity, stability, and reproducibility under optimized conditions. Additionally, the proposed visual/photoelectrochemical transduction mechanism platform offered a lower background signal and more reliable detection results, providing an effective way for detecting other proteins and nucleic acids.
Article
Chemistry, Analytical
Wenli Xiu, Peini Zhao, Yujie Pan, Xiaoru Wang, Lina Zhang, Shenguang Ge, Jinghua Yu
Summary: A surface-enhanced Raman scattering (SERS) sensor was developed for rapid on-site determination of ethephon in food, based on the folding and assembly characteristics of paper fibers, the skeleton controllability of metal-organic framework materials (MOFs), and the morphology designability of plasmonic noble metal materials. The sensor used HKUST-1(Cu) assembled onto a carbon-treated chromatographic paper matrix to overcome poor affinity of SERS substrate for target molecules. With the coupling of biomimetic antibodies and a multi-stage progressive enhancement structure, the sensor achieved high specificity and sensitivity for ethephon detection with a linear range of 10-3 to 10 mg kg-1 and a low detection limit of around 1.39 x 10-4 mg kg-1.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Li Shan, Yuanyuan Chen, Xiaoran Tan, Shenguang Ge, Lina Zhang, Lin Li, Jinghua Yu, Li Li
Summary: A newly designed two-in-one tetrahedral DNA (TDN) nanostructure with an antifouling surface and backbone-rigidified interfacial tracks was developed for highly sensitive and selective detection of miRNA-182-5p. The TDN-modified sensing interfaces demonstrated incomparable antifouling ability against protein samples and interfering miRNAs due to the strong hydrophilic capacity and special molecular conformations. The designed TDN-modified paper-based photoelectro-chemical sensor successfully quantified miRNA-182-5p with a low detection limit of 0.09 fM and high specificity in real samples, showing great promise for bioanalysis and clinical diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Shanshan Li, Haihan Yu, Yuwen Wang, Shuai Wang, Lina Zhang, Peihua Zhu, Chaomin Gao, Jinghua Yu
Summary: A stable organic-inorganic Z-scheme heterostructure was constructed by covalently connecting COF-366-Fe and CeO2 through Schiff base reaction, which effectively integrated their respective properties and improved the separation and transfer of photoexcited carriers. The COF-366-Fe@CeO2 exhibited superior photocatalytic CO yield compared to COF-366-Fe and CeO2, indicating its potential as a highly-effective COF-based photocatalyst for artificial photosynthesis.
APPLIED SURFACE SCIENCE
(2023)
Article
Biophysics
Lin Li, Hui Tian, Guofu Wang, Suyue Ren, Tinglei Ma, Yixiang Wang, Shenguang Ge, Yan Zhang, Jinghua Yu
Summary: Ready-to-use in vitro diagnosis is crucial for cervical cancer prevention and treatment. A paper-film-based differential lateral flow biosensor is proposed, allowing simultaneous detection of two high-risk HPV types. The biosensor utilizes a CuCo2S4/ZnIn2S4 heterostructure on a paper-film chip and introduces surface sulfur vacancy for enhanced photoexcitation. Through sequential solution transfer, the biosensor activates the self-assembly of DNA and the cleavage activity of CRISPR-Cas12a, resulting in the release of polydopamine-coated gold nanoparticles. This intelligent biosensor achieves low limit-of-detection for HPV-18 and HPV-16 and provides a promising approach for home medical intelligent diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Physical
Yunqing Liu, Zuhao Zhang, Lu Zhang, Yuguo Xia, Haiqing Wang, Hong Liu, Shenguang Ge, Jinghua Yu
Summary: Integrating bifunctional electrocatalysts into one electrolytic cell for water electrolysis has demonstrated the convenience and efficiency in green hydrogen production. This study developed a dual metal doping strategy and successfully designed a high-performance bifunctional electrocatalyst for robust water splitting, achieving both oxygen evolution reaction and hydrogen evolution reaction at low overpotentials in alkaline media. The collaboration approach of codoped low-valence and high-valence metals shows great potential in the development of high-performance and versatile catalysts.
JOURNAL OF MATERIALS CHEMISTRY A
(2022)
Article
Chemistry, Analytical
Chuan Huang, Lu Zhang, Yuanna Zhu, Zuhao Zhang, Yunqing Liu, Chao Liu, Shenguang Ge, Jinghua Yu
Summary: This study presents a novel double-engine powered paper photoelectrochemical biosensor for the monitoring of multiple miRNAs. The biosensor utilizes various signal enhancement mechanisms and a flexible paper chip design to amplify signals and enable multitarget detection. The use of a DNA nanomachine and CRISPR/Cas12a shearing tool allows for signal quenching and simplified operation. The biosensor demonstrates excellent selectivity and sensitivity, making it a valuable tool for trace multitarget monitoring in clinical applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)