Article
Biochemistry & Molecular Biology
Gang Wang, Yongyan Wang, Jiacheng Ni, Rongrong Li, Fengling Zhu, Ruyin Wang, Qiuzhen Tian, Qingwen Shen, Qinghua Yang, Jihua Tang, Monika W. Murcha, Guifeng Wang
Summary: The CRK1 gene in maize encodes a crucial assembly factor for mitochondrial complex I, which is essential for both the assembly of complex I and seed development in plants. The study also reveals the interaction between CRK1 and two other proteins, leading to their involvement in complex I assembly.
Article
Biochemistry & Molecular Biology
Liang Wang, Taru Hilander, Xiaonan Liu, Hoi Ying Tsang, Ove Eriksson, Christopher B. Jackson, Markku Varjosalo, Hongxia Zhao
Summary: This study identifies GTPBP8 as a crucial factor in the assembly of the mitoribosomal large subunit (mt-LSU) and mitochondrial translation. Depletion of GTPBP8 leads to abnormal accumulation of mt-LSU and compromised mitochondrial function.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cell Biology
C. Dilanka Fernando, W. Samantha N. Jayasekara, Chaitanya Inampudi, Maija R. J. Kohonen-Corish, Wendy A. Cooper, Traude H. Beilharz, Tracy M. Josephs, Daniel J. Garama, Daniel J. Gough
Summary: This study identifies a complex consisting of STAT3, LRPPRC, and SLIRP, which is required for the stability and transport of mature mito-chondrially encoded mRNAs to the mitochondrial ribosome. The complex is enriched in patients with lung adenocarcinoma and its deletion inhibits the growth of lung cancer.
Article
Cell Biology
Hezhi Fang, Anran Xie, Miaomiao Du, Xueyun Li, Kaiqiang Yang, Yinxu Fu, Xiangshu Yuan, Runxiao Fan, Weidong Yu, Zhuohua Zhou, Tiantian Sang, Ke Nie, Jin Li, Qiongya Zhao, Zhehui Chen, Yanling Yang, Chaoyang Hong, Jianxin Lyu
Summary: The study revealed that MEGD(H)EL syndrome shares clinical and molecular features with mtDNA depletion syndrome, which is mainly caused by insufficient supply of nucleotides. The supplementation of nucleosides/nucleotides may be an effective therapeutic strategy for MEGD(H)EL syndrome, as it can restore mtDNA content and mitochondrial function.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Luke E. Formosa, Boris Reljic, Alice J. Sharpe, Daniella H. Hock, Linden Muellner-Wong, David A. Stroud, Michael T. Ryan
Summary: This study shows that the TMEM126A protein is involved in the assembly of mitochondrial complex I, specifically with the ND4 subunit, and its function is distinct from its paralogue TMEM126B. Mutations in TMEM126A lead to an isolated deficiency in complex I.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Jonathan Tai, Rachel M. Guerra, Sean W. Rogers, Zixiang Fang, Laura K. Muehlbauer, Evgenia Shishkova, Katherine A. Overmyer, Joshua J. Coon, David J. Pagliarini
Summary: The study reveals that Hem25p, a mitochondrial glycine transporter required for haem biosynthesis, also acts as an isopentenyl pyrophosphate (IPP) transporter in Saccharomyces cerevisiae, playing a crucial role in coenzyme Q synthesis.
NATURE CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Nils Burger, Andrew M. James, John F. Mulvey, Kurt Hoogewijs, Shujing Ding, Ian M. Fearnley, Marta Loureiro-Lopez, Abigail A. Norman, Sabine Arndt, Amin Mottahedin, Olga Sauchanka, Richard C. Hartley, Thomas Krieg, Michael P. Murphy
Summary: This study found that Cys39 of ND3 is exposed in complex I during mitochondrial respiration, which has significant implications for our understanding of the A/D transition and the mechanism of complex I.
