Article
Chemistry, Multidisciplinary
A. Dadashzadeh, S. Moghassemi, C. A. Amorim
Summary: This study demonstrated that PEGylated fibrin hydrogels enhanced scaffold stability, supported cell viability and proliferation. PEG:Fib 5:1 T50 showed a significantly higher cell density dynamic and a lower expression of caspase-3. In contrast, PEG:Fib 10:1 T50 hydrogels had uniform cell distribution and a higher rate of Ki67-positive cells.
MATERIALS TODAY CHEMISTRY
(2021)
Article
Engineering, Biomedical
Maximilian G. Burger, Andrea Grosso, Priscilla S. Briquez, Gordian M. E. Born, Alexander Lunger, Flavio Schrenk, Atanas Todorov, Veronica Sacchi, Jeffrey A. Hubbell, Dirk J. Schaefer, Andrea Banfi, Nunzia Di Maggio
Summary: In regenerative medicine, rapid vascularization of clinical-size bone grafts is a challenge that has not been solved. This study investigates the distribution of VEGF protein on matrices to achieve efficient coupling of angiogenesis and bone formation, providing a clinically applicable strategy for engineering vascularized bone.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Marie-Claire Regenberg, Mathias Wilhelmi, Andres Hilfiker, Axel Haverich, Thomas Aper
Summary: This study conducted a structural analysis of compacted fibrin tubes and found a strong correlation between fibrin's structure and its biomechanical strength. It also demonstrated that fibrin compaction can be enhanced through a dehydration process, leading to a significant increase in biomechanical strength. This method, combined with an adequate imaging technique, has potential as a powerful tool for quality assurance in the development of fibrin-based vascular grafts.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2023)
Article
Engineering, Biomedical
Andres Montero, Sonia Acosta, Rebeca Hernandez, Carlos Elvira, Jose Luis Jorcano, Diego Velasco
Summary: The study showed that temperature had minimal impact on the contraction rate of plasma-derived fibrin hydrogels, while the presence of plasma proteins significantly affected the contraction. Additionally, the addition of fibroblasts and keratinocytes also influenced the height of the hydrogels.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Review
Engineering, Biomedical
Derek W. Nelson, Ryan J. Gilbert
Summary: ECM-mimetic hydrogels are promising regenerative scaffolds for injured nervous system, providing 3D bioactive interfaces to modulate cellular response and support tissue remodeling. Future advancements in their design and use may lead to viable treatments for patients with neural injury.
ADVANCED HEALTHCARE MATERIALS
(2021)
Review
Engineering, Biomedical
Paula Pleguezuelos-Beltran, Patricia Galvez-Martin, Daniel Nieto-Garcia, Juan Antonio Marchal, Elena Lopez-Ruiz
Summary: Skin sprays, an innovative technique for treating acute and chronic wounds, offer great potential for delivering cells and hydrogels. Compared to conventional treatments, they have advantages such as ease of application, the ability to treat large wound areas, and uniform distribution of materials. This article reviews the latest advances in skin spray technology, including investigational and commercially available products, and discusses regulatory pathways and clinical trials for different skin diseases.
BIOACTIVE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Elisabetta Rosa, Carlo Diaferia, Eliana Gianolio, Teresa Sibillano, Enrico Gallo, Giovanni Smaldone, Mariano Stornaiuolo, Cinzia Giannini, Giancarlo Morelli, Antonella Accardo
Summary: In recent years, peptide-based hydrogels have been increasingly used in biomedical and pharmaceutical applications. This study demonstrates the synthesis and gelation properties of cationic peptides combined with hydrogelator FmocFF, resulting in the formation of novel self-sorted hydrogels with different mechanical properties and water mobility. In vitro studies show that most of the tested mixed hydrogels have no significant impact on cell viability, and one of the hydrogels shows potential as a synthetic extracellular matrix.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Chemistry, Medicinal
Olimpia Ortiz-Arrabal, Ainhoa Irastorza-Lorenzo, Fernando Campos, Miguel Angel Martin-Piedra, Victor Carriel, Ingrid Garzon, Paula Avila-Fernandez, Maria Jose de Frutos, Emilio Esteban, Javier Fernandez, Agustin Janer, Antonio Campos, Jesus Chato-Astrain, Miguel Alaminos
Summary: Marine-origin polysaccharides, especially agarose, have been extensively studied as scaffolds for tissue engineering. We have developed new fibrin-agarose (FA) biomaterials using different types and concentrations of agaroses, which showed high biocompatibility and varied biomechanical properties. In vivo experiments demonstrated the biocompatibility of FA tissues and histological analysis indicated a pro-regenerative process with M2-type CD206-positive macrophages. These findings confirm the clinical potential of FA biomaterials for tissue engineering applications, allowing for the selection of specific agarose types and concentrations to meet desired biomechanical properties and in vivo reabsorption times.
