Article
Biotechnology & Applied Microbiology
Fusang Ji, Lu Tang, Zhen Yang, Yuanyuan Li, Wenchang Wang, Ya Xu, Shuangmei Li, Xinguo Li
Summary: This study utilized RNA-seq to analyze transcriptomic changes in banana roots under salt stress, identifying a total of 1466 and 2089 differentially expressed genes. Functional annotation analysis revealed the involvement of these genes in various aspects of salt stress response such as stress signal transduction, ROS scavenging, osmoregulation, and transcription factors.
PLANT BIOTECHNOLOGY REPORTS
(2021)
Article
Plant Sciences
Jijun Li, Sidra Iqbal, Yuting Zhang, Yahui Chen, Zengdong Tan, Usman Ali, Liang Guo
Summary: The comparative transcriptome analysis in two rapeseed lines under flooding stress reveals that transcription regulation and antioxidant activity are common response processes in rapeseed flooding. These processes may contribute to rapeseed flooding resistance.
Article
Plant Sciences
Ali Raza, Wei Su, Muhammad Azhar Hussain, Sundas Saher Mehmood, Xuekun Zhang, Yong Cheng, Xiling Zou, Yan Lv
Summary: Rapeseed is an important oilseed crop and enhancing cold stress tolerance is crucial for agricultural investigation and crop improvement. This study identified stress-responsive genes, metabolites, and pathways in cold-tolerant and cold-sensitive rapeseed varieties. Functional analysis revealed the significance of starch and sucrose metabolism and phenylalanine metabolism in cold stress adaption. Six candidate genes were selected for controlling adaption to low temperature and showed significant differences in freezing tolerance.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Lidong Hao, Shubing Shi, Haibin Guo, Jinshan Zhang, Peng Li, Yanfei Feng
Summary: Silicon plays a vital role in plant growth, and a study using RNA-seq technology in wheat seedlings treated with silicon revealed that many genes responded to silicon treatment. Among the differentially expressed genes (DEGs), 6.25% were predicted transcription factors, with 14.67% of these belonging to the MYB TF family. The study also identified 411 R2R3-MYB TFs in the wheat genome, showing their importance in the silicon response mechanism and potential for breeding silicon-tolerant wheat.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Lakshmi Kasirajan, Rabisha Valiyaparambth, Keerthana Kamaraj, Sheelamary Sebastiar, Nam V. Hoang, Selvi Athiappan, Vasantha Srinivasavedantham, Karthigeyan Subramanian
Summary: This study utilized suppression subtractive hybridization and sequencing to identify salt-tolerance transcripts in wild sugarcane. The results provide insights into the molecular response of sugarcane under salt stress and can contribute to the discovery of novel salt-tolerance genes and transcription factors.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Plant Sciences
Yi Zhang, Ali Raza, He Huang, Wei Su, Dan Luo, Liu Zeng, Xiaoyu Ding, Yong Cheng, Zhaofeng Liu, Quanan Li, Yan Lv, Xiling Zou
Summary: In this study, 35 Lhcb genes (BnLhcbs) were identified in the rapeseed genome, which play essential roles in photosynthesis and stress response. The BnLhcb3.4 gene was significantly induced in the tolerant rapeseed cultivar and down-regulated in the sensitive cultivar. Overexpression of BnLhcb3.4 gene enhanced the freezing tolerance of transgenic Arabidopsis and altered the expression of ABA-responsive genes.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Agriculture, Dairy & Animal Science
Hao Ding, Yueyue Lin, Tao Zhang, Lan Chen, Genxi Zhang, Jinyu Wang, Kaizhou Xie, Guojun Dai
Summary: The study utilized RNA sequencing to explore gene expression in pigeon skeletal muscle development, identifying differentially expressed genes across different developmental stages. Weighted gene co-expression network analysis (WGCNA) revealed gene modules correlated with pigeon muscle growth and embryonic development, providing insights into the molecular mechanisms. A KEGG pathway analysis highlighted pathways involved in pigeon skeletal muscle growth and development, with selected genes validated by qRT-PCR. This research enhances understanding of gene expression and regulation in pigeon muscle development.
