Review
Geriatrics & Gerontology
Yuying Guan, Jia Liu, Yakun Gu, Xunming Ji
Summary: Cerebrovascular microcirculation is crucial for brain function, and angiogenesis plays a significant role in remodeling the microcirculation. Hypoxia is an important regulator of angiogenesis, but it also negatively affects cerebral vascular tissue. Establishing a model that promotes cerebral microvasculogenesis without causing injury is essential.
Article
Cell Biology
Sandra Dienemann, Vanessa Schmidt, Tabea Fleischhammer, Julia H. H. Mueller, Antonina Lavrentieva
Summary: In vitro cultivation conditions are important for understanding cell physiology and responses to external stimuli. Oxygen concentrations are crucial in influencing cell behaviors in both 2D and 3D cultures. This study genetically modified two types of human endothelial cells and monitored their responses to different oxygen concentrations. The findings demonstrated different hypoxic signatures for these cells in both 2D and 3D culture systems.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Oncology
Vaishali Chaurasiya, Dan Duc Pham, Jukka Harju, Anne Juuti, Anne Penttila, Sharath Kumar Goud Emmagouni, Van Dien Nguyen, Birong Zhang, Sanni Perttunen, Salla Keskitalo, You Zhou, Kirsi H. Pietilainen, P. A. Nidhina Haridas, Vesa M. Olkkonen
Summary: Communication between adipocytes and endothelial cells plays an important role in the metabolic function of white adipose tissue. This study developed a method to isolate adipose microvascular endothelial cells (AMvEC) from visceral adipose tissue (VAT) and established a co-culture system to study their communication. Various experiments validated the identity and functionality of the isolated cells. Additionally, the co-culture system can be used for drug screening.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Engineering, Biomedical
Yue Zhang, Meng Li, Yunchuan Wang, Fei Han, Kuo Shen, Liang Luo, Yan Li, Yanhui Jia, Jian Zhang, Weixia Cai, Kejia Wang, Ming Zhao, Jing Wang, Xiaowen Gao, Chenyang Tian, Baolin Guo, Dahai Hu
Summary: Chronic diabetic wounds are a globally recognized clinical challenge, and this study found that using exosome-based bioactive dressings as an alternative method for diabetic wound healing has great potential. However, research on multifunctional hydrogels loaded with drugs and bioactive substances that synergistically promote wound repair is lacking, and the mechanism of their combinatorial effect remains unclear.
BIOACTIVE MATERIALS
(2023)
Review
Dermatology
Daniel Bormann, Alfred Gugerell, Hendrik Jan Ankersmit, Michael Mildner
Summary: Cell secretomes play an important role in wound healing by promoting regeneration. This study reviews the mechanisms of action of cell secretomes in wound healing, important preconditioning strategies to enhance therapeutic efficacy, and clinical trials on secretome-based wound healing.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Neurosciences
Wen Zeng, Qiaoling Lei, Jiao Ma, Shuqiang Gao, Rong Ju
Summary: This study investigated the biological effects of EPC-derived MVs on BMECs, showing that EPC-MVs can enhance BMEC viability, proliferation, migration, and tube formation. These results suggest the proangiogenic effect of EPC-derived MVs on BMECs, which holds potential applications in cell-free therapy for brain injury.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Philipp Kuhn, Monika Bubel, Martina Jennewein, Silke Guthoerl, Tim Pohlemann, Martin Oberringer
Summary: This study examines the interaction of different types of stromal cells with endothelial cells during soft tissue healing. The results show that the functions of fibroblasts, pericytes, and adipose-derived stem cells are density-dependent when they interact with microvascular endothelial cells in cocultures. Juvenile fibroblasts mainly support angiogenesis through paracrine regulation, while this function may be compromised in adult fibroblasts. In contrast, pericytes and adipose-derived stem cells have more versatile roles aiming at angiogenesis, vessel stabilization, and tissue contraction.
