Journal
PLANT BREEDING
Volume 139, Issue 2, Pages 375-380Publisher
WILEY
DOI: 10.1111/pbr.12772
Keywords
broad bean; candidate gene; flower colour; molecular breeding; SNP marker; tannins
Funding
- ADF (Agriculture Development Fund), Ministry of Agriculture, Government of Saskatchewan, Canada
- SPG (Saskatchewan Pulse Growers), Saskatoon, Canada
- Western Grains Research Foundation, Canada
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Faba bean has the potential to become a key food and feed protein crop in many areas of the world. The presence of tannins in its seed coat has limited the deployment of this crop as feed and food. The expression of either of the two recessive genes, zt1 and zt2, causes a great reduction of tannins from the seed coat and results in a white flower phenotype. Molecular markers linked to these loci are fundamental tools for speeding up the breeding of low-tannin varieties. The main aim of this study was to develop and validate a robust molecular marker linked to the zt2 locus. We used 176 recombinant inbred lines of the Disco/2 x ILB 938/2 cross at F-6 and genotyped those using 257 SNP (single nucleotide polymorphism) markers. An SNP marker associated with zt2 locus was found on faba bean chromosome 3 and was used to develop a high-throughput low-cost KASP (kompetitive allele-specific PCR) marker. The KASP marker can successfully discriminate low-tannin faba beans carrying zt2 from those carrying zt1 and wild-type alleles.
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