Article
Engineering, Biomedical
Chang Liu, Hui Yang, Nai-An Shen, Juehong Li, Yunsu Chen, Jin-Ye Wang
Summary: By adding electrospun zein fibers and quenching treatment, the mechanical properties of zein-based scaffolds were significantly improved, including compressive, tensile, and bending strengths. Both modification methods had a significant impact on the properties of the material, indicating potential applications in bone repair.
BIOMEDICAL MATERIALS
(2021)
Review
Engineering, Biomedical
Kenichi Arai, Takahiro Kitsuka, Koichi Nakayama
Summary: The safety and efficacy of new drugs are typically tested in animals, but differences in drug responses and potential cardiac side effects have led to many drug development projects being discontinued. Alternative methods to animal testing are needed. Tissue engineering techniques can create three-dimensional cardiac constructs from hiPSC-CMs, providing a new approach for drug screening.
Article
Pharmacology & Pharmacy
Mohammad Foad Abazari, Sepehr Torabinejad, Shohreh Zare Karizi, Hadi Samadian, Sahar Jalili-ghelichi, Mehdi Norouzi, Hassan Askari, Vahdat Poortahmasebi, Seyedeh Elnaz Enderami, Fatemeh Soleimanifar
Summary: This study demonstrates the high osteo-supportive capacity of a modified nanofibrous scaffold using human placental extract. It provides a potential candidate for bone tissue engineering.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shanzhu Guo, Yue Jiang, Jingjing Jiao, Yan Shi, Tao Zhu, Long Li
Summary: In this study, a gelatin-based biomimetic three-layer vascular scaffold with spatial alignment features mimicking the natural structure of blood vessels was successfully constructed using a sequential electrospinning strategy combined with folding and rolling manipulation. The scaffold could fully mimic the natural multi-layer structures of blood vessels and showed great potential for guiding the spatial arrangement of cells in blood vessels.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Materials Science, Biomaterials
Kanchan Maji, Krishna Pramanik
Summary: The growing trend of bone disorders caused by accidental injury and the increasing aging population demand more effective therapeutic intervention. Bone tissue engineering offers functional bone grafts, and electrospinning technology provides a potential solution to the limitations of traditional bone grafts, but scaffold matrix fabrication remains a significant challenge.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2022)
Article
Cell & Tissue Engineering
Angela Semitela, Goncalo Ramalho, Ana Capitao, Catia Sousa, Alexandrina F. Mendes, Paula A. A. P. Marques, Antonio Completo
Summary: Electrospinning is widely used in fabricating fibrous scaffolds for cartilage tissue engineering. However, small pores in the scaffold restrict cell infiltration. Bio-electrospraying provides a solution by incorporating chondrocytes into the fibers during electrospinning. Through experiments, it was found that chondrocytes survived and functioned well under certain electrospraying conditions. The combination of electrospraying and polymer electrospinning shows promising results for the delivery and development of cellularized cartilage tissue constructs.
JOURNAL OF TISSUE ENGINEERING
(2022)
Article
Engineering, Biomedical
Wei Chen, Yongsheng Li, Yuting Huang, Yao Dai, Tingfei Xi, Zheng Zhou, Hairong Liu
Summary: The study indicates that quercetin-modified PHBV fibrous scaffold has the potential to promote cartilage regeneration in tissue engineering. By regulating related genes, it can inhibit chondrocyte apoptosis and reduce oxidative stress.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2021)
Article
Chemistry, Physical
Yujie Chen, Wei Xu, Muhammad Shafiq, Jincheng Tang, Junxiang Hao, Xianrui Xie, Zhengchao Yuan, Xianghao Xiao, Yu Liu, Xiumei Mo
Summary: This study successfully fabricated two-dimensional and three-dimensional electrospun poly(L-lactide-co-epsilon-caprolactone)/silk fibroin scaffolds with nanofibrous structure and biological function, further mimicking the microarchitecture of native cartilage through crosslinking. The three-dimensional scaffolds showed better mechanical properties, while the biocompatibility, chondrocyte proliferation, and phenotypic maintenance were superior in the three-dimensional scaffolds as well.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Materials Science, Multidisciplinary
Deyu Yang, Zhou Fang, Rui Kang, Kehai Liu
Summary: A new composite electrospun scaffold for pelvic organ prolapse showed promising potential in physicochemical characterizations and cell tests, indicating good biocompatibility and potential for pelvic floor regeneration.
MATERIALS & DESIGN
(2021)
Review
Materials Science, Biomaterials
Alex P. Rickel, Xiajun Deng, Daniel Engebretson, Zhongkui Hong
Summary: Due to the prevalence of cardiovascular diseases, there is a large demand for small diameter vascular grafts, with electrospinning being considered a promising approach. A successful tissue engineered vascular graft needs to match the mechanical properties of native tissue and possess characteristics that do not lead to thrombosis or immune response.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Materials Science, Multidisciplinary
Yuriy Snyder, Soumen Jana
Summary: Creating leaflet substrates that mimic the trilayered structure, anisotropic tensile characteristics, and elastomeric features of human heart valve leaflets is crucial to successful heart valve tissue engineering. In this study, elastomeric trilayer substrates made of a combination of polymers were developed to resemble native tissue. These substrates exhibited better cell adhesion, proliferation, extracellular matrix production, and resistance to calcification compared to non-elastomeric substrates.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Polymer Science
Siripanyo Promnil, Chaiwat Ruksakulpiwat, Piya-on Numpaisal, Yupaporn Ruksakulpiwat
Summary: Silk fibroin (SF) was used to improve cell adhesion and degradability of poly(lactic acid) (PLA) based scaffolds. By studying solution viscosity and SF content, a PLA/SF scaffold with both biological and mechanical properties was fabricated. The addition of SF improved the in vitro degradability and hydrophilicity of PLA based scaffolds, showing potential for meniscus tissue regeneration.
Article
Biochemistry & Molecular Biology
Fereshte Ghafari, Saeed Karbasi, Mohamadreza Baghaban Eslaminejad, Forough Azam Sayahpour, Niloofar Kalantari
Summary: This study investigated the impact of alumina nanowire on the physical and biological properties of a polyhydroxybutyratekeratin (PHB-K) electrospun scaffold. PHB-K/alumina nanowire nanocomposite scaffolds were made with an optimal concentration of 3 wt% alumina nanowire using the electrospinning method. The nanocomposite scaffold showed improved degradation rate, bioactivity, cell viability, and gene expression compared to other scaffolds, making it a potential construct for osteogenic induction in bone tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Mohammad Mohammadalipour, Tayebeh Behzad, Saeed Karbasi, Mojtaba Babaei Khorzoghi, Zahra Mohammadalipour
Summary: This study prepared a novel electrospun nanocomposite structure (PLC) by adding lignin and cellulose nanofiber to PHB, which showed more symmetrical fibers, higher surface roughness, better hydrophilicity, smaller crystal size, and more reasonable biodegradability compared to PHB. These changes led to the improvement of mechanical properties, cell behavior, bioactivity, and osteogenesis promotion, suggesting that PLC could enhance biological responses compared to PHB.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Maryam Zarei, Abdolreza Samimi, Mohammad Khorram, Mahnaz M. Abdi, Seyyed Iman Golestaneh
Summary: The conductive electrospun nanofiber scaffold containing polypyrrole was fabricated to accelerate tissue healing, with polypyrrole dispersed perfectly in the fibers and enhancing their conductivity up to 164.274 x 10(-3) s/m. The nanofibers with 10% polypyrrole showed better cell adhesion, growth, and proliferation properties, making them suitable for various tissue applications and particularly appropriate for body parts with electrical signals such as cardiovascular, heart muscles, or nerves.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Cardiac & Cardiovascular Systems
Yingfei Xue, Alexander P. Kossar, Alexey Abramov, Antonio Frasca, Mingze Sun, Mariya Zyablitskaya, David Paik, David Kalfa, Mila Della Barbera, Gaetano Thiene, Satoshi Kozaki, Takayuki Kawashima, Joseph H. Gorman III, Robert C. Gorman, Matthew J. Gillespie, Chrystalle Katte Carreon, Stephen P. Sanders, Robert J. Levy, Giovanni Ferrari
Summary: This study investigates the mechanisms of structural valve degeneration in young patients and juvenile animals with bioprosthetic heart valves, and presents models for studying this phenomenon.
