Journal
BIORESOURCE TECHNOLOGY
Volume 213, Issue -, Pages 64-68Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.01.133
Keywords
Lipstatin; Pancreatic lipase inhibitor; Acyl CoA carboxylase; Fatty acid metabolism; Submerged fermentation
Funding
- SERB-DST [SR/FT/LS-45/2012]
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This work investigated the potential of medium engineering to obtain maximum biomass, non-conventional carbon sources for lipstatin production and modulation of tricarboxylic acid (TCA) cycle to promote lipstatin synthesis. It was found that 2: 3 carbon and nitrogen ratio, produced maximum biomass of 7.9 g/L in growth medium and 6.6 g/L in pre-seed medium. Among the studied non-conventional carbon sources i.e., soya flour 40 g/L and sesame oil 30 mL/L were found producing 1109.37 mg/L (1.24-fold of control) and 1196.75 mg/L (1.34-fold of control) lipstatin respectively. Supplementation of TCA cycle intermediates revealed that NADH and succinic acid showed lipstatin production to 1132.99 mg/L and 1171.10 mg/L respectively. Experimental outcome was validated in 7 L bioreactor and produced 2242.63 mg/L lipstatin which was similar to 14% higher than shake flask. (C) 2016 Elsevier Ltd. All rights reserved.
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