Article
Cell Biology
Kajohnkiart Janebodin, Rakchanok Chavanachat, Aislinn Hays, Morayma Reyes Gil
Summary: The study aimed to investigate the role of VEGFR-2 in odontoblast differentiation of dental pulp stem cells (DPSCs) by knocking down the expression of VEGFR-2. The results showed that VEGFR-2 is crucial for odontoblast differentiation in DPSCs, and deficiency of VEGFR-2 may impair the differentiation of odontoblast cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Zijie Wang, Chuying Chen, Jiayi Zhang, Jiangdie He, Lin Zhang, Jiayuan Wu, Zhihui Tian
Summary: In this study, it was found that the expression of SCUBE3 protein is transferred from the epithelium to the mesenchyme during embryonic development. In vitro experiments showed that exogenous SCUBE3 promotes the proliferation and migration of human dental pulp stem cells (hDPSCs), and accelerates the differentiation of odontoblasts. Animal experiments demonstrated that SCUBE3 pre-treatment enhances the attachment of odontoblast-like cells and improves angiogenesis performance. These findings provide important insights for the clinical application of SCUBE3 in dental pulp regeneration.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Jiaxin Yin, Jue Xu, Ran Cheng, Meiying Shao, Yuandong Qin, Hui Yang, Tao Hu
Summary: This study found that connexin 43 (Cx43) may be involved in maintaining odontoblast arrangement patterns and influencing pulp repair outcomes by regulating odontoblastic differentiation.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2021)
Article
Dentistry, Oral Surgery & Medicine
J. W. Son, S. H. Choi, J. H. Jang, J. T. Koh, W. M. Oh, Y. C. Hwang, B. N. Lee
Summary: The study found that irisin significantly promoted odontogenic differentiation and angiogenesis in HDPCs, as well as significantly increased cell migration. Phosphorylation of MAPK and Akt was increased by irisin, and inhibitors of these pathways inhibited mineralization and expression of odontogenic and angiogenic markers.
INTERNATIONAL ENDODONTIC JOURNAL
(2021)
Article
Dentistry, Oral Surgery & Medicine
J. Fu, H. Zheng, Y. Xue, R. Jin, G. Yang, Z. Chen, G. Yuan
Summary: WWP2 promotes odontoblastic differentiation by monoubiquitinating KLF5, leading to the regulation of odontoblast marker gene expression.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Yi Zhan, Bing Deng, Huixian Wu, Changpeng Xu, Ruiying Wang, Wenqiang Li, Zhixiong Pan
Summary: Liquid crystals (LCs) are promising biomaterials for bone regenerative medicine due to their tunable physical properties and anisotropic viscoelastic behavior. A novel composite PLLA scaffold manufactured with a simple electrospinning and biomineralization technique precisely controls fibrous structure in liquid LC phase. The enriched-LC composites exhibit superior mineralization ability, and the HAP-PLLA/LC scaffold accelerates bone formation by creating a microenvironment for vascularized regeneration.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Maha R. Taalab, Samia S. Abd El Rehim, Dina W. Eldeeb, Riham M. El-Moslemany, Hams Abdelrahman
Summary: This study evaluated the effect of Aloe vera as an adjunct to Beta-tricalcium phosphate (beta-TCP) bone graft in Grade II furcation defects. The results showed that Aloe vera mixed with beta-TCP resulted in more bone formation and new periodontal ligament (PDL) fibers compared to beta-TCP alone. Therefore, Aloe vera gel adjunctive to beta-TCP is an effective bioactive agent that enhances periodontal tissue regeneration and bone formation in critically sized defects.
SCIENTIFIC REPORTS
(2023)
Article
Cell & Tissue Engineering
Beidi Zhang, Min Xiao, Xiaogang Cheng, Yu Bai, Hanze Chen, Qing Yu, Lihong Qiu
Summary: This study demonstrates that the enamel matrix derivative (EMD) enhances the odontoblastic differentiation of dental pulp stem cells (DPSCs) through triggering MAPK signaling pathways. The findings provide a new insight into the mechanism by which EMD affects DPSCs differentiation and propose EMD as a promising candidate for future stem cell therapy in endodontics.
