Regulating unfolded protein response activator HAC1p for production of thermostable raw-starch hydrolyzing α-amylase in Pichia pastoris

Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris

(2015) Cheng Li et al.   BMC BIOTECHNOLOGY

Codon optimization, promoter and expression system selection that achieved high-level production of Yarrowia lipolytica lipase in Pichia pastoris

(2015) Wen-Jing Zhou et al.   ENZYME AND MICROBIAL TECHNOLOGY

Combined use of GAP and AOX1 promoters and optimization of culture conditions to enhance expression of Rhizomucor miehei lipase

(2015) Dong He et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Bioprocess exploration for thermostableα-amylase production of a deep-sea thermophileGeobacillussp. in high-temperature bioreactor

(2015) Tao Jiang et al.   PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY

Multiple copies of the target gene enhances plectasin secretion in Pichia pastoris X-33

(2015) Da Teng et al.   PROCESS BIOCHEMISTRY

Characterization of a thermostable raw-starch hydrolyzing α-amylase from deep-sea thermophile Geobacillus sp.

(2015) Tao Jiang et al.   PROTEIN EXPRESSION AND PURIFICATION

Engineering of Protein Folding and Secretion—Strategies to Overcome Bottlenecks for Efficient Production of Recombinant Proteins

(2014) Marizela Delic et al.   ANTIOXIDANTS & REDOX SIGNALING

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The Transcription Factor Titration Effect Dictates Level of Gene Expression

(2014) Robert C. Brewster et al.   CELL

Synergistic modular promoter and gene optimization to push cellulase secretion by Pichia pastoris beyond existing benchmarks

(2014) Andrea Mellitzer et al.   JOURNAL OF BIOTECHNOLOGY

High-temperature cultivation of recombinant Pichia pastorisincreases endoplasmic reticulum stress and decreases production of human interleukin-10

(2014) Yongjun Zhong et al.   Microbial Cell Factories

Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor production in controlled fed-batch cultures

(2014) Ana Vanz et al.   Microbial Cell Factories

Towards improved membrane protein production in Pichia pastoris: General and specific transcriptional response to membrane protein overexpression

(2014) Thomas Vogl et al.   New Biotechnology

Codon optimization, expression, purification, and functional characterization of recombinant human IL-25 in Pichia pastoris

(2013) Yushan Liu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Expression of the lasB gene encoding an organic solvent-stable elastase in Pichia pastoris and potential applications of the recombinant enzymes in peptide synthesis

(2013) Minghai Han et al.   BIOCHEMICAL ENGINEERING JOURNAL

Codon Optimization Significantly Improves the Expression Level of a Keratinase Gene in Pichia pastoris

(2013) Hong Hu et al.   PLoS One

Combined strategies for improving the heterologous expression of an alkaline lipase from Acinetobacter radioresistens CMC-1 in Pichia pastoris

(2013) Xiaolan Zhao et al.   PROCESS BIOCHEMISTRY

Synthetic Core Promoters for Pichia pastoris

(2013) Thomas Vogl et al.   ACS Synthetic Biology

Catabolite Repression of Aox in Pichia pastoris Is Dependent on Hexose Transporter PpHxt1 and Pexophagy

(2010) P. Zhang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Production of raw cassava starch-degrading enzyme by Penicillium and its use in conversion of raw cassava flour to ethanol

(2010) Hai-Juan Lin et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins

(2010) Mouna Guerfal et al.   Microbial Cell Factories

Novel strategy for yeast construction using δ-integration and cell fusion to efficiently produce ethanol from raw starch

(2009) Ryosuke Yamada et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Directed gene copy number amplification inPichia pastorisby vector integration into the ribosomal DNA locus

(2009) Hans Marx et al.   FEMS YEAST RESEARCH