Journal
BIOPHYSICAL JOURNAL
Volume 110, Issue 11, Pages 2451-2462Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2016.04.041
Keywords
-
Categories
Funding
- US National Science Foundation [CHE-1309731, NSF CHE-1307404]
- National Science Foundation Graduate Research Fellowship [NSF DGE-1144245]
- Springborn Fellowship
- Croucher Foundation Scholarship
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1309731] Funding Source: National Science Foundation
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1307404] Funding Source: National Science Foundation
Ask authors/readers for more resources
In this study, we examine the mechanism of flip-flop diffusion of proton carriers across the lipid layer of a hybrid bilayer membrane (HBM). The HBM consists of a lipid monolayer appended on top of a self-assembled monolayer containing a Cu-based O-2 reduction catalyst on a Au electrode. The flip-flop diffusion rates of the proton carriers dictate the kinetics of O2 reduction by the electrocatalyst. By varying both the tail lengths of the proton carriers and the lipids, we find the combinations of lengths that maximize the flip-flop diffusion rate. These experimental results combined with biophysical modeling studies allow us to propose a detailed mechanism for transmembrane flip-flop diffusion in HBM systems, which involves the bending of the alkyl tail of the proton carrier as the rate-determining step. Additional studies with an unbendable proton carrier further validate these mechanistic findings.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available