Preparation of multitarget Fusarium toxin (zearalenone, deoxynivalenol, T-2, and HT-2) immunoaffinity columns using poly(glycidyl methacrylate-divinylbenzene) as a matrix

This study aimed to establish a purifying immunoaffinity column (IAC) for four kinds of Fusarium toxins (zearalenone, deoxynivalenol, T-2, and HT-2) simultaneously using poly-glycidyl methacrylate-divinylbenzene [P(GMA-DVB)] as the column matrix. The P(GMA-DVB) microsphere was synthesized using glycidyl methacrylate (GMA) and diethylbenzene (DVB) at an optimal polymerization temperature of 80 degrees C, the initiator 2,2 '-azodiisobutyronitrile concentration of 2.50 g/mL H2O, and the DVB-to-GMA ratio of 3:5 (wt.). The polymerized P(GMA-DVB) microspheres were modified with 1,6-hexanediamine, activated with glutaraldehyde, and coupled with the four toxin monoclonal antibodies. The coupling rate of the activated resin was calculated to be 95.95%. The maximum capacity of the IACs was 264 ng deoxynivalenol, 231 ng T-2, 210 ng HT-2, and 292 ng zearalenone. After eight uses, the maximum capacity had no obvious decrease. The recovery of the four Fusarium toxins on the P(GMA-DVB) IAC was 70.03-85.39% in flour, with the relative standard deviations of 4.67-15.93%. The IACs were applied to the actual flour samples.