CELL CHEMICAL BIOLOGY
(2022)
Article
Plant Sciences
Yong Wang, Xin-Yuan Liu, Zi-Qin Huang, Yan-Yan Li, Yan-Zhuo Yang, Aqib Sayyed, Feng Sun, Zhi-Qun Gu, Xiaomin Wang, Bao-Cai Tan
Summary: The study revealed the crucial role of EMP17 in C-to-U editing at multiple sites in mitochondrial transcripts, complex III biogenesis, and seed development in maize.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
You Li, Ichiro Misumi, Tomoyuki Shiota, Lu Sun, Erik M. Lenarcic, Hyejeong Kim, Takayoshi Shirasaki, Adriana Hertel-Wulff, Taylor Tibbs, Joseph E. Mitchell, Kevin L. McKnight, Craig E. Cameron, Nathaniel J. Moorman, David R. McGivern, John M. Cullen, Jason K. Whitmire, Stanley M. Lemon
Summary: Despite the availability of effective vaccines, recent outbreaks of hepatitis A in the United States have led to numerous hospitalizations and deaths. This study reveals the importance of ZCCHC14 and TENT4A/B in viral RNA synthesis and suggests that inhibitors of TENT4A/B could be useful in preventing or treating hepatitis A in humans.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Ryo Tsukimoto, Kazuho Isono, Takuma Kajino, Satoshi Iuchi, Akihisa Shinozawa, Izumi Yotsui, Yoichi Sakata, Teruaki Taji
Summary: This study identified the SLOH5 gene and revealed the importance of mitochondrial fission and function in long-term heat tolerance in Arabidopsis.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Helene Roehricht, Jonathan Przybyla-Toscano, Joachim Forner, Clement Boussardon, Olivier Keech, Nicolas Rouhier, Etienne H. Meyer
Summary: This study identified an atypical mitochondrial ferredoxin (mFDX-like) that plays a crucial role in the assembly of complex I and formation of complex I-containing supercomplexes.
Article
Biochemistry & Molecular Biology
Yeon-Ji Park, Niranjan Dodantenna, Yonghyeon Kim, Tae-Hwan Kim, Ho-Soo Lee, Young-Suk Yoo, June Heo, Jae-Ho Lee, Myung-Hee Kwon, Ho Chul Kang, Jong-Soo Lee, Hyeseong Cho
Summary: The NLRP3 inflammasome is activated through mitochondria-mediated ubiquitination by the E3 ligase MARCH5. Knockout of MARCH5 leads to an impaired secretion of IL-1β and IL-18 in myeloid cells, as well as a decreased mortality rate upon infection. MARCH5-dependent NLRP3 ubiquitination is essential for NLRP3-NEK7 complex formation and NLRP3 oligomerization.
Article
Multidisciplinary Sciences
Thomas D. Jackson, Jordan J. Crameri, Linden Muellner-Wong, Ann E. Frazier, Catherine S. Palmer, Luke E. Formosa, Daniella H. Hock, Kenji M. Fujihara, Tegan Stait, Alice J. Sharpe, David R. Thorburn, Michael T. Ryan, David A. Stroud, Diana Stojanovski
Summary: Sideroflexins (SFXNs) are a family of proteins that have different functions in mitochondrial biology. Loss of SFXN4 leads to complex I assembly defect and it interacts with the core components of the mitochondrial complex I intermediate assembly complex.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Pharmacology & Pharmacy
Eugenia Trushina, Sergey Trushin, Md Fayad Hasan
Summary: Alzheimer's disease, the most common form of dementia in the elderly, has no cure. Emerging data suggest that mitochondria can initiate an integrated stress response that is beneficial for healthy aging and neuroprotection. Partial inhibition of mitochondrial complex I has been explored as a treatment strategy for various diseases, including Alzheimer's disease.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Multidisciplinary Sciences
Enrique Balderas, David R. Eberhardt, Sandra Lee, John M. Pleinis, Salah Sommakia, Anthony M. Balynas, Xue Yin, Mitchell C. Parker, Colin T. Maguire, Scott Cho, Marta W. Szulik, Anna Bakhtina, Ryan D. Bia, Marisa W. Friederich, Timothy M. Locke, Johan L. K. Van Hove, Stavros G. Drakos, Yasemin Sancak, Martin Tristani-Firouzi, Sarah Franklin, Aylin R. Rodan, Dipayan Chaudhuri