Article
Engineering, Biomedical
Hyeonji Kim, Je-Hwan Jang, Wonil Han, Hyun-Jeong Hwang, Jinah Jang, Joon Young Kim, Dong-Woo Cho
Summary: Sticky tissue sealants based on gelatinized cornea-derived extracellular matrix (GelCodE) show promise for tissue restoration and clinical application due to their ability to induce de novo tissue regeneration and form covalent bonds with adjacent tissues. This study demonstrates the ease of in vivo application, biocompatibility, biointegration, and exceptional de novo tissue formation of GelCodE sealant. Additionally, the newly regenerated tissues exhibit normal tissue-like matrices with minimal scar formation, presenting a promising strategy for scar-free tissue recovery in clinical settings.
Article
Engineering, Biomedical
Kaizheng Liu, Maury Wiendels, Hongbo Yuan, Changshun Ruan, Paul H. J. Kouwer
Summary: This article emphasizes the importance of three-dimensional hydrogel models in understanding and predicting cell behavior, highlighting the reciprocal interactions between cells and the extracellular matrix in regulating tissue functions. The structure, uses, advantages, and limitations of commonly used biopolymer networks, such as collagen, fibrin gels, and polyisocyanide gel, are discussed to provide guidance for tissue engineers and biophysicists in designing and utilizing these materials for 3D cell culture purposes.
BIOACTIVE MATERIALS
(2022)
Article
Materials Science, Biomaterials
Swatilekha Hazra, Souradeep Dey, Biman B. Mandal, Charanya Ramachandran
Summary: Silk films have potential as substrates for culturing human corneal endothelial cells. Despite their higher tensile strength compared to native basement membrane, the expression of extracellular matrix proteins and integrins on silk films is comparable to native tissue, confirming their suitability for engineering corneal endothelial cells for transplantation.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Engineering, Biomedical
Pujhitha Ramesh, Nicholas Moskwa, Zachary Hanchon, Adam Koplas, Deirdre A. Nelson, Kristen L. Mills, James Castracane, Melinda Larsen, Susan T. Sharfstein, Yubing Xie
Summary: In this study, cryoelectrospinning was used to fabricate scaffolds that mimic the structure and function of soft-tissue ECM. The researchers found that these scaffolds supported cell survival and exhibited potential for use in regenerative medicine applications.
Review
Biochemistry & Molecular Biology
Nurul H. Sulimai, Jason Brown, David Lominadze
Summary: This review discusses the role of fibrinogen and its derivatives in inflammatory diseases and their effects on immunomodulation.
Article
Biochemistry & Molecular Biology
Resmi Rajalekshmi, Anusree Kaladevi Shaji, Roy Joseph, Anugya Bhatt
Summary: The development of a tissue-engineered construct for hepatic regeneration is challenging due to the lack of an optimum environment for hepatocyte growth. Injectable alginate dialdehyde-gelatin hydrogel incorporating fibrin was explored as a matrix for liver tissue engineering, showing promising support for the growth and function of HepG2 cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Sebastian Blatt, Daniel G. E. Thiem, Andreas Pabst, Bilal Al-Nawas, Peer W. Kaemmerer
Summary: The combination of bone substitute materials (BSMs) with platelet-rich fibrin (PRF) showed significant positive pro-angiogenic effects, particularly with alloplastic and xenogeneic materials, leading to increased vessel formation in vivo. This demonstrates the enhanced angiogenetic potential of BSMs when combined with PRF, suggesting potential for bony regeneration in clinical applications.
Article
Pharmacology & Pharmacy
Rana B. Bakhaidar, Khaled M. Hosny, Imman M. Mahier, Waleed Y. Rizq, Awaji Y. Safhi, Deena M. Bukhary, Muhammad H. Sultan, Haitham A. Bukhary, Osama A. Madkhali, Fahad Y. Sabei
Summary: Using experimental design, tamsulosin, saw palmetto oil, and pumpkin seed oil were combined in a nanostructured lipid carrier to enhance the permeation and therapeutic activity of tamsulosin. The optimized formulation showed improved drug release profile and pharmacokinetic profile, and demonstrated favorable therapeutic efficacy in treating benign prostatic hyperplasia.