CARDIOVASCULAR RESEARCH
(2023)
Article
Multidisciplinary Sciences
Andrey Zakharchenko, Yingfei Xue, Samuel Keeney, Christopher A. Rock, Ivan S. Alferiev, Stanley J. Stachelek, Hajime Takano, Tina Thomas, Chandrasekaran Nagaswami, Abba M. Krieger, Michael Chorny, Giovanni Ferrari, Robert J. Levy
Summary: This study shows that poly-2-methyl-2-oxazoline (POZ) modification of bovine pericardium reduces the accumulation of advanced glycation end products (AGE) and serum proteins in bioprosthetic heart valves (BHV), mitigating structural valve degeneration (SVD). POZ-modified BHV demonstrated reduced accumulation of serum albumin and AGE and maintained collagen microarchitecture. Ethanol pretreatment inhibited both AGE and calcification accumulation. This research suggests that POZ functionalization enhances biocompatibility and ethanol pretreatment can prevent calcification in BHV.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Jackson E. Powell, Colin K. W. Lim, Ramya Krishnan, Tristan X. McCallister, Christian Saporito-Magrina, Maria A. Zeballos, Garrett D. McPheron, Thomas Gaj
Summary: Cas13 nucleases are programmable RNA-targeting CRISPR effector proteins that can silence target gene expression in mammalian cells. By delivering RfxCas13d to the mouse spinal cord and brain, neurodegeneration-associated genes can be effectively silenced, offering a versatile platform for gene knockdown in the nervous system.
Article
Multidisciplinary Sciences
Anna Daily, Prashanth Ravishankar, Steve Harms, V. Suzanne Klimberg
Summary: This study identified protein biomarkers with altered expression levels in tear samples from women diagnosed with breast cancer. Through LC-MS/MS analysis, 14 potential biomarkers were identified and validated by ELISA. The results showed significantly higher expression of S100A8 and S100A9 in breast cancer patients, while Galectin-3 binding protein had increased expression in the control group. These findings support the use of tear proteins in detecting systemic diseases such as breast cancer.
Article
Cell & Tissue Engineering
Srikanth Sivaraman, Prashanth Ravishankar, Raj R. Rao
Summary: Cardiovascular diseases are a leading cause of death and disability worldwide. Stem cells offer potential for regenerating smooth muscle tissue, but effective myogenic differentiation requires careful assessment of stem cell characteristics, culture conditions, and microenvironments.
TISSUE ENGINEERING PART B-REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Prashanth Ravishankar, Anna Daily
Summary: This study compared the genomic and proteomic profiles of tears and blood, revealing similarities and differences in protein and microRNA expression. Tears may serve as an alternative biofluid to blood.
APPLIED SCIENCES-BASEL
(2022)
Article
Cardiac & Cardiovascular Systems
Prashanth Ravishankar, Ishita Tandon, Kartik Balachandran
Summary: This study focused on examining the role of uniaxial cyclic strain on endothelial progenitor cells cultured on 3D composites mimicking aortic valve tissue. The experiment revealed that cyclic strain led to changes in cell phenotype and altered expression of related markers.
CARDIOVASCULAR ENGINEERING AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Colin K. W. Lim, Tristan X. McCallister, Christian Saporito-Magrina, Garrett D. McPheron, Ramya Krishnan, M. Alejandra Zeballos, Jackson E. Powell, Lindsay Clark, Pablo Perez-Pinera, Thomas Gaj
Summary: The study demonstrates the broad application potential of CRISPR base editing technology in modulating gene expression through editing non-coding regulatory elements. It shows the specific effectiveness of base editors in perturbing gene expression.