Summary: Mitochondrial complex I deficiency is common in congenital, neurologic, and cardiovascular diseases. This study reveals that Complex I stimulates the turnover of a mitochondrial calcium channel, preserving energetic homeostasis during Complex I deficiency. Calcium influx into mitochondria enhances ATP synthesis and is increased in cardiomyopathies caused by mitochondrial dysfunction. The impairment of Complex I leads to increased activity of the mitochondrial calcium uniporter channel. The authors propose that calcium influx compensates for Complex I dysfunction via an impairment in Complex I-induced protein turnover and Uniporter stabilization.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Abi S. Ghifari, Pedro F. Teixeira, Beata Kmiec, Neha Singh, Elzbieta Glaser, Monika W. Murcha
Summary: In this study, the researchers identified a prolyl aminopeptidase homologue, PAP1, which has two isoforms targeting different subcellular locations. They found that PAP1 may play a role in proline homeostasis and pollen development in plants. The results suggest that PAP1 is involved in the chloroplastic peptide processing pathway and could be important for maintaining cellular stability and activity.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Andreas Hartmann, Oliver Berkowitz, James Whelan, Reena Narsai
Summary: This study analyzed the transcriptomic responses of Arabidopsis, barley, and rice to different treatments, and compared the similarities and differences between species. The results showed that the number of differentially expressed genes varied between treatments and species, but at least 70% of these genes overlapped within a species, indicating overlapping response networks. Interestingly, some orthologous genes exhibited opposite responses between species, suggesting diversity in their responses. The orthologous genes that showed a common response across the three species were found to be functionally important in stress responses. The study also revealed the high conservation of the mitochondrial dysfunction response in all three species.
Article
Plant Sciences
Ying Li, Katharina Belt, Saad F. Alqahtani, Saurabh Saha, Ricarda Fenske, Olivier Van Aken, James Whelan, A. Harvey Millar, Monika W. Murcha, Shaobai Huang
Summary: This study reveals the critical role of SDHAF1 in maintaining glycolysis and mitochondrial respiration. SDHAF1 participates in the biogenesis of complex II by interacting with SDH2 and HSCB.
Article
Plant Sciences
Crystal Sweetman, Jennifer Selinski, Troy K. Miller, James Whelan, David A. Day
Summary: Alternative oxidase (AOX) is an important component of the plant respiratory pathway, allowing electrons to bypass certain complexes in the mitochondrial electron transport chain. AOX has multiple isoforms, with AOX1 receiving more attention due to its importance in stress responses and AOX2 being highly accumulated in legume photosynthetic tissues. Differential activation kinetics with pyruvate were observed between AOX1 and AOX2 isoforms, suggesting that selective expression of these isoforms might determine the level of AOX activity. However, this cannot fully explain the differences seen in sub-mitochondrial particles isolated from different legume tissues.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Wujian Wang, Jun Ye, Heng Xu, Xi Liu, Yue Fu, Hui Zhang, Hatem Rouached, James Whelan, Zhenguo Shen, Luqing Zheng
Summary: The study reveals that the bHLH transcription factor OsbHLH061 interacts with OsPRI1 to regulate iron homeostasis in rice, ensuring plants adapt to varying iron environments and maintain iron balance. OsbHLH061 knockout or overexpression lines alter iron accumulation in shoots, and the OsbHLH061-OsPRI1 complex recruits co-repressors to repress the expression of iron-related transcription factors OsIRO2 and OsIRO3.