STEM CELLS INTERNATIONAL
(2022)
Article
Cell & Tissue Engineering
Xinghong Luo, Weiqing Feng, Shijiang Huang, Shenghong Miao, Tao Jiang, Qian Lei, Jingyao Yin, Sheng Zhang, Xiaochun Bai, Chunbo Hao, Weizhong Li, Dandan Ma
Summary: mTORC1 activation inhibits odontoblastic differentiation of dental pulp stem cells by suppressing exosome release from odontoblasts. These findings provide new insights into dental pulp complex regeneration.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell & Tissue Engineering
Liqiang Zhang, Jiangdong Zhao, Jiayi Dong, Yuting Liu, Kun Xuan, Wenjia Liu
Summary: Our study demonstrated that ALPL regulates odontoblastic differentiation of DPSCs and provides valuable information for understanding the mechanisms underlying tooth dysplasia in HPP patients. By modulating the phosphorylation of GSK3 beta, we were able to improve odontoblastic differentiation in HPP DPSCs, and systemic LiCl injection ameliorated tooth-associated defects in ALPL(+/-) mice.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell & Tissue Engineering
Yue Zhang, Hao Zhang, Ziqiu Xiao, Guohua Yuan, Guobin Yang
Summary: In this study, IPO7 was found to promote odontoblastic differentiation and inhibit osteoblastic differentiation by regulating RUNX2 expression and translocation differently.
Article
Biochemistry & Molecular Biology
Xuechun Hu, Wenhao Liu, Lihong Sun, Shilin Xu, Tao Wang, Jie Meng, Tao Wen, Qingqiao Liu, Jian Liu, Haiyan Xu
Summary: In this study, magnetic nanofibrous scaffolds were successfully fabricated and applied for skeletal muscle regeneration. Under the magnetic fields, the scaffolds enhanced myoblast differentiation and VEGF secretion, resulting in accelerated defect repair. Animal experiments also confirmed the effectiveness of the magnetic scaffold in skeletal muscle regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Jinjin Lu, Rui Li, Shilei Ni, Yuandong Xie, Xinchen Liu, Kai Zhang, Yi Li
Summary: The synthesized MCDs in this study achieved efficient odontoblastic differentiation of hDPSCs in vitro, improving the cell differentiation capacity by promoting the expression of odontoblast genes and proteins, and enhancing the process through autophagy mechanism.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Huanyan Zuo, Yao Xiao, Jiahao Han, Yuxiu Lin, Cheng Tian, Shu Zhang, Guohua Yuan, Huan Liu, Zhi Chen
Summary: This study reveals that phosphorylation of ATF2 (p-ATF2) promotes odontoblastic differentiation by remodeling chromatin accessibility. The study also demonstrates the physical interaction between p-ATF2 and H2BK12 acetylation, highlighting the significance of TFs phosphorylation in cell fate transitions.
JOURNAL OF GENETICS AND GENOMICS
(2023)
Article
Polymer Science
Zhou Ye, Yipin Qi, Anqi Zhang, Brandon J. Karels, Conrado Aparicio
Summary: The study developed a method to mineralize collagen with Sr-doped hydroxyapatite (HA) using the polymer-induced liquid precursor (PILP) process. Sr doping altered the crystal lattice of HA and inhibited mineralization in a concentration-dependent manner, but did not affect the unique intrafibrillar mineralization using PILP. The Sr-doped HA nanocrystals aligned in the [001] direction but did not replicate the parallel alignment of pure Ca HA with the collagen fiber long axis. The mimicry of Sr doping in PILP-mineralized collagen has important implications for understanding natural hard tissues and treatment processes.