Article
Engineering, Biomedical
Mingze Sun, Mohamed Elkhodiry, Lei Shi, Yingfei Xue, Maryam H. Abyaneh, Alexander P. Kossar, Caroline Giuglaris, Samuel L. Carter, Richard L. Li, Emile Bacha, Giovanni Ferrari, Jeffrey Kysar, Kristin Myers, David Kalfa
Summary: Currently used materials for heart valve repair or replacement are not durable due to inadequate mechanical properties and biocompatibility. This study developed a biomimetic, multilayered material (BMM) that mimics the structure and properties of native valve leaflets to improve durability.
Review
Biotechnology & Applied Microbiology
Zhong Alan Li, Shilpa Sant, Sung Kwon Cho, Stuart B. Goodman, Bruce A. Bunnell, Rocky S. Tuan, Michael S. Gold, Hang Lin
Summary: Synovial joint disorders like osteoarthritis and rheumatoid arthritis are common worldwide, but current clinical management does not fully restore joint function. Organ-on-chip (OoC) technology, which can culture multiple human cell-derived tissues/organs, shows potential in developing better therapies for joint diseases.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Alexey Abramov, Yingfei Xue, Andrey Zakharchenko, Mangesh Kurade, Rajesh K. Soni, Robert J. Levy, Giovanni Ferrari
Summary: A study found that metabolic syndrome is a risk factor for heart valve degeneration, and patients with metabolic syndrome require valve replacement earlier. The study used advanced proteomics to identify significant differences in the protein composition of heart valves between patients with and without metabolic syndrome. Furthermore, the study demonstrated that a protective modification can mitigate the degeneration of heart valve biomaterial caused by metabolic syndrome.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Editorial Material
Biotechnology & Applied Microbiology
Yingfei Xue, Payam Akhyari, Bin Duan, Hooi Hooi Ng, Zongmin Zhao
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Akhil Patel, Jessica Kosanovich, Sameera Sansare, Sricharani Balmuri, Vinayak Sant, Kerry M. Empey, Shilpa Sant
Summary: This study investigates the potential of cube and sphere-shaped cerium oxide nanoparticles (CNP) to modulate reactive oxygen (ROS) and nitrogen (RNS) species and immune cell phenotypes in the presence of RSV infection. The results show that cube CNP significantly reduces RSV-induced ROS levels and drives an M1 phenotype in RSV-infected macrophages. Sphere CNP increases RSV-induced RNS levels with minimal effect on ROS levels and decreases M2 marker CD206 expression. Cube CNP preferentially accumulates in murine alveolar macrophages and induces their activation, avoiding enhanced uptake and activation of other inflammatory cells.
BIOACTIVE MATERIALS
(2023)
Article
Medicine, Research & Experimental
Paola Monterroso Diaz, Ashton Leehans, Prashanth Ravishankar, Anna Daily
Summary: Cancer is a complex and heterogeneous disease, and liquid biopsies have emerged as a promising non-invasive method for cancer detection and monitoring. The integration of -omics technologies has further enhanced the capabilities of liquid biopsies, enabling personalized treatment based on individual tumor biology.
BIOMARKER INSIGHTS
(2023)
Meeting Abstract
Cell & Tissue Engineering
I. Tandon, C. Gish, N. Sturdivant, A. Ozkizilcik, C. Dunn, P. Ravishankar, A. Walls, L. Ferreira, K. Balachandran
TISSUE ENGINEERING PART A
(2022)
Article
Polymer Science
Zhongming Yuan, Hiroko Yamamoto, Shinji Kanehashi, Takeshi Shimomura
Summary: This study reports the synthesis of a flexible light-emitting polymer film using a solution blending method. The selective positioning of MEH-PPV molecules in the PS phase of the film was confirmed. The film exhibited a nanometer-scale luminescent domain with low aggregation quenching and showed good elastomeric characteristics.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2024)