Journal
BIOPHYSICAL CHEMISTRY
Volume 211, Issue -, Pages 19-27Publisher
ELSEVIER
DOI: 10.1016/j.bpc.2016.01.002
Keywords
beta(2)-Microglobulin; Antigen-antibody biorecognition; AFM; Atomic Force Spectroscopy; Surface Plasmon Resonance
Funding
- Italian Association for Cancer Research (AIRC) [IG 10412]
- PRIN-MIUR Project [2012NRRP5J]
Ask authors/readers for more resources
beta(2)-Microglobulin (B2M) is a human protein involved in the regulation of immune response and represents a useful biomarker for several diseases. Recently, anti-B2M monoclonal antibodies have been introduced as innovative therapeutic agents. A deeper understanding of the molecular interaction between the two partners could be of utmost relevance for both designing array-based analytical devices and improving current immunotherapies. A visualization at the nanoscale performed by Atomic Force Microscopy revealed that binding of B2M to the antibody occurred according to two preferred interaction geometries. Additionally, Atomic Force Spectroscopy and Surface Plasmon Resonance provided us with detailed information on the binding kinetics and the energy landscape of the complex, both at the single molecule level and in bulk conditions. Combination of these complementary techniques contributed to highlight subtle differences in the kinetics behaviour characterizing the complexes. Collectively, the results may deserve significant interest for designing, development and optimization of novel generations of nanobiosensor platforms. (C) 2016 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available