Article
Food Science & Technology
Jing-Nan Meng, Ying-Ju Liu, Xing Shen, Juan Wang, Ze-Ke Xu, Yu Ding, Ross C. Beier, Lin Luo, Hong-Tao Lei, Zhen-Lin Xu
Summary: Investigating cereulide contamination requires detection of emetic B. cereus, cereulide, and/or its biomarkers. The primary method used for detection of emetic B. cereus is polymerase chain reaction, while whole genome sequencing is expected to become an alternative method in the future. PCR remains the gold standard for emetic B. cereus determination and liquid chromatography coupled with mass spectrometry is widely accepted for cereulide quantification, while emerging techniques such as fluorescence and immunoassay have also been applied to detect cereulide and its biomarkers. It is also necessary to develop rapid screening methods to enhance the detection of cereulide in large numbers of samples.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2022)
Article
Food Science & Technology
Zhaoying Bai, Meng Wang, Menghui Xie, Siyuan Liu, Yingying Liu, Ziquan Wang, Guoping Zhou, Zhiwei Sui
Summary: This study established a rapid and specific method for quantifying viable B. cereus using fluorescence-labeled antibodies and viability indicators in conjunction with flow cytometry. The method demonstrated notable specificity and robust anti-interference ability. The developed method enables the rapid and specific quantification of viable B. cereus, offering a novel approach to the detection of specific foodborne pathogens.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Ayaka Okada, Mizuki Tsuchida, Kazuha Aoyagi, Ayaka Yoshino, Matiur Rahman, Yasuo Inoshima
Summary: Globally, Campylobacter spp. are important pathogens causing food-borne gastroenteritis. Conventional culture methods cannot detect viable but nonculturable (VBNC) bacteria. In this study, a PMA-qPCR assay was used to detect viable Campylobacter spp. in chicken meat, and the detection rates of PMA-qPCR and the culture method were compared. The results suggest that both methods should be used in parallel to increase the detection rate of Campylobacter spp.
Article
Food Science & Technology
Haibo Zhou, Xinmei Liu, Zhaoxin Lu, Antuo Hu, Wenjie Ma, Changzheng Shi, Xiaomei Bie, Yiyu Cheng, Haijing Wu, Jun Yang
Summary: A new method called PMA-duplex ddPCR was developed for the quantitative determination of viable Vibrio parahaemolyticus in aquatic products, with lower limit of detection and high accuracy. This method allows for the detection of V. parahaemolyticus without pre-enrichment.
Article
Microbiology
Ayaka Okada, Mizuki Tsuchida, Md. Matiur Rahman, Yasuo Inoshima
Summary: This study presents an optimized PMA treatment method for the detection of VBNC Campylobacter spp. in chicken meat using the PMA-qPCR approach, effectively solving the issue of undetectable bacteria through conventional culture methods.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Chih-Hung Wang, Jia-Ru Chang, Shang-Cheng Hung, Horng-Yunn Dou, Gwo-Bin Lee
Summary: Tuberculosis is a serious global public health issue and early diagnosis is challenging due to the slow growth of Mycobacterium tuberculosis. This study presents an integrated microfluidic system that can automatically detect live M. tuberculosis and distinguish dead bacteria. The system offers automation, high speed, low cost, low sample/reagent consumption, and reduced infection risk for medical staff.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemistry & Molecular Biology
Kyung-Ho Kim, Gyoungsik Kang, Won-Sik Woo, Min-Young Sohn, Ha-Jeong Son, Chan-Il Park
Summary: Red sea bream iridovirus (RSIV) is a significant aquatic virus causing high mortality in marine fish. A viability qPCR assay based on PMAxx was developed, which effectively differentiated between infectious and inactive viruses and detected infectious RSIV more efficiently than conventional methods. This method can prevent overestimation of red sea bream iridoviral disease caused by RSIV.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Lei Wang, Huansen Yang, Kun Wang, Haitao Yang, Mengdi Zhao, Yuping Shang, Fang Wang, Jingquan Dong, Weiguo Zhao, Li Li, Wei Liang, Yan Wang
Summary: A specific, rapid, and sensitive detection method for Bacillus cereus in dairy products has been developed, which can detect the bacteria within 20 minutes at 37°C with no cross-reactivity with other foodborne pathogens. This method is effective for distinguishing emetic from non-emetic strains in dairy products.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Food Science & Technology
Shuo Yang, Yating Wang, Yangtai Liu, Kai Jia, Zhen Zhang, Qingli Dong
Summary: Cereulide, produced by Bacillus cereus, is a stable emetic toxin with high toxicity. There is a need for a better understanding of the impact of B. cereus and cereulide to prevent contamination and protect public health. Summarized information highlighting precautions at the public level is lacking, hence the aim of this review is to propose such precautions.
Article
Biochemistry & Molecular Biology
Eva Maria Kalbhenn, Markus Kranzler, Agnieszka Gacek-Matthews, Gregor Grass, Timo D. Stark, Elrike Frenzel, Monika Ehling-Schulz
Summary: Bacillus cereus causes emetic type foodborne disease through production of the small peptide toxin cereulide. The CesNRPS multienzyme machinery, encoding Ces nonribosomal peptide synthetase, is located on a plasmid designated pCER270. Chromosomal transcriptional factors CodY and AbrB control ces transcription. In addition, a plasmid-encoded transcription factor PagRBc, homologous to PagR in Bacillus anthracis, also plays an important role in the regulation of cereulide synthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Qin Xiong, Ju Liu, Guoyang Xie, Dan Mu, Xiaoyan Feng, Hengyi Xu
Summary: A new detection method for emetic Bacillus cereus was established in this study, showing sensitive and specific detection results in laboratory conditions.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Xiaoyan Feng, Donggen Zhou, Dan Mu, Hengyi Xu
Summary: Escherichia coli O157:H7 is a harmful bacterium that produces toxins causing thrombotic thrombocytopenic purpura and haemolytic uraemic syndrome. Real-time recombinase-aided amplification (rRAA) can detect pathogens within 20 min but cannot differentiate between dead and viable bacteria, leading to false-positive results. By integrating thiazole orange monoazide (TOMA) with rRAA, a sensitive assay was developed, allowing for rapid identification of viable E. coli O157:H7 in milk with a detection limit of less than 10 cfu mL-1, and resistance to interference from non-target bacteria and high concentrations of dead bacteria.
