Article
Materials Science, Multidisciplinary
Jose M. de Hoyos-Vega, Hye Jin Hong, Kevin Loutherback, Gulnaz Stybayeva, Alexander Revzin
Summary: Liver cultures have evolved from hepatocyte monocultures to more complex co-cultures and precision-cut liver slices. This study presents a microfluidic device that allows for longer-term maintenance of functional organotypic liver cultures, displaying promising potential for disease modeling and individualized therapies.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Clinical Neurology
Jakob Matschke, Henri Lahann, Susanne Krasemann, Hermann Altmeppen, Susanne Pfefferle, Giovanna Galliciotti, Antonia Fitzek, Jan-Peter Sperhake, Benjamin Ondruschka, Miriam Busch, Natalie Rotermund, Kristina Schulz, Christian Lohr, Matthias Dottermusch, Markus Glatzel
Summary: The mechanism of how SARS-CoV-2 infection leads to brain damage is still not clear. This study found that the degree of immune cell activation in COVID-19 patients is related to age, while the degree of reactive astrogliosis is age-dependent but not influenced by COVID-19.
FRONTIERS IN NEUROLOGY
(2022)
Article
Transplantation
Benedikt Simon, Harald Rubey, Andreas Treipl, Martin Gromann, Boris Hemedi, Sonja Zehetmayer, Bernhard Kirsch
Summary: HD patients had significantly lower anti-SARS-CoV-2 S antibody titres compared to controls 21 days after vaccination. The HD group experienced fewer adverse events than the control group. There was no correlation between the antibody response to Hepatitis B vaccination and the SARS-CoV-2 vaccine.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2021)
Article
Multidisciplinary Sciences
Birgit Lohberger, Susanne Scheipl, Ellen Heitzer, Franz Quehenberger, Danielle de Jong, Karoly Szuhai, Bernadette Liegl-Atzwanger, Beate Rinner
Summary: Chordomas are rare malignant bone tumors with no approved medical treatment. Research has shown that cMET and HGF are expressed in Chordomas, and cMET inhibitors may benefit patients with sacral Chordomas.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Chris E. Holmquist, Wenxia He, Rita M. Meganck, William F. Marzluff
Summary: Metazoan histone mRNAs, which are not polyadenylated and are tightly regulated, undergo degradation and modification with the involvement of 3'hExo and TUT7.
Article
Cell Biology
Elizabeth M. Anderson, Shuk Hang Li, Moses Awofolaju, Theresa Eilola, Eileen Goodwin, Marcus J. Bolton, Sigrid Gouma, Tomaz B. Manzoni, Philip Hicks, Rishi R. Goel, Mark M. Painter, Sokratis A. Apostolidis, Divij Mathew, Debora Dunbar, Danielle Fiore, Amanda Brock, JoEllen Weaver, John S. Millar, Stephanie DerOhannessian, Allison R. Greenplate, Ian Frank, Daniel J. Rader, E. John Wherry, Paul Bates, Scott E. Hensley
Summary: The study found that SARS-CoV-2 infections and mRNA vaccinations elicit fundamentally different antibody responses. Infection and initial vaccination produce S1 and S2 reactive antibodies, while secondary vaccination mainly enhances S1 antibodies. The research also revealed that infections generate a large proportion of antibodies with efficient binding to the spike protein of common seasonal human coronaviruses but poor binding to the spike protein of SARS-CoV-2, whereas vaccination boosts antibodies that react to the spike protein of common seasonal human coronaviruses and cross-react more efficiently to the spike protein of SARS-CoV-2.
Article
Immunology
Nina Koerber, Laureen Pohl, Birgit Weinberger, Beatrix Grubeck-Loebenstein, Andrea Wawer, Percy A. Knolle, Hedwig Roggendorf, Ulrike Protzer, Tanja Bauer
Summary: This study revealed higher frequencies of regulatory B cells (Breg) and lower IL-10 expression levels in second-generation hepatitis B vaccine non-responders, indicating a potential mechanism for the lack of protective antibody response following vaccination. Additionally, the decrease in Breg numbers after booster immunization with a third-generation hepatitis B vaccine suggests a positive effect of third-generation vaccines on Breg-mediated immunomodulation in non-responders.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Alfredo Santovito, Martina Saracco, Manuel Scarfo, Alessandro Nota, Sandro Bertolino
Summary: In this study, the researchers used a non-invasive approach to investigate the relationship between inbreeding and genomic damage in dogs. They found that inbreeding may increase the levels of genomic damage in purebred dogs. These findings are important for discouraging inbreeding practices and reducing the fragmentation of wild populations.
