4.8 Article

Boosting dark fermentation with co-cultures of extreme thermophiles for biohythane production from garden waste

Journal

BIORESOURCE TECHNOLOGY
Volume 219, Issue -, Pages 132-138

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.07.096

Keywords

Thermotoga maritima; Caldicellulosiruptor saccharolyticus; Caldicellulosiruptor bescii; Co-cultures; Biohythane; Garden waste

Funding

  1. Portuguese Science Foundation (FCT)
  2. European Social Fund (ESF, POPH-QREN) [SFRH/BPD/82000/2011]
  3. FCT Strategic Project of unit [UID/BIO/04469/2013]
  4. COMPETE [POCI-01-0145-FEDER-006684, RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462)]
  5. Fundação para a Ciência e a Tecnologia [RECI/BBB-EBI/0179/2012] Funding Source: FCT

Ask authors/readers for more resources

Proof of principle of biohythane and potential energy production from garden waste (GW) is demonstrated in this study in a two-step process coupling dark fermentation and anaerobic digestion. The synergistic effect of using co-cultures of extreme thermophiles to intensify biohydrogen dark fermentation is demonstrated using xylose, cellobiose and GW. Co-culture of Caldicellulosiruptor saccharolyticus and Thermotoga maritima showed higher hydrogen production yields from xylose (2.7 +/- 0.1 mol mol(-1) total sugar) and cellobiose (4.8 +/- 0.3 mol mol(-1) total sugar) compared to individual cultures. Co-culture of extreme thermophiles C. saccharolyticus and Caldicellulosiruptor bescii increased synergistically the hydrogen production yield from GW (98.3 +/- 6.9 L kg(-1) (VS)) compared to individual cultures and co-culture of T. maritima and C. saccharolyticus. The biochemical methane potential of the fermentation end-products was 322 +/- 10 L kg(-1) (CODt). Biohythane, a biogas enriched with 15% hydrogen could be obtained from GW, yielding a potential energy generation of 22.2 MJ kg(-1) (VS). (C) 2016 Elsevier Ltd. All rights reserved.

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