Article
Biochemistry & Molecular Biology
Javier Canales, Jose F. Verdejo, Daniel F. Calderini
Summary: This study investigates the transcriptome and physiological responses of rapeseed to post-flowering temperature increases, providing valuable insights into the molecular mechanisms underlying rapeseed tolerance to heat stress. Results showed that heat stress negatively affects seed yield and seed number, with genotype-specific responses. The study also reveals tissue-specific responses to heat stress and highlights the role of protein folding in maintaining silique development and seed quality under high-temperature conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Entomology
Di Luo, Qian Liu, Jinfeng Wang, Roman Jashenko, Rong Ji
Summary: This study investigated the molecular mechanisms and differences in high temperature tolerance between Calliptamus italicus and Gomphocerus sibiricus. The transcriptomic response of the two locust species displayed different patterns, with heat shock protein genes being highly expressed under high-temperature stress. The fluorescence results confirmed the accuracy of the transcriptome results.
ENVIRONMENTAL ENTOMOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ali Raza, Wei Su, Ang Gao, Sundas Saher Mehmood, Muhammad Azhar Hussain, Wenlong Nie, Yan Lv, Xiling Zou, Xuekun Zhang
Summary: In this study, the CAT gene family in the rapeseed genome was comprehensively analyzed, identifying 14 BnCAT genes classified into four groups with distinct gene structures, conserved motifs, and responsive elements. Expression analysis revealed that several BnCAT genes showed significant upregulation in different tissues and under various stress conditions, providing valuable insights for future investigations on the CAT family genes in rapeseed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Minjun Kim, Yoonji Chung, Prabuddha Manjula, Dongwon Seo, Sunghyun Cho, Eunjin Cho, Thisarani Kalhari Ediriweera, Myunghwan Yu, Sunju Nam, Jun Heon Lee
Summary: In this study, RNA sequencing was used to observe the time-dependent host responses of Eimeria-infected chickens, providing insights into the genes and biological functions associated with immunity to the parasite. The results revealed activation of endoplasmic reticulum stress and innate immune responses at the initial stage of infection, which gradually normalized over time. Additionally, the construction of gene co-expression networks identified key immunoregulation genes and pattern recognition receptors. These findings contribute to our understanding of the host-pathogen interaction and provide potential targets for improving coccidiosis control in chickens.
FRONTIERS IN GENETICS
(2022)
Article
Biochemistry & Molecular Biology
Byeong Hee Kang, Woon Ji Kim, Sreeparna Chowdhury, Chang Yeok Moon, Sehee Kang, Seong-Hoon Kim, Sung-Hwan Jo, Tae-Hwan Jun, Kyung Do Kim, Bo-Keun Ha
Summary: This study identified genes related to salt stress in cowpea by comparing germplasms with contrasting salt tolerance. The results provide useful information for the development of molecular markers in cowpea breeding programs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Wei Su, Ali Raza, Ang Gao, Ziqi Jia, Yi Zhang, Muhammad Azhar Hussain, Sundas Saher Mehmood, Yong Cheng, Yan Lv, Xiling Zou
Summary: The study identified 31 BnSOD genes in rapeseed, including 14 BnCSDs, 11 BnFSDs, and 6 BnMSDs. Phylogenetic analysis showed that the SOD genes from rapeseed and related plant species were clustered into three groups based on domain binding. The genes experienced segmental duplications and displayed well-maintained exon-intron structures and motifs within groups.
Article
Veterinary Sciences
Chunhai Li, Yan Yan, Cheng Pan, Michael Adjei, Khuram Shahzad, Peng Wang, Meilan Pan, Kerui Li, Ye Wang, Wangsheng Zhao
Summary: In this study, differentially expressed circRNAs in the epididymis of yak and cattleyak were identified, with a total of 1,298 circRNAs discovered and 137 of them showing differential expression. The functions of some circRNAs were elucidated and verified using qPCR. GO enrichment analysis revealed that DE circRNAs were mainly associated with metabolic process, development process, immune system process, reproductive process, reproduction, biological adhesion and growth. COG classification analysis showed that DE circRNAs derived genes were primarily involved in replication, recombination and repair. KEGG pathway analysis suggested that DE circRNAs were mainly involved in RNA degradation. Additionally, a circRNA binding miRNA, Bta-mir-103, related to sperm activity, was also screened.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Multidisciplinary Sciences
Dipayan Das, Subires Bhattacharyya, Monidipa Bhattacharyya, Puja Sashankar, Arindam Ghosh, Palash Mandal
Summary: This study investigates the molecular expression patterns and differentially expressed genes (DEGs) related to the extension of mulberry leaf shelf-life. The results provide insights into the molecular mechanisms involved in shelf-life extension, which could help address existing issues in the sericulture industry.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Chenglin Jiang, Ju Sun, Rui Li, Shijuan Yan, Wei Chen, Liang Guo, Guochen Qin, Pengcheng Wang, Cheng Luo, Wenjie Huang, Qinghua Zhang, Alisdair R. Fernie, David Jackson, Xiang Li, Jianbing Yan
Summary: This study investigated the molecular mechanism of haploid induction (HI) in maize and discovered a new gene controlling HI. The findings suggest that the balance of reactive oxygen species (ROS) plays a critical role in maintaining normal reproduction and provide a potential route to accelerate crop breeding.