Article
Chemistry, Medicinal
Farah Alsaadi, Alaa H. Falemban, Safaa M. Alsanosi, Deena Bukhary, Yosra Al-Hindi
Summary: This study aimed to investigate the knowledge, attitude, and practice of pregnant females towards COVID-19 vaccine in Makkah Mukarramah. The results showed that the majority of pregnant women had sufficient knowledge of COVID-19 and held a positive attitude towards the disease and preventive measures. However, 39% of the participants believed that hospitals were highly infected with the virus. Therefore, community education is necessary to reduce anxiety and improve vaccine acceptance among pregnant women.
INTERNATIONAL JOURNAL OF LIFE SCIENCE AND PHARMA RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Raed Felimban, Hossam H. Tayeb, Adeel G. Chaudhary, Majed A. Felemban, Fuad H. Alnadwi, Sarah A. Ali, Jazia A. Alblowi, Eman ALfayez, Deena Bukhary, Mohammed Alissa, Safa H. Qahl
Summary: This study aimed to develop nanostructured lipid carriers (NLCs) containing pitavastatin (PV) combined with the essential oil of Pinus densiflora leaf (Pd oil) and assess their cytotoxicity against human gingival fibroblasts (HGF-1). The optimized PV-Pd-NLCs showed good dissolution efficiency and acceptable rheological behavior in a Carbopol 940(R) gel base, and exhibited the best cytotoxic activity against HGF-1 cells compared to other formulations. These findings suggest that the gel containing PV-Pd-NLCs has potential benefits for local treatment of gingival cancer.
Article
Chemistry, Multidisciplinary
Richard d'Arcy, Farah El Mohtadi, Nora Francini, Carlisle R. DeJulius, Hyunmoon Back, Arianna Gennari, Mike Geven, Maria Lopez-Cavestany, Zulfiye Yesim Turhan, Fang Yu, Jong Bong Lee, Michael R. King, Leonid Kagan, Craig L. Duvall, Nicola Tirelli
Summary: This study developed an 'active-stealth' polymer, PTGG, which is less immunogenic in human plasma than PEG, has antioxidant and anti-inflammatory properties, and better protects proteins from denaturation. Experimental results showed that PTGG-protein conjugates were superior to PEG analogues in terms of activity and stability.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Medicinal
Nyla Ajaz, Anum Abbas, Rabia Afshan, Muhammad Irfan, Syed Haroon Khalid, Sajid Asghar, Muhammad Usman Munir, Waleed Y. Y. Rizg, Kamlah Ali Majrashi, Sameer Alshehri, Mohammed Alissa, Mohammed Majrashi, Deena M. M. Bukhary, Ghulam Hussain, Fauzia Rehman, Ikram Ullah Khan
Summary: In this study, a polymeric hydrogel was successfully developed for targeted release of dexamethasone sodium phosphate in the colonic environment. The hydrogel exhibited good swelling and mechanical properties, as well as biocompatibility. Therefore, it holds promise as a potential carrier for treating inflammatory bowel diseases.
Review
Engineering, Biomedical
Filip Hrncirik, Iwan Roberts, Ilkem Sevgili, Chloe Swords, Manohar Bance
Summary: As the first clinically translated machine-neural interface, cochlear implants (CI) have shown success in restoring hearing for severe to profound hearing loss patients. However, challenges such as hearing damage from insertion forces during implantation and limited focusing ability due to current spread hinder wider CI eligibility. This review provides an overview of cochlear anatomy and physical properties as a resource to improve accurate models for future CI treatments. It also discusses the advancements, challenges, and future directions in the development of physical, animal, tissue engineering, and computational models of the cochlea.
ANNALS OF BIOMEDICAL ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Zulfiye Y. Turhan, Richard d'Arcy, Farah El Mohtadi, Lorena Infante Teixeira, Nora Francini, Mike Geven, Valentina Castagnola, Aws Alshamsan, Fabio Benfenati, Nicola Tirelli
Summary: This study describes a family of well-defined block copolymers with oxidative and temperature responses, which exhibit anti-inflammatory effects and aggregation capabilities. However, the detrimental effects on cell viability should be considered. The evaluation suggests that single-responsive controls are needed for a precise assessment of the (synergic or antagonist) potential of double-responsive systems.
