Article
Food Science & Technology
Fuat Aydin, Tuba Kayman, Secil Abay, Harun Hizlisoy, Izzet Burcin Saticioglu, Emre Karakaya, Orhan Sahin
Summary: This study investigated the genetic diversity, prevalence, and mechanisms of resistance to quinolone antibiotics in 178 Campylobacter jejuni isolates from humans, cattle, dogs, and chickens in Turkey. The results showed that there were 21 clonal complexes (CCs) and 78 different sequence types (STs) among the isolates, with CC21, CC353, CC206, and CC257 being the predominant clones. Quinolone resistance was detected in 41% of the isolates, and all resistant isolates had a specific mutation in the gyrA gene. The study provided important insights into the transmission pathways of C. jejuni in Turkey, suggesting that broiler meat and dogs may be the most important sources of human campylobacteriosis in the country.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Microbiology
Yunyi Zhang, Xuan Deng, Yan Jiang, Junyan Zhang, Li Zhan, Lingling Mei, Hangjing Lu, Pingping Yao, Hanqing He
Summary: From 2011 to July 2021, there were 55 reported cases of Neisseria meningitidis in Zhejiang Province, China. The predominant serogroup was MenB, with an increasing incidence over the years. This study provided valuable data on the temporal epidemiological characteristics of meningococcal disease and healthy carriage.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Chien-Shun Chiou, Ying-Shu Liao, Bo-Han Chen, Min-Chi Lu, Yu-Ping Hong, You-Wun Wang, Ru-Hsiou Teng
Summary: This study presents the demographic and genetic characteristics of invasive meningococcal disease (IMD) in Taiwan. The results show that IMD is rare in Taiwan, with NmB being the predominant serogroup responsible for the majority of cases. The study also highlights the effectiveness of cgMLST in comparing genetic relatedness among a large number of isolates.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Yizhun Li, Yamei Li, Leshan Xiu, Yaling Zeng, Chi Zhang, Liying Sun, LuLu Zhang, Feng Wang, Junping Peng
Summary: This study described the molecular epidemiology of antimicrobial resistance and the distribution of resistance-associated genotypes in Neisseria gonorrhoeae in Shenzhen, China from 2014 to 2018. Typing schemes revealed specific genotypes associated with decreased susceptibility to ceftriaxone, with certain sequence types showing international spread.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Stefano Ravaioli, Davide Campoccia, Werner Ruppitsch, Franz Allerberger, Alessandro Poggi, Emanuele Chisari, Lucio Montanaro, Carla Renata Arciola
Summary: This study evaluated the usefulness of automated ribotyping as a screening tool for identifying S. aureus genetic lineages. Results showed that automated ribotyping can predict spa and MLST clonal complexes, but its high cost and lower discriminatory power could be obstacles for fine genotyping analysis. However, automated ribotyping has advantages such as automation, ease of execution, stability, typeability, and reproducibility, making it a reliable method to be used alongside gold standard methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Maria Giufre, Elena Mazzolini, Marina Cerquetti, Silvio Brusaferro
Summary: This study compared ESBL-producing Escherichia coli isolates from humans and food-producing animals, finding different types of ESBLs in different sources. The results suggest the potential exchange of ESBL genes from animals to humans, emphasizing the need for strict monitoring based on a 'One Health' approach.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Ophthalmology
Yin-Hsi Chang, Yhu-Chering Huang, Hung-Chi Chen, David H. K. Ma, Lung-Kun Yeh, Kuo-Hsuan Hung, Ching-Hsi Hsiao
Summary: This study investigated the molecular and phenotypic characteristics of methicillin-resistant Staphylococcus epidermidis (MRSE) isolated from healthy conjunctiva and ocular infections. The results showed that MRSE isolates from both sources exhibited overlapping molecular types and phenotypic traits. Specific strain ST59 showed distinctive characterizations compared to other strains.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Microbiology
Jose Paulo H. Sato, Amanda G. S. Daniel, Carlos A. G. Leal, David E. S. N. Barcellos, Roberto M. C. Guedes
Summary: This study evaluated the genetic diversity, distribution, evolution, and population structure of Brazilian Brachyspira hyodysenteriae strains. The results showed genetic relationships between the strains from different regions in Brazil and their relation to global isolates.
