Article
Biotechnology & Applied Microbiology
Rachith Kalgudi, Roya Tamimi, Godfrey Kyazze, Tajalli Keshavarz
Summary: The study revealed that the combined use of farnesol and tyrosol had a better effect on Pseudomonas aeruginosa biofilm growth, especially against the RBHi strain, while the addition of quorum quenchers (QQs) reduced IL-8 secretion by A549 cells.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Microbiology
Wulin Shan, Jinsong Kan, Xueqin Cai, Meiling Yin
Summary: This review provides a comprehensive understanding of the biological characteristics, virulence, and pathogenic mechanisms of mucoid A. baumannii. It highlights the structure, function, and detection methods of mucus and summarizes current studies and unresolved areas related to mucoid A. baumannii.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Immunology
Warren. J. J. Rowe, Deborah. A. A. Lebman, Dennis. E. E. Ohman
Summary: Pseudomonas aeruginosa forms biofilms by producing exopolysaccharides and converts to a mucoid phenotype, which promotes resistance to phagocytic killing. This study reveals that alginate on the bacterial surface inhibits receptor-ligand interactions important for phagocytosis, leading to persistent chronic infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biology
Nurul Najihah Rawi, Mujahidah Mohd Ramzi, Nor Izzati Abd Rahman, Fazilah Ariffin, Jasnizat Saidin, Kesaven Bhubalan, Noor Wini Mazlan, Nor Atikah Mohd Zin, Julius Yong Fu Siong, Kamariah Bakar, Ahmad Khusairi Azemi, Noraznawati Ismail
Summary: This study aimed to determine the antifouling activities of the marine microorganism P. aeruginosa and characterize it as a potential anti-biofouling agent. The bacterial isolate was cultured, and the extract was screened for antibacterial activities. The results showed high inhibition of biofilm formation and reduction in bacteria colonies. The isolated bacteria, identified as Pseudomonas aeruginosa, have the potential to be used as an alternative to toxic antifoulants.
Article
Microbiology
Wulin Shan, Huanhuan Zhang, Jinsong Kan, Meiling Yin, Jiayun Zhang, Lingling Wan, Renliang Chang, Ming Li
Summary: The mucoid phenotype is an important adaptive defense response for Acinetobacter baumannii, impacting its molecular characteristics and virulence. Mucoid strains exhibit multidrug resistance and increased virulence compared to non-mucoid strains. The amount of biofilm production is not correlated with mucoid phenotype or antibiotic resistance in A. baumannii.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Microbiology
Bernhard Peter Kaltschmidt, Ehsan Asghari, Annika Kiel, Julian Cremer, Dario Anselmetti, Christian Kaltschmidt, Barbara Kaltschmidt, Andreas Huetten
Summary: This study investigated the application of magnetron sputtering of various transition metals in antimicrobial coatings, finding that gold had the strongest antibacterial effect. These antimicrobial coatings show great potential for application on household appliances and surgical instruments.
Article
Chemistry, Multidisciplinary
Yiwen Zhu, Shijing Wu, Yujie Sun, Xuan Zou, Liang Zheng, Shun Duan, Julin Wang, Bingran Yu, Ruifang Sui, Fu-Jian Xu
Summary: The newly designed P alpha Gal(50)-b-PGRB(20) can effectively disperse biofilms and selectively kill MDR-P. aeruginosa within biofilms by binding to Lec A in the extracellular polymeric substances and on the P. aeruginosa membrane under low phototoxicity caused by broad-spectrum aPDT. It has bactericidal activities by damaging DNA, RNA, protein, and membrane, showing potential for better corneal recovery in MDR-P. aeruginosa biofilm infected keratitis model.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Engineering, Biomedical
Mojtaba Hedayati Ch, Arefeh Abolhassani Targhi, Farzaneh Shamsi, Fatemeh Heidari, Zahra Salehi Moghadam, Amir Mirzaie, Reyhaneh Behdad, Maryam Moghtaderi, Iman Akbarzadeh
Summary: In this study, niosome-encapsulated tobramycin based on Span 60 and Tween 60 was synthesized and its biological efficacies including anti-bacterial, anti-efflux, and anti-biofilm activities were investigated against multidrug resistant (MDR) clinical strains of Pseudomonas aeruginosa. The results showed that the encapsulated tobramycin exhibited enhanced antibacterial activity and reduced antibiotic resistance in comparison to free tobramycin, making it a promising drug delivery system with low cytotoxicity against HEK293 cells.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Chemistry, Analytical
Luma Clarindo Lopes, Dhesmon Lima, Muhammad Hayat, Yanqi Li, Ayush Kumar, Sabine Kuss
Summary: The emergence and spread of antibiotic resistance is a major challenge to public health. This study presents an electrochemical method for quantifying the retention of tobramycin in susceptible and resistant strains of Pseudomonas aeruginosa, which can distinguish between the two types of strains and identify varying degrees of resistance.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Oliver J. Hills, James Smith, Andrew J. Scott, Deirdre A. Devine, Helen F. Chappell
Summary: The study creates two large mucoid P. aeruginosa EPS models based on beta-D-mannuronate and beta-D-mannuronate-alpha-L-guluronate systems, encompassing thermodynamically stable acetylation configurations unique to mucoid P. aeruginosa. Using first principles calculations, the study identifies stable coordination environments of cations in CF lungs and quantifies their thermodynamic stability. The models demonstrate the weaker cross-linking capability of Na+ and Mg2+ ions compared to Ca2+ ions, with a preference for cation binding within M-G blocks due to smaller torsional rearrangements required to reveal stable binding sites. The geometry of chelation site influences the stability of resulting complexes more than electrostatic interactions, providing nuanced chemical insight into previous experimental observations.
