Article
Biotechnology & Applied Microbiology
Augchararat Klongklaew, Kridsada Unban, Dharman Kalaimurugan, Apinun Kanpiengjai, Hassan Azaizeh, Linda Schroedter, Roland Schneider, Joachim Venus, Chartchai Khanongnuch
Summary: This study demonstrated the bioconversion of lignocellulosic by-product corn stover to L-lactic acid using specific strains of bacteria. The optimal conditions for the highest sugar release and L-LA production were determined. The purification of L-LA from the culture broth was successfully achieved. This study not only presents a feasible process for L-LA production from corn stover but also provides an alternative approach for solving the problem of haze air pollution caused by improper management of corn production residuals.
FERMENTATION-BASEL
(2023)
Article
Food Science & Technology
G. A. Dominguez-Gutierrez, I. Perraud-Gaime, H. Escalona-Buendia, N. Durand, E. I. Champion-Martinez, R. R. Fernandez-Martinez, G. Saucedo-Castaneda, G. Rodriguez-Serrano
Summary: This study optimized an inexpensive culture medium for Lactiplantibacillus plantarum MZ809351 strain B31, which resulted in a biomass concentration similar to the reference LAB culture medium. Sodium acetate and magnesium sulfate were the most significant factors affecting biomass concentration. Additionally, the inhibitory effects of LAB strains on the growth and production of OTA in a fungus were tested, and it was found that LAB can be used as a biological control agent against ochratoxigenic fungi in food.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
May Foong Ooi, Hooi Ling Foo, Teck Chwen Loh, Rosfarizan Mohamad, Raha Abdul Rahim, Arbakariya Ariff
Summary: The study successfully enhanced the antimicrobial activity of Postbiotic RS5 by improving culture density and reducing the cost of growth medium. This led to a significant reduction in production cost and improved cost-effectiveness, making Postbiotic RS5 a more feasible feed supplement for various livestock industries.
SCIENTIFIC REPORTS
(2021)
Article
Food Science & Technology
Caiqing Yao, Jing Li, Jingjing E, Ruixue Wang, Qiaoling Zhang, Junguo Wang
Summary: This study investigated the biofilm production and growth relationship of co-cultured lactic acid bacteria. The results showed that co-cultured lactobacilli strains promoted the growth of Streptococcus thermophilus, and vice versa. However, the formation of biofilm during co-cultivation was not necessarily related to symbiosis among the strains.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Jiang Jikang, Li Wenxiang, Yu Shuping
Summary: This study investigated the effects of inoculating different bacteria on the metabolite changes in fermented plant extract. The physicochemical and metabolite features of the treated extract were compared to those of spontaneously-fermented extract. The results showed that the physicochemical features of the extract fermented with inoculated bacteria were superior. Metabolite analysis revealed significant differences between the spontaneously-fermented group and the group inoculated with bacteria, including sugars, acids, and alcohols.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Menzi P. Ngwenya, Thabile P. Nkambule, Solomon W. Kidane
Summary: The aim of this study was to test the acceptability and physico-chemical characteristics of marula wine fermented with known cultures of natural Lactiplantibacillus plantarum and Saccharomyces cerevisiae. The results showed that Lactobacillus and Saccharomyces dominated the fermentation process and their individual cultures did not significantly affect the physico-chemical characteristics and acceptability of the wine. The wine fermented by Lactiplantibacillus plantarum alone was preferred due to its sweetness.
Review
Agricultural Engineering
Octavio Garcia-Depraect, Roberto Castro-Munoz, Raill Munoz, Eldon R. Rene, Elizabeth Leon-Becerril, Idania Valdez-Vazquez, Gopalakrishnan Kumar, Luis C. Reyes-Alvarado, Leonardo J. Martinez-Mendoza, Julian Carrillo-Reyes, German Buitron
Summary: Dark fermentation is a promising biological method to produce bio-hydrogen and other value added bio-products, but faces challenges like process instability and low hydrogen production yields. Inhibitory activity of lactate-producing bacteria against hydrogen producers has been highlighted as a major bottleneck, although lactate based metabolic pathways are considered prevalent in hydrogen production.