Article
Biochemical Research Methods
Hazim M. Ali, Ibrahim Hotan Alsohaimi, Tamer H. A. Hasanin, Afnan S. Batubara, Deena M. Bukhary, Ehab M. Mostafa, Mohammed Gamal
Summary: An ultrasensitive and highly efficient liquid chromatography-mass/mass spectrometric (LC-MS/MS) method was developed for the simultaneous assessment of hyoscine N-butyl bromide (HBB) and ketoprofen (KTP) in biological fluids and pharmaceuticals. The method addressed the challenges of HBB elution difficulty and the presence of KTP as a racemic mixture. Excellent correlation coefficients were obtained for both HBB and KTP, with validation results showing high extraction recoveries and low relative standard deviations.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Article
Biochemistry & Molecular Biology
Umber Zaman, Khalil ur Rehman, Shahid Ullah Khan, Syed Badshah, Khaled M. Hosny, Majd A. Alghamdi, Hatem K. Hmid, Mohammed Alissa, Deena M. Bukhary, Ehab A. Abdelrahman
Summary: The aim of this study was to purify and analyze a thermostable serine alkaline protease from P. minor. The enzyme was purified 2.7-fold with a 12.4% recovery using chromatography and precipitation techniques. The purified protease showed high thermostability and superior kinetic and thermodynamic properties compared to other sources and previously described plants.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Biomedical
Simone R. de Rijk, Alexander J. Boys, Iwan V. Roberts, Chen Jiang, Charlotte Garcia, Roisin M. Owens, Manohar Bance
Summary: Cochlear implants are revolutionary devices that partially restore hearing for those with severe sensorineural hearing loss. However, they can lead to fibrotic tissue formation in the cochlea, causing residual hearing loss and suboptimal outcomes. This study develops a tissue-engineered model to examine the electrical characteristics associated with fibrotic tissue formation.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Biochemistry & Molecular Biology
Maria Balk, Pietro Sofia, Axel T. T. Neffe, Nicola Tirelli
Summary: This review primarily focuses on two aspects: firstly, understanding lignin in terms of its source and production in plants, and the details of its coupling reactions; secondly, exploring the use of lignin as a component in healthcare and energy applications, with specific attention given to its role in the development of organic shape-memory materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Richard d'Arcy, Nicola Tirelli
Summary: Cross-linked micelles made of amphiphilic poly(ethylene glycol)-polysulfide block copolymers act as effective anti-inflammatory agents by scavenging ROS and inhibiting the production of inflammatory cytokines. Compared to self-assembled micelles, cross-linked micelles exhibit better stability, lower toxicity, and less damage to cells.
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2023)
Article
Materials Science, Biomaterials
Alessandro Siani, Lorena Infante-Teixeira, Richard dArcy, Iwan V. Roberts, Farah El Mohtadi, Roberto Donno, Nicola Tirelli
Summary: This paper investigates the effects of reactive oxygen species (ROS) and their removal through nanoparticle-mediated extracellular means on the differentiation of fibroblasts to myofibroblasts induced by TGF-I31. The results demonstrate that poly(propylene sulfide) (PPS) nanoparticles can inhibit ROS-mediated molecular events and prevent the upregulation of key biomechanical elements induced by TGF-I31, suggesting their potential as anti-fibrotic agents.
BIOMATERIALS ADVANCES
(2023)
Article
Polymer Science
Barkat Ali Khan, Sajeel Ahmad, Muhammad Khalid Khan, Khaled M. Hosny, Deena M. Bukhary, Haroon Iqbal, Samar S. Murshid, Abdulrahman A. Halwani, Mohammed Alissa, Farid Menaa
Summary: The aim of this study was to develop a transdermal emulgel for improved analgesic and anti-inflammatory effects, and to reduce adverse reactions in the gastrointestinal tract. The emulgel showed good physical properties, thermodynamic stability, and exhibited effective drug release and permeation. The optimized emulgel demonstrated synergistic effects of naproxen and eugenol in suppressing inflammation and relieving pain.
Article
Medicine, General & Internal
Jumana Sako, Ghdi Almsaoudi, Rasha AL-Malki, Raghidah Alilyyani, Rawan Almatrafi, Raghad Almatrafi, Nouf Alwafi, Shuruq Alamer, Rehab Almehmadi, Reem Alotaibi, Safaa Alsanosi, Alaa Falemban, Deena Bukhary, Yosra Al-Hindi
Summary: This study assesses the awareness level of female pharmacy students in Umm Al-Qura University, Saudi Arabia, about breast cancer. The results show a sufficient level of awareness among future female pharmacists.
WORLD FAMILY MEDICINE
(2022)