Article
Agriculture, Dairy & Animal Science
K. S. Akhil, M. Chackochan, C. Sunanda, P. M. Rojan, S. N. Nair, S. Simon, D. Sanjay, C. N. Dinesh, R. Ravindran
Summary: The study evaluated various factors influencing cattle resistance to tick infestation in Kerala State, South India. It found that crossbred cows had higher tick infestation levels than indigenous breeds, and heifers and dry animals were more susceptible than lactating animals. Factors like parity, grazing, and region also influenced tick infestation.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2021)
Article
Rheumatology
Ayse Ozdede, Okan Kadir Nohut, Zeynep Atli, Yesim Tuyji Tok, Sabriye Guner, Erkan Yilmaz, Didar Ucar, Ugur Uygunoglu, Vedat Hamuryudan, Emire Seyahi
Summary: Limited data on humoral response to COVID-19 vaccines in patients with Behcet's syndrome (BS) were studied. The study compared the antibody response to two different vaccines (inactivated or mRNA) in BS patients and healthy controls (HCs). The results showed that the majority of both patient and HC groups had detectable antibodies after vaccination. However, BS patients vaccinated with the inactivated vaccine had significantly lower antibody titers compared to HCs, while there was no difference in antibody titers between BS patients and HCs vaccinated with the mRNA vaccine. The study also found that BS patients using anti-TNF-based treatment had the lowest antibody titers, particularly in the group vaccinated with the inactivated vaccine. Further research is needed to evaluate whether the lower antibody titers are associated with reduced protection against COVID-19 in both vaccine groups.
RHEUMATOLOGY INTERNATIONAL
(2022)
Article
Andrology
Fatemeh Anbari, Mohammad Ali Khalili, Abdul Munaf Sultan Ahamed, Esmat Mangoli, Ali Nabi, Fatemeh Dehghanpour, Mojdeh Sabour
Summary: This study compared sperm quality, DNA integrity, embryo development, and clinical outcomes between sperm samples prepared using Microfluidic Sperm Sorting (MSS) and Direct Swim Up (DSU) methods for intracytoplasmic sperm injection (ICSI). The results showed that MSS significantly improved sperm quality parameters and clinical outcomes compared to DSU.
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
(2021)
Article
Endocrinology & Metabolism
Antonis Polymeris, Petros D. Papapetrou, Stavroula Psachna, Dimitrios Ioannidis, Dimitrios Lilis, Maria Drakou, Aristeidis Vaiopoulos, Vaia Polymerou, Georgios Spanos
Summary: The aim of this study was to evaluate the response to the COVID-19 mRNA vaccine in patients with Hashimoto's thyroiditis compared to normal individuals. The results showed that patients with Hashimoto's thyroiditis exhibited a significantly higher immune response after the third dose of the vaccine compared to normal individuals.
HORMONES-INTERNATIONAL JOURNAL OF ENDOCRINOLOGY AND METABOLISM
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Koichiro Kaneko, Shingo Baba, Takuro Isoda, Hisakazu Ishioka
Summary: The study found that SiPM-based PET/CT scanners show significantly higher arterial F-18-FDG uptake compared to conventional scanners, especially in vessels with a diameter less than 15mm. Additionally, the threshold TBR >= 1.6 is not applicable for the carotid artery in SiPM-based PET/CT systems.