BIORESOURCE TECHNOLOGY
(2021)
Article
Cell & Tissue Engineering
Kohei Nagasaki, Ayumu Nakashima, Ryo Tamura, Naoki Ishiuchi, Kiyomasa Honda, Toshinori Ueno, Shigehiro Doi, Yukio Kato, Takao Masaki
Summary: This study compared the effects of serum-free cultured MSCs with MSCs cultured in medium containing fetal bovine serum on peritoneal fibrosis. The results showed that serum-free MSCs significantly suppressed fibrosis progression and inflammation, indicating their potential as a therapy for preventing peritoneal fibrotic progression. The in vitro experiments showed that although both types of MSCs suppressed TGF-beta 1 signaling, there was no significant difference between the two in their ability to do so.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Christopher H. Buhlmann, Bede S. S. Mickan, Stephan Tait, Damien J. J. Batstone, Parisa A. A. Bahri
Summary: Supplementing nitrogen and sucrose in food waste fermentation can improve lactic acid production. Both ammonium chloride and digestate showed similar improvements in the rate of lactic acid formation, but ammonium chloride also increased the final concentration. Digestate changed the microbial community composition and increased diversity, while sucrose minimized interference with lactic acid production and promoted Lactobacillus growth, leading to higher lactic acid concentrations. Overall, the study highlighted the value of digestate as a nutrient source and sucrose as a means to control microbial communities and enhance lactic acid production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Emergency Medicine
Kemal Sener, Banu Arslan, Sultan Ozselcuk, Ramazan Guven
Summary: Lactic acid is the end-product of anaerobic glycolysis and elevated blood lactate levels are associated with poor patient outcomes. While lactic acidosis can be caused by supplementary food intake in pediatric patients, it is uncommon in adult patients due to oral ingestion.
AMERICAN JOURNAL OF EMERGENCY MEDICINE
(2022)
Article
Food Science & Technology
Ana Ruiz de la Bastida, Angela Peiroten, Susana Langa, Juan Luis Arques, Jose Maria Landete
Summary: Isoflavones in soybeans can be transformed into equol and 5-hydroxy-equol by intestinal microbiota, with certain lactic acid bacteria strains being able to enhance their production. Among these, L. fermentum strains have shown particular effectiveness in producing these compounds.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Review
Food Science & Technology
Haritha Meruvu, Sebnem Tellioglu Harsa
Summary: This review comprehensively describes current research on lactic acid bacteria (LAB), including their sources, growth requisites, isolation and characterization strategies, and their applications in various sectors. The review also details various industrial patents related to the isolation and identification of novel LAB strains, methods and media for detection/screening, isolation, adaptation, culturing, preservation, and growth improvement, as well as the techniques and methodologies supporting LAB fermentation and the applications of the produced industrial metabolites.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Reza Mohammadi, Mehran Moradi, Hossein Tajik, Rahim Molaei
Summary: An antimicrobial membrane was developed using postbiotics from FreshQ and bacterial nanocellulose (BNC). The postbiotics exhibited antibacterial activity against various strains, and the wet form of the BNC showed higher antimicrobial activity compared to the lyophilized form. The fabricated material has potential applications in the food industry.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Food Science & Technology
Maulik Patel, Writdhama Prasad, Harshita Naithani, Basavaprabhu Haranahalli Nataraj, Sumit Arora, Pradip Behare
Summary: This study investigated the iron-binding ability of LAB producing EPS, with Lacticaseibacillus rhamnosus Kar1 strain showing significantly higher iron complexing ability. The findings suggest that LAB strains like L. rhamnosus Kar1 and its EPS can be further explored for developing iron-fortified whey-based products.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Tingting Xiao, Nagendra P. Shah
Summary: The study investigated the mechanism of GABA production using a co-culture of GABA-producing Lb. brevis 145 and Streptococcus thermophilus 1275. The co-culture showed enhanced sugar utilization, lactic acid production, improved GABA production, and a decrease in MSG level during fermentation. Correlation between GABA production and sugar utilization was weak, with the acidic environment created by lactic acid playing a crucial role in influencing GABA synthesis.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Engineering, Chemical
Liya Wang, Ryosuke Isobe, Yasunori Okano, Masahiro Kino-Oka, Hiroyuki Matsuda, Tomohiro Tokura
Summary: Numerical simulations were conducted in this study to evaluate the performance of a newly developed orbitally shaken bioreactor equipped with a vaulted bump at the bottom wall. The presence of this bump significantly improved mass transfer and cell suspension ratio without increasing shear stress, while efficiently reducing cell aggregation in the central part of the bottom wall. The Bayesian algorithm was employed to optimize three parameters of the reactor, leading to a more suitable cell culture environment for practical applications.
