Journal
PLANT SCIENCE
Volume 287, Issue -, Pages -Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2019.110184
Keywords
Cold stress; beta-amylase (BAM); Osmolyte; Starch degradation; Antioxidant enzyme; Reactive oxygen species (ROS)
Categories
Funding
- Excellent Youth Natural Science Foundation of Jiangsu Province [BK20170086]
- open funds of the State Key Laboratory of Crop Genetics and Germplasm Enhancement [ZW201908]
- National Key Research and Development Program of China [2018YFD1000300]
- National Science Foundation of China [31872070]
- Jiangsu Agriculture Science and Technology Innovation Fund [CX(18)3065]
- Fundamental Research Funds for the Central Universities [KYZ201607]
- National Natural Science Foundation of China [31800584]
- Anhui Provincial Natural Science Foundation [1808085QC75]
- Anhui Provincial Postdoctoral Science Foundation [2017B158]
- Science Foundation for Anhui Province [KJ2018A0129]
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beta-Amylase (BAM) is involved in sugar metabolism, but the role of BAM genes in cold tolerance remains poorly understood. Here, we report the identification and functional characterization of the chloroplast-localized BAM-encoding gene PbrBAM3 isolated from Pyrus betulaefolia. The transcript levels of PbrBAM3 were up-regulated under cold, dehydration and ABA, but repressed by maltose. Overexpression of PbrBAM3 in tobacco (Nicotiana tabacum) and pear (P. ussuriensis) conferred increased BAM activity, promoted starch degradation after chilling treatments and enhanced tolerance to cold. Under the chilling stress, the transgenic tobacco and P. ussuriensis exhibited lessened reactive oxygen species (ROS) generation, higher levels of antioxidant enzymes activity, and greater accumulation of soluble sugars (specially maltose) than the corresponding wild type plants. Taken together, these results demonstrate that PbrBAM3 plays an important role in cold tolerance, at least in part, by raising the levels of soluble sugars capable of acting as osmolytes or antioxidants.
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