Journal
JOURNAL OF CHEMICAL EDUCATION
Volume 97, Issue 1, Pages 234-238Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jchemed.9b00593
Keywords
Upper-Division Undergraduate; Analytical Chemistry; Environmental Chemistry; Hands-On Learning/Manipulatives; Collaborative/Cooperative Learning; Water/Water Chemistry; Instrumental Methods
Funding
- Mississippi Water Resources Research Institute
- United States Geological Survey
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Microplastics (MPs) are small plastic particles (<5 mm in size) that are ubiquitous in the environment and have even been detected in bottled water. In this laboratory experiment, suited for instrumental or environmental chemistry classes, students detect and quantify MPs in bottled water by filtering and staining them with Nile red dye prior to utilizing fluorescence microscopy. Instrumental concepts in fluorescence spectroscopy are reinforced as students build a low-cost fluorescence microscope and use it to collect images of the fluorescing MPs for counting purposes and assessing morphology. The exercise introduces students to MP pollution, an emerging field of chemical research, and motivates and engages them helping to form connections beyond the classroom. Each group detected MPs in their bottled water, and many were surprised by how many they found. Overall, the hands-on experiment received positive feedback from students, and postexperiment assessments showed marked improvement in their understanding of the principles of fluorescence and of the growing problem of MP pollution.
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