NUCLEAR MEDICINE COMMUNICATIONS
(2021)
Article
Food Science & Technology
Rachid Jellali, Sebastien Jacques, Amal Essaouiba, Francoise Gilard, Franck Letourneur, Bertrand Gakiere, Cecile Legallais, Eric Leclerc
Summary: Several studies have found a correlation between pesticides exposure and metabolic disorders, particularly with DDT and PMT. This study investigated the effects of DDT, PMT, and their mixture on liver damage in rat organ-on-chip models using multi-omics analyses. The results showed dose-dependent effects and identified common metabolic signatures across the treatments, including changes in lipid and carbohydrate production. This research highlights the potential of organ-on-chip technology combined with multi-omics for toxicological studies and chemical risk assessment.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Megane Beldjilali-Labro, Rachid Jellali, Alexander David Brown, Alejandro Garcia Garcia, Augustin Lerebours, Erwann Guenin, Fahmi Bedoui, Murielle Dufresne, Claire Stewart, Jean-Francois Grosset, Cecile Legallais
Summary: The goal of this study was to design and evaluate a multi-scale scaffold for promoting the differentiation of C2C12 skeletal myoblasts. The researchers found that the scaffold had tunable mechanical properties and improved the alignment and length of myotubes, leading to enhanced organization of reconstructed skeletal muscle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Food Science & Technology
Junting Hong, Nadia Boussetta, Gerald Enderlin, Franck Merlier, Nabil Grimi
Summary: Atrazine, a commonly used herbicide, can cause harm to humans if ingested through residual residues in food and water. Therefore, finding clean and cost-effective degradation methods for atrazine is crucial. Various physical, chemical, and biological methods have been proposed to remove atrazine from aquatic environments.
Article
Polymer Science
Leire Etxeberria, Taha Messelmani, Jon Haitz Badiola, Andreu Llobera, Luis Fernandez, Jose Luis Vilas-Vilela, Eric Leclerc, Cecile Legallais, Rachid Jellali, Ane Miren Zaldua
Summary: This study developed a microfluidic device for culturing liver cell line under dynamic conditions, using a process suitable for mass production. The device, made of cyclic olefin copolymers, integrates connectors compatible with standard medical and laboratory instruments. Experimental results showed that the cells proliferated well and remained functional for 9 days of culture in the device, and exhibited similar behavior to cells in traditional material-based biochips. This study provides a proof-of-concept for the use of the developed biochips in liver cell culture and illustrates their potential in advancing OoC technology.
Article
Biochemistry & Molecular Biology
Junting Hong, Nadia Boussetta, Gerald Enderlin, Nabil Grimi, Franck Merlier
Summary: High resolution mass spectrometry coupled with ultra-high-performance liquid chromatography was used to monitor the degradation process of atrazine (ATZ) during Fenton/ultrasound treatment. The online analysis through automatic sampling and rapid detection prevented degradation and improved measurement accuracy.
Article
Biochemistry & Molecular Biology
Amal Essaouiba, Rachid Jellali, Francoise Gilard, Bertrand Gakiere, Teru Okitsu, Cecile Legallais, Yasuyuki Sakai, Eric Leclerc
Summary: Diabetes mellitus is a complex disease with a high prevalence and mortality rate. Advanced in vitro models and analytical tools, such as microfluidic biochips, have been developed to investigate the pancreatic tissue response and identify therapeutic solutions. In this study, rat islets of Langerhans were cultured in microfluidic biochips and compared to static cultures in Petri dishes. The results showed that the biochip culture maintained high secretions of insulin, C-peptide, and glucagon compared to the Petri dish culture. Exometabolomic profiling revealed differential expression of metabolites between the two culture methods, indicating changes in lipid metabolism, the pentose phosphate pathway, and the TCA cycle in the biochip culture. Drug stimulation further perturbed the exometabolome of the biochip culture, with GLP1 leading to an increase in glycolysis and pentose phosphate pathway intermediates, and isradipine causing reduced levels of lipids and carbohydrates.
Article
Biotechnology & Applied Microbiology
Sergio Tomas-Martinez, Erwin J. Zwolsman, Franck Merlier, Martin Pabst, Yuemei Lin, Mark C. M. van Loosdrecht, David G. Weissbrodt
Summary: Polyphosphate accumulating organisms (PAOs) play a crucial role in the removal of phosphate from wastewater. However, little is known about the dynamics of extracellular polymeric substances (EPS) in mixed cultures and their interaction with flanking microbes. Understanding EPS turnover can provide insights into their biosynthesis and degradation cycles.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ye Tao, Yannick Rossez, Clovis Bortolus, Luminita Duma, Faustine Dubar, Franck Merlier
Summary: High-resolution mass spectrometry (HRMS) coupled with ultra-high-performance liquid chromatography (UHPLC) allows for simultaneous quantification of trehalose and trehalose 6-phosphate without derivatization or sample preparation. The use of full scan mode and exact mass analysis enables metabolomic analyses and semi-quantification. The method has been validated for different matrices, yeasts, and bacteria, and can differentiate between bacteria based on growth temperatures.