Review
Biotechnology & Applied Microbiology
Anming Xu, Xiaoxiao Zhang, Tong Wang, Fengxue Xin, Luyan Z. Ma, Jie Zhou, Weiliang Dong, Min Jiang
Summary: Pseudomonas aeruginosa is known for its excellent ecological flexibility and can survive in diverse niches. The adaptive mutants RSCVs have been frequently isolated from chronic infections, sparking interest in investigating their formation, persistence, and pathogenesis. RSCVs are believed to play a significant role in biofilm formation and high adaptability.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biochemistry & Molecular Biology
Fabio M. S. Costa, Andreia Granja, Rocio L. Perez, Isiah M. Warner, Salette Reis, Marieta L. C. Passos, M. Lucia M. F. S. Saraiva
Summary: Antimicrobial resistance is a global public health concern, and strategic therapies are needed to replace ineffective antibacterials. GUMBOS, a group of uniform materials based on organic salts, provide a promising alternative approach by combining antibacterials with adjuvants to treat infections caused by resistant bacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Caio H. N. Barros, Dishon W. Hiebner, Stephanie Fulaz, Stefania Vitale, Laura Quinn, Eoin Casey
Summary: Curcumin-functionalised poly(lactic-co-glycolic acid)-dextran micelles are novel nanostructures with intrinsic antibacterial activity, showing enhanced penetration into biofilms and improved antibacterial activity compared to free curcumin. These nanoparticles are also efficient at disrupting mature biofilms and inhibiting bacterial growth within biofilms, suggesting their potential for drug delivery applications.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Chemistry, Medicinal
Maider Ugalde-Arbizu, John Jairo Aguilera-Correa, Eider San Sebastian, Paulina L. L. Paez, Estela Nogales, Jaime Esteban, Santiago Gomez-Ruiz
Summary: Antibiotic resistance is a global problem, and this study aimed to synthesize and characterize seven materials based on silica mesoporous nanoparticles functionalized with fluoroquinolones, Cu2+ or Ag+ species. The antibacterial properties of these materials were evaluated against different strains including Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa. The Ag+ materials exhibited a broader spectrum of antibacterial activity, inhibiting even multi-drug resistant strains, and the material with phenytoin sodium showed potential in reducing biofilm development and inhibiting bacterial growth.