Article
Plant Sciences
Ting Zhou, Pengjia Wu, Caipeng Yue, Jinyong Huang, Zhenhua Zhang, Yingpeng Hua
Summary: This study revealed differential responses of rapeseed to NO3- and NH4+ treatments under nitrogen-starved conditions through comparative transcriptomic analysis. Key genes involved in nitrogen uptake, including nitrogen transporters and transcription factors, were identified. The study also highlighted promising candidate genes for enhancing nitrogen use efficiency in rapeseed.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
Huirui Guan, Xin Liu, Yanping Fu, Xiaomin Han, Yanli Wang, Qing Li, Liang Guo, Luis A. J. Mur, Yahui Wei, Wei He
Summary: In this study, physiological and genetic analyses were conducted to show that A. oxytropis promotes growth and development in plants by regulating auxin, suggesting a possible role in benefiting its locoweed hosts independent of its toxin production.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biotechnology & Applied Microbiology
Xia Tian, Xiwen Yu, Zhijie Wang, Liang Guo, Jinxing Tu, Jinxiong Shen, Bin Yi, Tingdong Fu, Jing Wen, Chaozhi Ma, Cheng Dai
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Ying-peng Hua, Yi-fan Zhang, Tian-yu Zhang, Jun-fan Chen, Hai-li Song, Peng-jia Wu, Cai-peng Yue, Jin-yong Huang, Ying-na Feng, Ting Zhou
Summary: In this study, we found that both low Fe and NaCl significantly inhibited the growth of seminal roots in wheat seedlings. Sufficient Fe caused greater growth cessation of the seminal roots than low Fe under salt stress. Low Fe alleviated the salinity-induced growth cessation of seminal roots by responding to local low Fe signals in the roots.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Plant Sciences
Guofang Zhang, Jinzhi Zhou, Yan Peng, Zengdong Tan, Yuting Zhang, Hu Zhao, Dongxu Liu, Xiao Liu, Long Li, Liangqian Yu, Cheng Jin, Shuai Fang, Jiawei Shi, Zedong Geng, Shanjing Yang, Guoxing Chen, Kede Liu, Qingyong Yang, Hui Feng, Liang Guo, Wanneng Yang
Summary: Salt stress affects the survival and growth of crops, and understanding the genetic resources related to salt stress tolerance in Brassica napus is important. This study quantified the salt stress tolerance of B. napus using a high-throughput phenotyping platform and identified candidate genes associated with salt stress response through genome-wide association study and linkage analysis.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Plant Sciences
Lin-Lin Hu, Li-Wei Zheng, Xin-Lei Zhu, Sheng-Jie Ma, Kai-Yan Zhang, Ying-Peng Hua, Jin-Yong Huang
Summary: In this study, histone modification genes (HMs) in Brassicaceae species were systematically analyzed, and their roles in response to abiotic stress were identified. The findings provide important insights into the biological roles of Brassicaceae HMs and potential development of stress-tolerant oilseed plants.
Article
Biotechnology & Applied Microbiology
Dongxu Liu, Guanbo Yan, Shengbo Wang, Liangqian Yu, Wei Lin, Shaoping Lu, Liang Guo, Qing-Yong Yang, Cheng Dai
Summary: This study conducted a detailed analysis of the transcriptomes of seven hormones in Brassica napus seedlings. It was found that these hormones regulate different members of protein families rather than having common target genes. The study also examined the regulatory networks between these hormones and identified a novel interaction between gibberellin and cytokinin, as well as the associated transcription factors. The results of the study provide a valuable resource for future hormone studies.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Qiyue Zhang, Nikabou Batigma Boundjou, Lijun Jia, Xinliang Wang, Ling Zhou, Helga Peisker, Qing Li, Liang Guo, Peter Dormann, Dianqiu Lyu, Yonghong Zhou
Summary: A mitochondrion-specific CDS, called CDS6, was identified and found to be crucial for the biosynthesis of phosphoglycerolipids in mitochondria, which are critical for mitochondrial structure, triacylglycerol degradation, energy production, and seedling development.