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
(2021)
Article
Engineering, Chemical
Liya Wang, Ryosuke Isobei, Yasunori Okano, Masahiro Kino-Oka
Summary: In this study, numerical simulation was used to compare the performance of two different shaking methodologies in an orbitally shaken bioreactor. Results showed that the PAR method outperformed the ODR method in terms of cell suspension, distribution, and shear stress. The parametric study of the PAR method further revealed an optimum culture condition for cell quality and energy consumption, providing new insights for bioreactor design and shaking techniques in suspension culture.
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
(2021)
Article
Computer Science, Interdisciplinary Applications
Yusuke Hayashi, Masahiro Kino-oka, Hirokazu Sugiyama
Summary: The study presents a hybrid-model-based design of fill-freeze-thaw processes for hiPS cells, optimizing quality and productivity indicators. Experimental results show that the accumulation of intracellular reactive oxygen species during filling and thawing serves as the quality indicator, while the duration of filling and thawing affects productivity. In the thawing process, vial diameter significantly impacts productivity.
COMPUTERS & CHEMICAL ENGINEERING
(2022)
Article
Cell & Tissue Engineering
Mee-Hae Kim, Naruchit Thanuthanakhun, Shun Fujimoto, Masahiro Kino-oka
Summary: Understanding the cell behavior-driven epigenetic memory in hiPSC cultures is crucial for achieving stability and robustness of cell differentiation. Changes in growth-related cell behavior can induce nucleoskeletal reorganization and modifications, allowing hiPSCs to alter their differentiation potential. This memory effect can last for several days and has a sustained impact on cell lineage specification during hiPSC differentiation.
STEM CELL RESEARCH
(2021)
Article
Engineering, Chemical
Keita Hirono, Isuru A. Udugama, Yusuke Hayashi, Masahiro Kino-oka, Hirokazu Sugiyama
Summary: This study proposes a five-step method that incorporates system dynamics and variabilities to determine the design space of mesenchymal stem cell (MSC) cultivation processes. The method uses mechanistic models and stochastic simulation to capture the dynamics of MSC cultivation and consider influences of variabilities at different levels. By simulating the timedependent changes in critical quality attributes (CQAs), a dynamic and probabilistic design space is determined, followed by a global sensitivity analysis. The method is applied to an MSC cultivation case study and successfully identifies maximum cell density as a dominant parameter of interest. Sensitivity analysis is further conducted to evaluate the impact of changing substrate material specification on the design space, resulting in a broader and viable design space.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2022)
Article
Cell & Tissue Engineering
Manabu Mizutani, Kentaro Nakajima, Masahiro Kino-oka
Summary: Recent developments in cell-based product manufacturing have focused on advancing products to clinical trials or commercialization while considering the cost-effectiveness of cell manufacturing. This study developed an estimation method for resource expenditure in cell-processing operations and found that mechanization can improve reproducibility and stability, reducing costs over the product's life cycle. Additionally, installing a robotic operating system in cell manufacturing can reduce labor costs.
REGENERATIVE THERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Adithya Nair, Ikki Horiguchi, Kazuhiro Fukumori, Masahiro Kino-oka
Summary: This study proposes quality indices for the filling process of human-induced pluripotent stem cell products and evaluates the instability of the process using an algorithm. The results show that different quality indices exhibit different types of fluctuations during the filling process, with the highest variation found in cell potential between batches.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Computer Science, Interdisciplinary Applications
Benedikt X. Scholz, Yusuke Hayashi, Isuru A. Udugama, Masahiro Kino-oka, Hirokazu Sugiyama
Summary: This study presents a multilayered model-based approach for scaling up slow and forced convection-based freezing of human induced pluripotent stem cells. The approach combines a hybrid single-cell freezing model with a computational fluid dynamics model, and takes into account the spatial and timedependent temperature profiles within the freezer unit in calculating the cell survival rate.