VETERINARY MICROBIOLOGY
(2022)
Article
Mycology
Immaculata Xess, Mragnayani Pandey, Yubhisha Dabas, Reshu Agarwal, Shukla Das, Padma M. Srivastava, Rajeev Thakur, Shyama Sharma, Prashant Mani, Ashutosh Biswas, Dipankar Bhowmik, Bimal K. Das, Rakesh Singh, Preetilata Panda, Gagandeep Singh, A. C. Phukan, Margaret Yhome, Chithra Valsan, Anupma Jyoti Kindo
Summary: The study on Cryptococcosis in India utilized multilocus sequence typing to analyze 208 clinical isolates of Cryptococcus, revealing 10 Sequence Types (STs) with a majority belonging to molecular type VNI for C. neoformans. The study also found less diversity among Indian isolates compared to the global MLST database.
Article
Microbiology
Shlomo Pilo, Gal Zizelski Valenci, Mor Rubinstein, Lea Pichadze, Yael Scharf, Zeev Dveyrin, Efrat Rorman, Israel Nissan
Summary: This study improved Bom's MLST scheme by using newly designed primers and stringent conditions, enhancing the detection performance for samples with low concentrations of Chlamydia DNA. A simple scoring scheme and flow chart were developed based on Real-Time PCR analysis values to identify reaction inhibitors and predict amplification success. The new MLST version showed great results in patients attending an STI clinic.
Article
Microbiology
Jia xin Zhong, Hao ran Zheng, Yuan yuan Wang, Lu lu Bai, Xiao li Du, Yuan Wu, Jin xing Lu
Summary: This study investigated the molecular types, antimicrobial resistance profiles, and resistance genes of Clostridium perfringens from human, animal, and food sources. The most prevalent molecular types of C. perfringens in humans were ST221, ST62, ST408, and ST493, while ST479 was the major type in animals. Analysis using cgMLST and cgSNP revealed genetic relatedness and transmission events. The study provides an overview of the epidemiological characteristics of C. perfringens with different origins and hosts in China.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Pedro Vallejo, Gustavo Cilla, Maddi Lopez-Olaizola, Diego Vicente, Jose Maria Marimon
Summary: This study described the clinical features and molecular epidemiology of human listeriosis over an 11-year period in Gipuzkoa, Northern Spain. The study found that listeriosis had an average annual incidence of 1.55 per 100,000 population in non-pregnancy-associated cases, with a 1-month mortality rate of 22.2%. In pregnant women, the average incidence was 0.45 cases per 1,000 pregnancies.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Yang Qu, Chao Wei, Xiaohang Dai, Yalong Bai, Xin Zhao, Qingkuo Lan, Wenbo Wang, Yuanjuan Wu, Min Gao, Weihao Tang, Changyan Zhou, Yujuan Suo
Summary: A study in China found a potential transmission of Bacillus cereus and its enterotoxicity on lettuce farms, with high contamination rates mainly coming from soil and pesticides. The results highlight potential health risks posed by B. cereus isolates from lettuce and the environment.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Mark E. Berrang, Elizabeth A. McMillan, Steven W. Knapp, Richard J. Meinersmann
Summary: This study investigated the relationship between ambient weather conditions and the isolation of Campylobacter from chicken flocks. The results showed that seasonality does not affect the rate of Campylobacter isolation from broilers in the warm temperate climate of the Southeastern U.S., but the diversity of isolates was higher in the milder spring and fall seasons.
JOURNAL OF FOOD PROTECTION
(2023)
Article
Veterinary Sciences
Ning Song, Wenlong Zhang, Yue Ding, Dianjun Wu, Zonghao Dai, Li Xu, Yongguo Cao
Summary: Leptospirosis is a global zoonotic disease caused by pathogenic Leptospira, leading to clinical symptoms and even death in infected animals. This study identified a pathogenic strain of Leptospira isolated from a dog, and confirmed its virulence through hamster experiments.
FRONTIERS IN VETERINARY SCIENCE
(2021)