An optimised CRISPR/Cas9 protocol to create targeted mutations in homoeologous genes and an efficient genotyping protocol to identify edited events in wheat

Versatile and multifaceted CRISPR/Cas gene editing tool for plant research

(2019) Prashant K. Pandey et al.   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

Optimized paired-sgRNA/Cas9 cloning and expression cassette triggers high-efficiency multiplex genome editing in kiwifruit

(2018) Zupeng Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single-cell mutation detection to mutant plant regeneration

(2018) Choun-Sea Lin et al.   PLANT BIOTECHNOLOGY JOURNAL

A simple and cost-effective method for screening of CRISPR/Cas9-induced homozygous/biallelic mutants

(2018) Jinggong Guo et al.   Plant Methods

Concurrent modifications in the three homeologs of Ms45 gene with CRISPR-Cas9 lead to rapid generation of male sterile bread wheat (Triticum aestivum L.)

(2018) Manjit Singh et al.   PLANT MOLECULAR BIOLOGY

Shifting the limits in wheat research and breeding using a fully annotated reference genome

(2018) et al.   SCIENCE

Efficient generation of stable, heritable gene edits in wheat using CRISPR/Cas9

(2018) Rhian M Howells et al.   BMC PLANT BIOLOGY

Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat

(2017) Yunwei Zhang et al.   PLANT JOURNAL

Intron-Mediated Enhancement: A Tool for Heterologous Gene Expression in Plants?

(2017) Miriam Laxa   Frontiers in Plant Science

Cas-analyzer: an online tool for assessing genome editing results using NGS data

(2016) Jeongbin Park et al.   BIOINFORMATICS

Efficient Targeted Genome Modification in Maize Using CRISPR/Cas9 System

(2016) Chao Feng et al.   Journal of Genetics and Genomics

Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

(2016) Yi Zhang et al.   Nature Communications

Systematic analysis of CRISPR–Cas9 mismatch tolerance reveals low levels of off-target activity

(2015) Emily M. Anderson et al.   JOURNAL OF BIOTECHNOLOGY

In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing

(2015) Y. Liu et al.   mBio

Genome editing in rice and wheat using the CRISPR/Cas system

(2014) Qiwei Shan et al.   Nature Protocols

Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice

(2014) Huanbin Zhou et al.   NUCLEIC ACIDS RESEARCH

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Targeted mutagenesis in rice using CRISPR-Cas system

(2013) Jin Miao et al.   CELL RESEARCH

Targeted genome modification of crop plants using a CRISPR-Cas system

(2013) Qiwei Shan et al.   NATURE BIOTECHNOLOGY

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice

(2013) Wenzhi Jiang et al.   NUCLEIC ACIDS RESEARCH

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

CRISPR-Cas Systems in Bacteria and Archaea: Versatile Small RNAs for Adaptive Defense and Regulation

(2011) Devaki Bhaya et al.   Annual Review of Genetics

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS