Article
Cell & Tissue Engineering
Liang-liang Huang, Jun-hong Deng, Jing-xuan Xie, Zi-bin Lin, Hui Jiang, Bin Ouyang, Jian-ming Liu, Yan-ni Wei, Zhou-da Cai
Summary: High cell density promotes the differentiation of USCs into SMCs, with ILK playing a key regulatory role and the NF-kappa B signaling pathway likely playing a crucial role in this process.
STEM CELLS INTERNATIONAL
(2021)
Article
Biotechnology & Applied Microbiology
Haifeng Chen, Mingming Cheng, Pengcheng Gao, Xiangzhong Zhang, Ganggang Li, Liting Wang, Long Qin, Hongrui Li
Summary: GDC-0941 inhibits the survival rate of breast cancer cells, decreases phosphorylation of AKT, S6, and p70S6K, and promotes ILK expression. GDC-0941 affects the sensitivity of breast cancer cells to GDC-0941 by increasing ILK level through upregulation of TNF-alpha.
Article
Cell Biology
Ilenia Masi, Valentina Caprara, Francesca Spadaro, Lidia Chellini, Rosanna Sestito, Andrea Zancla, Alberto Rainer, Anna Bagnato, Laura Rosano
Summary: The study reveals that the endothelin A receptor (ETAR) drives invadopodia through beta-arr1, and high EDNRNILK expression is associated with poor clinical outcomes in serous ovarian cancer (SOC).
Article
Oncology
Paul C. McDonald, Shoukat Dedhar
Summary: Cancer metastasis, involving the spread of cancer cells from the primary tumor to other parts of the body, is a major cause of mortality in cancer patients. Integrin Linked Kinase (ILK) plays a crucial role in promoting physical changes essential for cell motility and invasion during metastasis. Recent advancements have highlighted ILK's involvement in signaling pathways related to tumor progression, metastasis, and therapeutic resistance, making it a potential target for novel cancer treatments. Pharmacological inhibition of ILK activity shows promise in reducing drug resistance and enhancing therapeutic efficacy in combating the metastatic process.
Article
Biochemistry & Molecular Biology
Javier Garcia-Marin, Diego Rodriguez-Puyol, Juan J. Vaquero
Summary: Pseudokinases play a role in intracellular signaling despite their lack of catalytic activity. The study shows that the V-shaped pyrazole Cpd22 directly binds to integrin-linked kinase (ILK) and stabilizes its domain, possibly modulating its scaffolding properties as a pseudokinase. These findings provide a basis for the development of new chemical probes and potential drugs.
JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN
(2022)
Article
Multidisciplinary Sciences
Xinlan Qu, Yuan Fang, Siying Zhuang, Yuanzhen Zhang
Summary: The study demonstrates that miR-542-3p can affect the differentiation of HESCs by down-regulating ILK, inhibiting angiogenesis, reducing tumor growth and invasion, and inducing apoptosis.
SCIENTIFIC REPORTS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Chuan He, Tairan Wang, Yijie Wang, Tongtong Xu, Shuyang Zhao, Haoyu Shi, Rui Zou
Summary: Mechanical memory exists in human periodontal ligament stem cells, and ILK plays a significant role in this process.
Review
Biochemistry & Molecular Biology
Agata Gorska, Antonina Joanna Mazur
Summary: Integrin-linked kinase (ILK) plays a crucial role in important cellular processes, such as cell adhesion, cell growth, and systemic changes. However, the exact mechanisms and functions of ILK are still not fully understood. Despite the existence of multiple ILK isoforms, research tends to focus on the main isoform, while the functional differences of other isoforms are still unclear. ILK activity is regulated at multiple levels, but the functions of most modifications are unknown.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Galina S. Baturina, Liubov E. Katkova, Claus Peter Schmitt, Evgeniy Solenov, Sotirios G. Zarogiannis
Summary: In this study, it was found that under hyperosmolar isotonic conditions, primary cultures of rat peritoneal mesothelial cells and rat kidney outer medullary collecting duct (OMCD) principal cells exhibit different cell volume regulation mechanisms in response to urea and myo-inositol. Peritoneal mesothelial cells show regulatory volume decrease (RVD) activation when exposed to isotonic urea inflow, while kidney OMCD cells demonstrate volume restoration with urea inflow. Additionally, both cell types display a significant increase in intracellular calcium in response to isotonic application of the osmolytes.
Article
Chemistry, Medicinal
Javier Garcia-Marin, Mercedes Griera-Merino, Alejandra Matamoros-Recio, Sergio de Frutos, Manuel Rodriguez-Puyol, Ramon Alajarin, Juan J. Vaquero, Diego Rodriguez-Puyol
Summary: ILK is a controversial pseudokinase protein that plays a crucial role in integrin-mediated signaling and serves as a scaffolding protein inside cells, linked to non-neoplastic diseases like CKD. By designing new tripeptide derivatives, compounds with ILK activity can be generated, offering potential for new drug research.
ACS MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Pharmacology & Pharmacy
Wei Zheng, Tao Huang, Qi-Zhen Tang, Shi Li, Jie Qin, Feng Chen
Summary: Astragalus polysaccharide extract (APS) has been shown to significantly reduce blood pressure in hypertensive mice, reduce inflammation and renal fibrosis, and improve renal function. In addition, APS administration can alleviate the increase in serum creatinine, urea nitrogen, and uric acid levels.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Sport Sciences
Georgios Mavropalias, Yu-Fu Wu, Marni D. Boppart, Anthony J. Blazevich, Kazunori Nosaka
Summary: This study demonstrates that eccentric cycling training can increase muscle mass, strength, and power, as well as the levels of integrin-beta 1 and RICTOR protein and p-ILK/t-ILK, which may play a role in protecting against muscle damage and inducing muscle adaptations.
MEDICINE & SCIENCE IN SPORTS & EXERCISE
(2022)
Article
Biochemistry & Molecular Biology
Dorota Gil, Piotr Laidler, Marta Zarzycka, Joanna Dulinska-Litewka
Summary: The study highlights the dual role of autophagy in cancer and the potential therapeutic implications. Knockdown of ILK was found to promote autophagy through AMPK activation, leading to suppression of melanoma cell growth and induction of apoptosis. Combining CQ and siILK showed stronger antitumor effects, possibly through synergistic inhibition of protein synthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Ewa Mrowczynska, Antonina Joanna Mazur
Summary: This study confirms the expression of ILK in chicken DRG and its regulatory role in DRG development, demonstrating its crucial functions in DRG neurons and SCPs through functional experiments.
Article
Pharmacology & Pharmacy
Arnab Datta, Chin-Rang Yang, Karim Salhadar, Euijung Park, Chung-Lin Chou, Viswanathan Raghuram, Mark A. Knepper
Summary: This study systematically analyzed vasopressin-induced phosphorylation changes and identified at least six downstream protein kinases that are regulated by vasopressin, providing important insights for future research.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Editorial Material
Rheumatology
Soichiro Sugitani, Kenji Tsuji, Toshio Yamanari, Yoichi Hasegawa, Kentaro Inoue, Yuka Sogabe, Yukari Nakano, Shinji Kitamura, Tsutomu Ishizu, Jun Wada
JCR-JOURNAL OF CLINICAL RHEUMATOLOGY
(2021)
Article
Medicine, General & Internal
Shinji Kitamura, Kensaku Takahashi, Yizhen Sang, Kazuhiko Fukushima, Kenji Tsuji, Jun Wada
Review
Biochemistry & Molecular Biology
Kenji Tsuji, Shinji Kitamura, Jun Wada
Summary: Nephrotic syndrome is a clinical situation caused by a variety of diseases, with renal biopsy being the best diagnostic option currently available. MiRNAs in serum and urine have been proven to be non-invasive biomarkers in multiple diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Letter
Urology & Nephrology
Takato Nakadoi, Kenji Tsuji, Takehiro Iwata, Eriko Eto, Hisashi Masuyama, Koji Tomita, Takao Hiraki, Shinji Kitamura, Hitoshi Sugiyama, Jun Wada
Article
Urology & Nephrology
Api Chewcharat, Elizabeth A. Phipps, Khushboo Bhatia, Sahir Kalim, Andrew S. Allegretti, Meghan E. Sise, Teodor G. Paunescu, Rituvanthikaa Seethapathy, Sagar U. Nigwekar
Summary: The prevalence of olfactory dysfunction is higher in patients with chronic kidney disease (CKD), while there is no significant association between CKD and gustatory dysfunction. Decreased grip strength is associated with higher odds of olfactory dysfunction in CKD patients.
Article
Endocrinology & Metabolism
Natsumi Matsuoka-Uchiyama, Haruhito A. Uchida, Shugo Okamoto, Yasuhiro Onishi, Katsuyoshi Katayama, Mariko Tsuchida-Nishiwaki, Hidemi Takeuchi, Rika Takemoto, Yoshiko Hada, Ryoko Umebayashi, Naoko Kurooka, Kenji Tsuji, Jun Eguchi, Hirofumi Nakajima, Kenichi Shikata, Jun Wada
Summary: This study found that variability in postprandial triglyceride levels is associated with the exacerbation of diabetic kidney disease, making it a novel risk factor.
JOURNAL OF DIABETES RESEARCH
(2022)
Article
Medicine, General & Internal
Kazuhiko Fukushima, Haruhito A. Uchida, Yasuko Fuchimoto, Tomoyo Mifune, Mayu Watanabe, Kenji Tsuji, Katsuyuki Tanabe, Masaru Kinomura, Shinji Kitamura, Yosuke Miyamoto, Sae Wada, Taisaku Koyanagi, Hitoshi Sugiyama, Takumi Kishimoto, Jun Wada
Summary: Close attention is needed, particularly for respiratory system events and infectious diseases, when treating patients with silica-associated SLE.
Review
Otorhinolaryngology
Zara M. Patel, Eric H. Holbrook, Justin H. Turner, Nithin D. Adappa, Mark W. Albers, Aytug Altundag, Simone Appenzeller, Richard M. Costanzo, Ilona Croy, Greg E. Davis, Puya Dehgani-Mobaraki, Richard L. Doty, Valerie B. Duffy, Bradley J. Goldstein, David A. Gudis, Antje Haehner, Thomas S. Higgins, Claire Hopkins, Caroline Huart, Thomas Hummel, Kawinyarat Jitaroon, Robert C. Kern, Ashoke R. Khanwalkar, Masayoshi Kobayashi, Kenji Kondo, Andrew P. Lane, Matt Lechner, Donald A. Leopold, Joshua M. Levy, Michael J. Marmura, Lisha Mclelland, Takaki Miwa, Paul J. Moberg, Christian A. Mueller, Sagar U. Nigwekar, Erin K. O'Brien, Teodor G. Paunescu, Robert Pellegrino, Carl Philpott, Jayant M. Pinto, Evan R. Reiter, David R. Roalf, Nicholas R. Rowan, Rodney J. Schlosser, James Schwob, Allen M. Seiden, Timothy L. Smith, Zachary M. Soler, Leigh Sowerby, Bruce K. Tan, Andrew Thamboo, Bozena Wrobel, Carol H. Yan
Summary: This article reviews the literature on clinical olfaction, olfactory loss, and olfactory dysfunction over the past two decades and introduces the International Consensus on Allergy and Rhinology: Olfaction (ICAR:O). Nearly 100 topics were thoroughly reviewed, providing important insights and clear guidance for the diagnosis and treatment of patients, while also highlighting gaps in our knowledge and evidence base.
INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY
(2022)
Review
Cell Biology
Kenji Tsuji, Shinji Kitamura, Jun Wada
Summary: Kidney diseases are a global health problem and stem cells offer potential for kidney regeneration. There are two main strategies for kidney regeneration: de novo whole kidney fabrication and stem cell therapy. These strategies have shown significant progress over the past few decades, but there are still challenges for their clinical application.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Urology & Nephrology
Kenji Tsuji, Shinji Kitamura, Haruhito A. Uchida, Jun Wada
Summary: Real-time ultrasound-guided renal biopsy is a commonly used technique for diagnosing various kidney diseases. To reduce the risks of complications, we developed a simple simulation tool using boiled eggs as a model for kidney biopsies. The feedback from participants, including trainees and experienced nephrologists, was positive, with all trainees agreeing that the simulation reduced their fears of performing renal biopsies. All trainees and nephrologists recommended using the simulator for training before conducting biopsies on actual patients.
Article
Medicine, Research & Experimental
Sudhir Kumar, Xueping Fan, Hila Milo Rasouly, Richa Sharma, David J. Salant, Weining Lu
Summary: This study identifies the key role of ZEB2 in maintaining the cell fate of FOXD1(+) stromal progenitors during kidney development. Loss of ZEB2 leads to differentiation of FOXD1(+) stromal progenitors into myofibroblasts and kidney fibrosis.
Article
Medicine, General & Internal
Natsumi Matsuoka-Uchiyama, Kenji Tsuji, Kensaku Takahashi, Kazuhiko Fukushima, Hidemi Takeuchi, Shinji Kitamura, Kenichi Inagaki, Haruhito A. A. Uchida, Jun Wada
Summary: This study examined the association between urinary creatinine excretion rate (CER) and hypothyroidism. The results showed that hypothyroidism was not independently associated with CER, and estimated glomerular filtration rate calculated using serum creatinine (eGFRcre) is a useful marker for evaluating kidney function regardless of hypothyroidism.
Review
Medicine, General & Internal
Yizhen Sang, Kenji Tsuji, Hiroyuki Nakanoh, Kazuhiko Fukushima, Shinji Kitamura, Jun Wada
Summary: Kidney diseases are a global public health issue with limited treatment options. Semaphorin 3A (SEMA3A) plays a crucial role in kidney pathophysiology, with excess expression worsening kidney injury and inhibition of SEMA3A potentially ameliorating kidney damage, making it a potential therapeutic target for kidney diseases.
Review
Medicine, General & Internal
Kenji Tsuji, Hiroyuki Nakanoh, Kazuhiko Fukushima, Shinji Kitamura, Jun Wada
Summary: Acute kidney injury (AKI) is a serious clinical syndrome characterized by a decrease in urine output and kidney function, leading to electrolyte and acid-base imbalances. Early diagnosis and treatment are crucial, and miRNAs have shown potential as biomarkers and therapeutic targets for AKI.
Article
Physiology
Yizhen Sang, Kenji Tsuji, Kazuhiko Fukushima, Kensaku Takahashi, Shinji Kitamura, Jun Wada
Summary: Renal fibrosis, a common pathological process in renal diseases, was studied using a UUO mouse model, revealing increased SEMA3A signaling and its inhibition ameliorating fibrosis through JNK regulation. This study suggests SEMA3A inhibitor as a potential therapeutic option for renal fibrosis.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)