Article
Biochemistry & Molecular Biology
Alicja Chrzanowska, Marta Struga, Piotr Roszkowski, Michal Kolinski, Sebastian Kmiecik, Karolina Jalbrzykowska, Anna Zabost, Joanna Stefanska, Ewa Augustynowicz-Kopec, Malgorzata Wrzosek, Anna Bielenica
Summary: Novel conjugates of moxifloxacin with fatty acids were synthesized and found to have stronger cytotoxic potential than the parent drug. Some of these conjugates showed high cytotoxicity against prostate cancer cells. Additionally, they exhibited activity against standard and clinical bacterial strains, as well as mycobacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Roy Al Ahmar, Brandon D. Kirby, Hongwei D. Yu
JOURNAL OF BACTERIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Meagan E. Valentine, Brandon D. Kirby, Thomas R. Withers, Shannon L. Johnson, Timothy E. Long, Youai Hao, Joseph S. Lam, Richard M. Niles, Hongwei D. Yu
MICROBIAL BIOTECHNOLOGY
(2020)
Article
Microbiology
Brandon D. Kirby, Roy Al Ahmar, T. Ryan Withers, Meagan E. Valentine, Monica Valentovic, Timothy E. Long, James R. Gaskins, Hongwei D. Yu
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2019)
Article
Infectious Diseases
Jonah A. Moore, Michaela Meakin, Mikaela H. Earl, Tiffany M. Kummer, Jeremy P. McAleer, Timothy E. Long
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2020)
Article
Biotechnology & Applied Microbiology
Maria-Paz Cabal, Timothy E. Long, Edward Turos, Ana-Belen Garcia, Jessie L. Allen, Bridget G. Budny, Lindsey N. Shaw
JOURNAL OF ANTIBIOTICS
(2020)
Article
Infectious Diseases
Tyler B. Clay, Kara W. Orwig, Raice A. Stevens, Emily P. Davis, Timothy M. Jennings, Timothy E. Long, Brittany L. Riley, Michael D. Hambuchen
Summary: MRSA PCR wound swabs showed high sensitivity, specificity, and predictive values in SSTIs, demonstrating their potential as a valuable tool.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Yeshi Yin, Miaomiao Li, Weizhong Gu, Benhua Zeng, Wei Liu, Liying Zhu, Xionge Pi, Donald A. Primerano, Hongwei D. Yu, Hong Wei, Guangli Yu, Xin Wang
Summary: The study found that certain bacteria in the human colon can synergistically degrade low molecular weight kappa-carrageenan, leading to enhanced pro-inflammatory effects, which are important markers for evaluating the safety of carrageenans in foods or medicines.
JOURNAL OF GENETICS AND GENOMICS
(2021)
Article
Biotechnology & Applied Microbiology
Alexandria D. Lewis, Taylor M. Riedel, Meredith B. A. Kesler, Melinda E. Varney, Timothy E. Long
Summary: Disulfide analogs of disulfiram showed antimicrobial activity against MRSA and other pathogenic organisms. Lipophilicity and bulkiness of the substituents were found to correlate with the activity, and optimal analogs contained S-octyl disulfides and N,N-dimethyl- or N-pyrrolidine dithiocarbamate substituents.
JOURNAL OF ANTIBIOTICS
(2022)
Article
Infectious Diseases
Claire N. Shanholtzer, Cameron Rice, Katherine Watson, Hannah Carreon, Timothy E. Long
Summary: This study evaluated the impact of copper on the antifungal activity of disulfiram against fluconazole-resistant Candida species. The results showed that copper addition enhanced the inhibitory effects of disulfiram on Candida cultures, but it also antagonized the fungicidal activity of disulfiram.
Article
Biochemistry & Molecular Biology
Adeoluwa A. Adeluola, Nana Bosomtwe, Timothy E. Long, A. R. M. Ruhul Amin
Summary: This study evaluated the anti-tumor efficacy and mechanism of action of ActD in aerodigestive tract cancers. Results showed that ActD strongly inhibited the growth of aerodigestive tract cancer cells and induced efficient apoptosis. Mechanistic studies revealed that ActD increased the expression of p53 and influenced its downstream targets.
