Study of pre-formulation and development of solid lipid nanoparticles containing perillyl alcohol

The aim of this work was to study the physicochemical characterization of lipid matrices composed of cetyl palmitate (CP)/perillyl alcohol (PA) and develop preliminary studies of SLN for potential application in cancer treatment. The pre-formulation was submitted to recrystallization process (heating-cooling) in a concentration of CP/AP (F1 1:0; F2 1:9; F3 1:3; and F4 1:1). The characterization showed TG presents a single stage of thermal decomposition for AP, F1, F2 and F3 and two stages for F4. DSC decrease of the onset temperature of CP before and after thermal decomposition in all samples indicates a reduction in crystallinity, and WAXD data confirmed DSC results, showing a reduction in the intensity of main diffraction peaks of the lipid mixtures and a presence of the amorphous portion in 2 theta angles of 21.51 degrees and 23.62 degrees. Results showed the binary mixtures presented a amorphous portion facilitates AP incorporation in SLN satisfactory size, polydispersity index, zeta potential and morphology was obtained.