Article
Pharmacology & Pharmacy
Taha Messelmani, Anne Le Goff, Fabrice Soncin, Francoise Gilard, Zied Souguir, Nathalie Maubon, Bertrand Gakiere, Cecile Legallais, Eric Leclerc, Rachid Jellali
Summary: Organ-on-chip technology is a promising in vitro approach for studying responses to drug exposure. This study used a metabolomic investigation to analyze a coculture of liver sinusoidal endothelial cells (LSECs) with hepatocytes using advanced organ-on-chip technology. The results demonstrated the potential of a metabolomic-on-chip strategy for pharmaco-metabolomic applications.
Article
Nanoscience & Nanotechnology
Taha Messelmani, Isabela Zarpellon Nascimento, Eric Leclerc, Cecile Legallais, Adam Meziane, William Cesar, Rachid Jellali, Anne Le Goff
Summary: This study investigates the flow rate through a biochip using a peristaltic pump and reservoirs with liquid/air interfaces, both analytically and experimentally. The results show that, under slow changes in hydraulic resistance, the system can reach an equilibrium state, and guidelines are provided for designing tubings and reservoirs.
MICROFLUIDICS AND NANOFLUIDICS
(2023)
Article
Toxicology
Sabrina Madiedo-Podvrsan, Louise Sebillet, Thomas Martinez, Salimata Bacari, Fengping Zhu, Marie Cattelin, Eric Leclerc, Franck Merlier, Rachid Jellali, Ghislaine Lacroix, Muriel Vayssade
Summary: An alternative in vitro method was developed to study systemic-like responses to inhalation-like exposures, replacing animal experimentation. A coculture platform was established to emulate inter-organ crosstalks between a pulmonary barrier and the liver. The coculture model successfully emulated active and functional in vitro crosstalk, responsive to xenobiotic exposure doses.
TOXICOLOGY IN VITRO
(2023)
Article
Biotechnology & Applied Microbiology
Taha Messelmani, Anne Le Goff, Zied Souguir, Victoria Maes, Meryl Roudaut, Elodie Vandenhaute, Nathalie Maubon, Cecile Legallais, Eric Leclerc, Rachid Jellali
Summary: This study investigates the development of hepatic cells in a 3D hydroscaffold inside a microfluidic device, aiming to achieve relevant in vivo-like functionalities of liver tissue. The results demonstrate the formation of 3D aggregates and spheroids with high cell viability and functionality. This liver-on-chip model coupled with a hydroscaffold has the potential to serve as a relevant liver model for drug screening and disease study.
BIOENGINEERING-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Amal Essaouiba, Rachid Jellali, Stephane Poulain, Fumiya Tokito, Francoise Gilard, Bertrand Gakiere, Soo Hyeon Kim, Cecile Legallais, Yasuyuki Sakai, Eric Leclerc
Summary: Functional differentiation of pancreatic-like tissue from human induced pluripotent stem cells can be achieved by culturing hiPSC-derived beta-like cells in spheroids using microfluidic technologies. This study successfully developed spheroids containing cells positive to insulin and glucagon, and further analysis revealed the activation of key transcription factors and upregulation of genes involved in pancreatic tissue development, extracellular matrix remodeling, lipid and fatty acid metabolism, and endocrine hormone signaling. The dynamic 3D biochip cultures showed enhanced activity of certain transcription factors and increased secretion of specific metabolites.
Review
Biochemical Research Methods
Taha Messelmani, Lisa Morisseau, Yasuyuki Sakai, Cecile Legallais, Anne Le Goff, Eric Leclerc, Rachid Jellali
Summary: The liver is a key organ that plays a crucial role in metabolism and has various functions in the body. Most compounds, such as drugs and pollutants, can cause hepatotoxicity and lead to liver damage. Animal models and 2D cell cultures are commonly used to study the effects of chemicals on the liver, but they have limitations. The development of liver organ-on-chip technology provides a promising approach for more relevant toxicology studies.
Article
Nanoscience & Nanotechnology
Lilandra Boulais, Rachid Jellali, Ulysse Pereira, Eric Leclerc, Sidi A. Bencherif, Cecile Legallais
Summary: A biochip integrated with alginate-based cryogel was engineered for dynamic hepatoma cell line culture in 3D, showing high mechanical stability and efficient cell growth. This study represents a proof of concept for further investigation in engineering healthy and cirrhotic liver models.
ACS APPLIED BIO MATERIALS
(2021)