Article
Plant Sciences
Yuzhou Yang, Que Kong, Wan Ting Tee, Yuqing Li, Pui Man Low, Barunava Patra, Liang Guo, Ling Yuan, Wei Ma
Summary: bZIP52 physically interacts with and represses the transcriptional activity of WRINKLED1 to negatively regulate seed oil accumulation in Arabidopsis. bZIP52 acts by inhibiting the transcriptional activity of AtWRI1 and thereby reducing the expression of fatty acid biosynthetic genes in Arabidopsis, resulting in a reduction in oil production.
Article
Plant Sciences
Yan Peng, Hongxiang Lou, Zengdong Tan, Zhewen Ouyang, Yuting Zhang, Shaoping Lu, Liang Guo, Bao Yang
Summary: This study investigated the changes in lipidome and metabolome in rapeseed under nitrogen starvation. It was found that nitrogen deficiency affected plant growth and amino acid levels, and also altered membrane lipid metabolism and carbon metabolism. This research provides valuable information for further understanding the metabolic response of rapeseed to nitrogen deficiency.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Agronomy
Guihong Liang, Yingpeng Hua, Haifei Chen, Jinsong Luo, Hongkun Xiang, Haixing Song, Zhenhua Zhang
Summary: Nitrogen is crucial for plant growth, and its coordinated distribution is important for seed development and crop yield. Comparing high and low nitrogen use efficiency in rapeseed, it was found that leaf senescence promoted amino acid allocation in the high nitrogen use efficiency genotype. Efficient source to sink remobilization of amino acids led to higher branch and pod numbers in the high nitrogen use efficiency genotype under nitrogen deficiency. A 15N tracer experiment confirmed that more amino acids were allocated to seeds in the high nitrogen use efficiency genotype, mostly due to genetic variations in organic nitrogen transport and metabolism. These findings indicate that strong amino acid remobilization in rapeseed contributes to high yield, nitrogen use efficiency, and harvest index.
Article
Biotechnology & Applied Microbiology
Zengdong Tan, Yan Peng, Yao Xiong, Feng Xiong, Yuting Zhang, Ning Guo, Zhuo Tu, Zhanxiang Zong, Xiaokun Wu, Jiang Ye, Chunjiao Xia, Tao Zhu, Yinmeng Liu, Hongxiang Lou, Dongxu Liu, Shaoping Lu, Xuan Yao, Kede Liu, Rod J. Snowdon, Agnieszka A. Golicz, Weibo Xie, Liang Guo, Hu Zhao
Summary: This study comprehensively characterizes the gene regulatory features in Brassica napus seeds and reveals the gene networks regulating the seed oil content.
Article
Biochemistry & Molecular Biology
Tianyu Zhang, Ting Zhou, Yifan Zhang, Junfan Chen, Haili Song, Pengjia Wu, Caipeng Yue, Jinyong Huang, Zhenhua Zhang, Yingpeng Hua
Summary: This study identified the core ATG8 homologs and characterized their roles in stress resistance in rapeseed. The expression of BnaA8.ATG8F was found to improve salt stress tolerance by regulating root Na+ retention, and also increase sensitivity to nitrogen limitation by affecting nitrogen uptake and remobilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Yuhong Zhou, Huiyang Yu, Yaping Tang, Rong Chen, Jinying Luo, Chunmei Shi, Shan Tang, Xin Li, Xinyan Shen, Rongfeng Chen, Yuyang Zhang, Yongen Lu, Zhibiao Ye, Liang Guo, Bo Ouyang
Summary: Plant mitochondrial fatty acid synthesis (mtFAS) plays an important role in tomato development. A mutant tomato was identified that displayed pleiotropic phenotypes and affected tomato morphogenesis by influencing photorespiration, energy supply, and reactive oxygen species homeostasis. The mtFAS pathway may modulate tomato development by influencing mitochondrial membrane lipid composition and other lipid metabolic pathways.