Article
Microbiology
Hasitha Chavva, Yogesh Meka, Timothy E. E. Long
Summary: This study found that the combination of disulfiram (DSF) and vancomycin (VAN) can significantly enhance bactericidal activity against methicillin-resistant Staphylococcus aureus (MRSA), but antagonistic effects were observed on the post-antibiotic effect of VAN.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Medicinal
Ebenezer Newton, Sarah A. Starcovic, Michael Menze, Mary E. Konkle, Timothy E. Long, Lori A. Hazlehurst, Jason D. Huber, Aaron R. Robart, Werner J. Geldenhuys
Summary: MitoNEET is a protein belonging to the CDGSH Iron-Sulfur Domain (CISD)-gene family, containing a [2Fe-2S] cluster, and located on the outer membrane of mitochondria. Its specific functions in regulating mitochondrial bioenergetics in metabolic diseases are not fully understood. A protocol for high-throughput screening (HTS) assay targeting mitoNEET has been developed, which facilitates drug discovery for metabolic disorders.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2023)
Review
Biochemistry & Molecular Biology
Lindsay Blume, Timothy E. Long, Edward Turos
Summary: Sulfur-containing molecules have a rich history in bioactivity, particularly as antibacterial agents against infectious pathogens. Natural organosulfur compounds have been used for treating infections since ancient times. Additionally, many commercially available antibiotics also contain sulfur-based components in their structures. In this review, we provide a summary of sulfur-containing antibacterial compounds, with a focus on disulfides, thiosulfinates, and thiosulfonates, and discuss potential opportunities for future advancements in this field.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Kathleen C. Brown, Kushal J. Modi, Reagan S. Light, Ashley J. Cox, Timothy E. Long, Rama S. Gadepalli, John M. Rimoldi, Sarah L. Miles, Gary Rankin, Monica Valentovic, Krista L. Denning, Maria T. Tirona, Paul T. Finch, Joshua A. Hess, Piyali Dasgupta
Summary: Capsaicin, the heterocyclic vanilloid compound, is responsible for the spicy flavor of chili peppers and has been shown to suppress the growth of multiple human cancers. In addition to capsaicin, natural and synthetic capsaicin-like compounds also exhibit growth suppressive activity in human cancers. This manuscript describes the isolation and synthesis of capsaicin analogs with structural modifications in region B, as well as their pharmacokinetic properties, anticancer activity, and signaling pathways underlying their growth-inhibitory effects. The discovery of novel, second-generation region B capsaicin analogs holds promise for innovative nutrition-based combination therapies in human cancers.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Shibin Zhao, Julian Maceren, Mia Chung, Samantha Stone, Raphael Geiben, Melissa L. Boby, Bradley S. Sherborne, Derek S. Tan
Summary: Antibiotic resistance is a major threat to public health, with Gram-negative bacteria presenting unique challenges due to their low permeability and efflux pumps. Limited understanding of the chemical rules for overcoming these barriers hinders antibacterial drug discovery. Efforts to address this issue, such as screening compound libraries and using cheminformatic analysis, have led to the design of sulfamidoadenosines with diverse substituents, showing potential utility in accumulation in Escherichia coli.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Jichun Li, Qing Li, Shuai Xia, Jiahuang Tu, Longbo Zheng, Qian Wang, Shibo Jiang, Chao Wang
Summary: This study successfully developed a short peptide mimetic as a MERS-CoV fusion inhibitor by reproducing the key recognition features of the HR2 helix. The resulting 23-mer lipopeptide showed comparable inhibitory effect to the 36-mer HR2 peptide HR2P-M2. This has important implications for developing short peptide-based antiviral agents to treat MERS-CoV infection.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Krista Jaunsleine, Linda Supe, Jana Spura, Sten van Beek, Anna Sandstrom, Jessica Olsen, Carina Halleskog, Tore Bengtsson, Ilga Mutule, Benjamin Pelcman
Summary: Beta(2)-adrenergic receptor agonists can stimulate glucose uptake by skeletal muscle cells and are therefore potential treatments for type 2 diabetes. The chirality of compounds has a significant impact on the activity of these agonists. This study found that certain synthesized compounds showed higher glucose uptake activity. These findings provide important information for the design of novel beta(2)AR agonists for T2D treatment.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Xin Xu, Jia Chen, Guan Wang, Xiaojuan Zhang, Qiang Li, Xiaobo Zhou, Fengying Guo, Min Li
Summary: The study focuses on EZH2, a promising therapeutic target for various types of cancers. Researchers designed and synthesized a series of novel derivatives aiming to enhance the EZH2 inhibition activity. Among them, compound 28 displayed potent EZH2 inhibition activity and showed high anti-proliferative effects in lymphoma cell lines and xenograft mouse models. The study suggests that compound 28 has potential as a therapeutic candidate for EZH2-associated cancers.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Wei Zhang, Wei Liu, Ya-Dong Zhao, Li-Zi Xing, Ji Xu, Rui-Jun Li, Yun-Xiao Zhang
Summary: This study developed a series of aromatic amide derivatives based on Rhein and investigated their inhibitory activity against alpha-Syn aggregation. Two of these compounds showed promising potential in treating Parkinson's disease by stabilizing alpha-Syn's conformation and disassembling alpha-Syn oligomers and fibrils.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)
Article
Chemistry, Medicinal
Mani Sharma, S. S. S. S. Sudha Ambadipudi, Neeraj Kumar Chouhan, V. Lakshma Nayak, Srihari Pabbaraja, Sai Balaji Andugulapati, Ramakrishna Sistla
Summary: Therapeutically active lipids in drug delivery systems can enhance the safety and efficacy of treatment. The liposome formulation created using synthesized biologically active lipids showed additive anti-cancer effects and reduced tumorigenic potential.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2024)