Article
Plant Sciences
Yue Qu, Rongxia Guan, Lili Yu, Oliver Berkowitz, Rakesh David, James Whelan, Melanie Ford, Stefanie Wege, Lijuan Qiu, Matthew Gilliham
Summary: GmSALT3 is a major gene determining salt tolerance in soybean, which improves the ability of soybean plants to cope with saline stress through regulation of root response. Genes related to oxidation reduction, calcium signaling, vesicle trafficking, and diffusion barrier formation are upregulated in salt-tolerant soybean lines.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Plant Sciences
Runze Guo, Qi Zhang, Yinghui Ying, Wenying Liao, Yu Liu, James Whelan, Mao Chuanzao, Huixia Shou
Summary: This study found that SPX-MFS genes in rice vacuoles are Pi influx transporters, with OsSPX-MFS3 playing the most important role in growth, vacuolar, and tissue Pi concentrations. Alteration of vacuolar Pi sequestration may improve rice tolerance to low phosphorus.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Plant Sciences
Stephanie J. Watts-Williams, Stefanie Wege, Sunita A. Ramesh, Oliver Berkowitz, Bo Xu, Matthew Gilliham, James Whelan, Stephen D. Tyerman
Summary: Soil micronutrient availability, particularly zinc (Zn), is a limiting factor in crop yield. Arbuscular mycorrhizal (AM) fungi facilitate nutrient uptake in host plants through the mycorrhizal pathway. In this study, RNA-seq analysis revealed that the putative Zn transporter gene, MtZIP14, was up-regulated in Medicago truncatula roots during colonization by Rhizophagus irregularis. Loss-of-function mutant plants lacking MtZIP14 showed reduced shoot biomass when grown under low Zn concentration and colonized by AM fungi, implicating a role for MtZIP14 in plant Zn nutrition and AM colonization.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biochemistry & Molecular Biology
Yanqiao Zhu, Reena Narsai, Cunman He, Yan Wang, Oliver Berkowitz, James Whelan, Lim Chee Liew
Summary: Mitochondrial retrograde signaling (MRS) regulates photosynthetic function under different conditions by controlling the binding of ANAC017 to promoters and the expression of related transcription factors. The study also found that there is a separate MRS pathway stimulated by antimycin A treatment, which intersects with salicylic acid signaling and chloroplast retrograde signaling. Additionally, the transcriptional responses to antimycin A and myxothiazol in the dark are ANAC017 independent and involve ERF and ZAT transcription factors, resembling biotic challenge and salicylic acid responses.
PLANT COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Marta Peirats-Llobet, Changyu Yi, Lim Chee Liew, Oliver Berkowitz, Reena Narsai, Mathew G. Lewsey, James Whelan
Summary: A spatial transcriptomics workflow was developed for studying gene expression during barley grain germination, revealing specific spatial expression patterns of functional gene categories. Over 14,000 genes were differentially regulated within the first 24 hours after imbibition, with certain genes showing focused expression within subdomains of the embryo over time, suggesting their role in establishing the embryo axis.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Abi S. Ghifari, Aneta Ivanova, Oliver Berkowitz, James Whelan, Monika W. Murcha
Summary: This study describes the mechanism by which the abundance of Complex I (CI) is regulated in a CI-deficient Arabidopsis thaliana mutant. The interaction between the CI subunit PSST and FTSH3 facilitates the disassembly of the CI matrix arm domain for turnover, ensuring protein quality control in the face of oxidative damage. The study reveals the molecular process by which FTSH3 recognizes CI for degradation at the amino acid level.
Article
Plant Sciences
Abi S. Ghifari, Saurabh Saha, Monika W. Murcha
Summary: Mitochondria are crucial for plant respiration, and their oxidative phosphorylation (OXPHOS) system generates ATP for cellular energy needs. OXPHOS complexes consist of subunits from both mitochondrial and nuclear genomes, requiring careful regulation of expression, translation, import, and assembly. OXPHOS subunits are prone to oxidative damage due to reactive oxygen species, necessitating coordinated disassembly and degradation. This review highlights recent studies on the composition, assembly, and activity of plant OXPHOS complexes, and discusses the regulation of their biogenesis and turnover, as well as the importance of OXPHOS in plant respiration and growth.
Article
Plant Sciences
Pavani P. Nadiminti, Sarah M. Wilson, Allison van de Meene, Alfie Hao, John Humphries, Julian Ratcliffe, Changyu Yi, Marta Peirats-Llobet, Mathew G. Lewsey, James Whelan, Antony Bacic, Monika S. Doblin
Summary: This study analyzed the deposition pattern of MLG during oat endosperm development and revealed the complexity of molecular processes influencing MLG content and deposition.