INTERNATIONAL DAIRY JOURNAL
(2023)
Article
Food Science & Technology
Eva Maria Kalbhenn, Tobias Bauer, Timo D. Stark, Mandy Knuepfer, Gregor Grass, Monika Ehling-Schulz
Summary: A new method for the detection and isolation of cereulide toxin using commonly available laboratory equipment was developed. Reproducible detection and relative quantification of cereulide was achieved through reversed phase chromatography, with specificity validated using a panel of strains.
Article
Horticulture
Eliezer Louzada, Omar Vazquez, Sandy Chavez, Mamoudou Setamou, Madhurababu Kunta
Summary: This research found the most reliable method for detecting only live CLas cells in citrus by testing primers amplifying various amplicon sizes.
Article
Biochemistry & Molecular Biology
Yao Liu, Caijiao Wei, Hui Wan, Sarengaowa Sarengaowa, Xiaoping Liang, Tao Jiang, Yuhe Dong, Xihong Zhao, Tian Zhong
Summary: A rapid and practical technique (PMA-mPCR) was developed for the simultaneous detection of viable E. coli O157:H7, S. aureus, and Salmonella in pure culture and in a food matrix. The use of propidium monoazide (PMA) at an optimum concentration of 5.0 μg/mL successfully eliminated false positive results. The PMA-mPCR assay showed a detection limit of 10(4) CFU/mL for both pure culture and food matrix samples.
Article
Agriculture, Dairy & Animal Science
Baoqing Zhou, Taobo Liang, Zhongxu Zhan, Rui Liu, Fan Li, Hengyi Xu
JOURNAL OF DAIRY SCIENCE
(2017)
Article
Agriculture, Dairy & Animal Science
Taobo Liang, Ping Zhou, Baoqing Zhou, Qian Xu, Ziqiang Zhou, Xin Wu, Zoraida P. Aguilar, Hengyi Xu
JOURNAL OF DAIRY SCIENCE
(2019)
Article
Biotechnology & Applied Microbiology
Guoyang Xie, Liehai Hu, Rui Liu, Min Huang, Taobo Liang, Zoraida P. Aguilar, Hengyi Xu
BIOCHEMICAL ENGINEERING JOURNAL
(2019)
Article
Agriculture, Dairy & Animal Science
Ju Liu, Zhongxu Zhan, Taobo Liang, Guoyang Xie, Zoraida P. Aguilar, Hengyi Xu
JOURNAL OF DAIRY SCIENCE
(2020)
Article
Food Science & Technology
Taobo Liang, Xin Wu, Bolu Chen, Ju Liu, Zoraida P. Aguilar, Hengyi Xu
LWT-FOOD SCIENCE AND TECHNOLOGY
(2020)
Article
Microbiology
Guoyang Xie, Shuang Yu, Wen Li, Dan Mu, Zoraida P. Aguilar, Hengyi Xu
JOURNAL OF MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Jingjing Zhang, Feng Qu, Taobo Liang, Sidi Lv, Wen Li, Qixiu You, Bolu Chen, Hengyi Xu
Summary: Synthesized Ag NPs coated with ε-polylysine and poly-ethyleneimine showed strong anti-P. aeruginosa capacity at low concentrations, inhibiting drug-resistant clinical strains and biofilm formation. The antibacterial mechanism was found to be primarily through induction of oxidative stress by increasing levels of ROS, which was neutralized by the ROS scavenger N-acetyl-L-cysteine.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Food Science & Technology
Guoyang Xie, Donggen Zhou, Guojing Zhao, Xiaoyan Feng, Zoraida P. Aguilar, Hengyi Xu
Summary: The study proposed a method for rapid and real-time detection of viable S. aureus in milk, combining PMAxx dye and RAA technology. It can accurately detect S. aureus in a short time, which is of great significance for effective monitoring of food safety.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Ju Liu, Guoyang Xie, Qin Xiong, Taobo Liang, Hengyi Xu
Summary: A sensitive and specific assay was developed for detecting Cronobacter spp. in powdered infant formula samples, showing the potential for rapid and efficient detection. The method could detect bacteria at low concentrations after enrichment with high recovery rates in both pure culture and spiked samples.
Article
Food Science & Technology
Taobo Liang, Hui Long, Zhongxu Zhan, Yingfei Zhu, Peilin Kuang, Ni Mo, Yuping Wang, Shenghui Cui, Xin Wu
Summary: This study describes a highly efficient, sensitive, specific, and simple multiplex real-time quantitative PCR (mRT-qPCR) method for the simultaneous detection of viable Salmonella spp., E. coli, and S. aureus. The method uses propidium monoazide (PMA) treatment to exclude dead bacteria, enabling the detection of only viable bacteria. The PMA-mRT-qPCR technique showed reliable distinction between dead and viable bacteria.
FOOD SCIENCE & NUTRITION
(2022)
Article
Nanoscience & Nanotechnology
Xiangyu Meng, Guotai Yang, Fulai Li, Taobo Liang, Weihua Lai, Hengyi Xu
ACS APPLIED MATERIALS & INTERFACES
(2017)
