Journal
MICROCHIMICA ACTA
Volume 186, Issue 11, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-019-3804-z
Keywords
Photoelectrochemistry; TATA-binding protein; Photoresponse; Nanoflowers; Electrode; Aptamer
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Funding
- National Natural Science Foundation of China [21775080, 21705086]
- Key Research and Development Project of Shandong Province, China [2017GSF221004]
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A photocathode is described for the determination of microRNA-21 by using CuInS2 as an active photocathode material. Exonuclease III assisted target recycling amplification was employed to enhance the detection sensitivity. The TATA-binding protein (TBP) was applied to enhance steric hindrance which decreases the photoelectrochemical intensity. This strategy is designed by combining the anti-interference photocathode material, enzyme assisted target recycling amplification and TBP induced signal off, showing remarkable amplification efficiency. Under the optimized conditions, the detection limit for microRNA-21 is as low as 0.47 fM, and a linear range was got from 1.0 x 10(-15) M to 1.0 x 10(-6) M.
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