COMPUTERS & CHEMICAL ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Mee-Hae Kim, Naruchit Thanuthanakhun, Masahiro Kino-oka
Summary: This study demonstrates a novel method using botulinum hemagglutinin (HA) to synchronize behavior-driven mechanical memory in human induced pluripotent stem cell (hiPSC) cultures. The application of HA can lead to cytoskeleton rearrangement and mechanical memory synchronization, resulting in increased pluripotent cell homogeneity. Synchronized hiPSCs have improved differentiation capability and efficiency in hepatocyte differentiation.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Review
Cell & Tissue Engineering
Atsunori Tsuchiya, Shuji Terai, Ikki Horiguchi, Yasuhiro Homma, Atsuhiro Saito, Norimasa Nakamura, Yoji Sato, Takahiro Ochiya, Masahiro Kino-oka
Summary: Extracellular vesicles (EVs) have gained attention as a new therapeutic tool, but they are still in the research and development stage. The general public lacks awareness and understanding of safety and efficacy risks, laws and regulations, and global research and development trends related to EV use. The Japanese Society for Regenerative Medicine expresses concerns about solely practitioner-based treatments and advocates for evidence-based treatments. EVs and processed cells have similarities in manufacturing processes and safety hazards, but their mechanisms of action and quality control strategies need further investigation. In Japan, non-commercial clinical research and individual medical treatments involving EVs are currently outside the scope of existing laws.
REGENERATIVE THERAPY
(2022)
Review
Biotechnology & Applied Microbiology
Naruchit Thanuthanakhun, Mee-Hae Kim, Masahiro Kino-oka
Summary: This review discusses the role of cell behavioral and mechanical homeostasis in modulating PSC states and functions during culture processes. Promising ways to manipulate culture variability through regulating cell behaviors are outlined, with potential implementation for designing a culture strategy based on Waddington's epigenetic landscape concept.
BIOENGINEERING-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Takaki Matsumoto, Mee-Hae Kim, Masahiro Kino-oka
Summary: This study investigated the effects of ROCK inhibitor on the growth behaviors of hiPSCs in suspension culture, finding that long-term exposure to ROCK inhibitor increased cell division and enhanced the growth abilities of hiPSCs in suspension culture.
BIOENGINEERING-BASEL
(2022)
Article
Engineering, Biomedical
Mee-Hae Kim, Shao Ying Tan, Kenichi Yamahara, Masahiro Kino-Oka
Summary: Assessing the ability of cultured cells to degrade extracellular matrix (ECM) is crucial for evaluating the effectiveness of mesenchymal stem cell (MSC)-based therapies. However, current methods to assess this ability in vitro are lacking. In this study, a simple culture platform using high-density collagen surfaces was developed to study the ECM remodeling potential of cultured MSCs. This platform was used to evaluate the ECM remodeling potential of long-term ex vivo-expanded MSCs in vitro.
ACTA BIOMATERIALIA
(2023)
Article
Biochemical Research Methods
Mee-Hae Kim, Naruchit Thanuthanakhun, Masahiro Kino-oka
Summary: This study establishes a simple and efficient method for generating synchronous iPSC-derived pancreatic progenitors using botulinum hemagglutinin for cell synchronization. The method can produce high homogeneity and large quantities of pancreatic progenitor cells, and shows stable and robust differentiation across different cultures and runs.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Cell & Tissue Engineering
Suman C. Nath, Bradley Day, Lane Harper, Jeffrey Yee, Charlie Yu-Ming Hsu, Leila Larijani, Leili Rohani, Nicholas Duan, Michael S. Kallos, Derrick E. Rancourt
Summary: Research has shown that murine embryonic stem cells exhibit higher expression of pluripotency marker genes in stirred suspension bioreactors, a phenomenon known as mechanopluripotency. Fluid shear stress promotes the nuclear translocation of beta-catenin, leading to increased expression of c-Myc, an upstream regulator of pluripotency genes such as Oct4, Sox2, and Nanog. The mechanotransduction through adherens junction complex plays a crucial role in maintaining pluripotency of mESCs.
