Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1613, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2019.460692
Keywords
Fish oil; MAGs; DAGs; UHPLC; Bacterial lipase; Nutritional quality
Funding
- Ministiy of Higher Education Scientific research Tunisia
- Programma Operativo Fondo Sociale Europeo (FSE) Regione Siciliana 2014-2020 - Asse 3 Ob. 10.5
- Merck Life Science (Merck KGaA, Darmstadt, Germany)
- Shimadzu Corporation
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The use of an immobilized Serratia. sp W3 lipase as a replacement for the standard pancreatic lipases in the hydrolysis of liver oil from the Musteleus mustelus was studied. Monoacylglycerols (MAGs) and diacylglycerols (DAGs) containing omega-3 polyunsaturated fatty acids, namely eicosapentaenoic and docosahexaenoic acids were produced in hexane solvent at reaction temperatures reaching 55 degrees C with a molar triacylglycerol conversion over than 75 +/- 5% in 24 h showing excellent hydrolysis characteristics. The favorable conditions for the hydrolysis reaction allowed fats with higher melting points to be analyzed facilitating the coupling of the hydrolysis reaction to the later steps in the analytical protocol. The lipid composition was elucidated for the first time by employing a highly efficient UHPLC-MS method with a novel embedded linear retention index approach. MAGs and DAGs obtained during the enzymatic hydrolysis could be used for the production of glycerol based emulsifiers of nutritional interest. (C) 2019 Elsevier B.V. All rights reserved.
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