CELL BIOCHEMISTRY AND FUNCTION
(2022)
Article
Cardiac & Cardiovascular Systems
Santeri Tarvainen, Galina Wirth, Greta Juusola, Olli Hautero, Kari Kalliokoski, Tanja Sjoros, Veikko Nikulainen, Jouni Taavitsainen, Jarkko Hytonen, Crister Frimodig, Krista Happonen, Tuomas Selander, Tomi Laitinen, Harri H. Hakovirta, Juhani Knuuti, Nihay Laham-Karam, Juha Hartikainen, Kimmo Makinen, Seppo Yla-Herttuala, Petra Korpisalo
Summary: This explorative study aimed to investigate the role of microvasculature in critical limb-threatening ischaemia (CLTI) pathology. High-resolution imaging of CLTI patients and muscle samples from amputated limbs were used to describe microvascular pathology. The findings suggest that CLTI muscles have altered blood flow dynamics and tissue damage due to hypoxia-driven capillary enlargement.
EUROPEAN HEART JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Ekta Manocha, Alessandra Consonni, Fulvio Baggi, Emilio Ciusani, Valentina Cocce, Francesca Paino, Carlo Tremolada, Arnaldo Caruso, Giulio Alessandri
Summary: Pericytes, as a type of mesenchymal stromal cells, have a crucial role in tissue regeneration and vascular homeostasis. This study found that CD146(+) pericytes from adipose tissue may have a higher efficacy in promoting angiogenesis and vascular stabilization compared to CD146(-) pericytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Walker D. Short, Emily Steen, Aditya Kaul, Xinyi Wang, Oluyinka O. Olutoye, Hima V. Vangapandu, Natalie Templeman, Alexander J. Blum, Chad M. Moles, Daria A. Narmoneva, Timothy M. Crombleholme, Manish J. Butte, Paul L. Bollyky, Sundeep G. Keswani, Swathi Balaji
Summary: This study found that overexpression of IL-10 in dermal wounds recruits EPCs, increases vascular structures, and accelerates re-epithelialization.
Article
Engineering, Environmental
Han Peng, Huichen Li, Xi Zhang, Jiezhang Tang, Yongping Liang, Lipeng Qiao, Yun Zhu, Mengmeng Hou, Siming Wei, Zhaoxiang Zhang, Chaohua Liu, Xinmao Li, Baoyan Liang, Baoqiang Song, Baolin Guo, Jie Zhang
Summary: In this study, a multi-functional hydrogel with 3D-exosome-sustained release features was developed to improve diabetic wound healing. It exhibited tissue-adhesive, self-healing, antibacterial, anti-inflammatory, and antioxidant properties. The hydrogel promoted angiogenesis and collagen deposition, accelerated the recovery of diabetic wounds, and achieved rapid hemostasis.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Biomedical
Que Bai, Caiyun Zheng, Na Sun, Wenting Chen, Qian Gao, Jinxi Liu, Fangfang Hu, Tong Zhou, Yanni Zhang, Tingli Lu
Summary: This study presents an oxygen-releasing hydrogel that promotes cell survival, migration, and tube formation under hypoxic conditions, and facilitates tissue repair by reducing inflammation, increasing angiogenesis, and collagen deposition.
ACTA BIOMATERIALIA
(2022)
Article
Ophthalmology
Xiaoling Shi, Nuo Dong, Qi Qiu, Shanhua Li, Jiaxing Zhang
Summary: The study demonstrates that salidroside (SAL) protects against hypoxia-induced human retinal microvascular endothelial cells (HRMECs) injury by regulating the miR-138/ROBO4 axis.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Review
Surgery
Rald V. M. Groven, Johan van Koll, Martijn Poeze, Taco J. Blokhuis, Martijn van Griensven
Summary: Fracture healing is a complex process involving cellular communication and different cell types. miRNAs play a crucial role in cellular communication, influencing gene expression significantly.
FRONTIERS IN SURGERY
(2021)
Article
Biochemistry & Molecular Biology
Elle Koivunotko, Jasmi Snirvi, Arto Merivaara, Riina Harjumaki, Swarna Rautiainen, Minna Kelloniemi, Kirsi Kuismanen, Susanna Miettinen, Marjo Yliperttula, Raili Koivuniemi
Summary: Adipose-derived mesenchymal stromal cells (ASCs) hold great potential for cellular therapies and can differentiate into endothelial cells (ECs) and other angiogenic cell types. Nanofibrillated cellulose (NFC) hydrogel is a suitable matrix for 3D cell culture of ASCs and can stimulate angiogenesis. This study recommends the use of NFC hydrogel as an animal-free biomaterial scaffold for therapeutic angiogenesis and other cell therapy purposes.