Article
Medicine, General & Internal
Fatma E. Hassan, Hader I. Sakr, Passant M. Mohie, Howayda Saeed Suliman, Ayman Saber Mohamed, Mohamed H. Attia, Dalia M. Eid
Summary: The study found that PPAR-gamma agonist pioglitazone can alleviate fatigue, improve muscle performance, reduce inflammatory cytokines, and enhance antioxidant activity in a rat model of fibromyalgia. Conversely, the PPAR-gamma antagonist GW9662 worsened the symptoms, indicating a promising role of PPAR-gamma agonists against fibromyalgia-related motor dysfunctions.
ANNALS OF MEDICINE
(2021)
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Peripheral Vascular Disease
Till Schuette, Sarah M. Kedziora, Nadine Haase, Florian Herse, Andreas Busjahn, Anna Birukov, Natalia Alenina, Dominik N. Mueller, Michael Bader, Michael Schupp, Ralf Dechend, Kristin Kraeker, Michaela Golic
Summary: A study conducted on a rat model found that maternal diabetes during pregnancy without obesity did not have a negative impact on the metabolic health of male offspring. There were no significant differences in blood glucose control between offspring exposed to maternal hyperglycemia and those from control pregnancies. Intrauterine exposure to hyperglycemia and hyperinsulinemia without obesity did not lead to fetal programming of diabetes and obesity in this particular model.
Article
Ophthalmology
Satoko Nakano, Yasuhiro Tomaru, Toshiaki Kubota, Hiroshi Takase, Manabu Mochizuki, Norio Shimizu, Sunao Sugita
Summary: The Direct Strip PCR test offers rapid and accurate detection of infectious pathogens causing uveitis. It shows high concordance with qPCR results, even when used by beginners, indicating its reliability for clinical use. Additionally, it can be used for intraoperative diagnosis, improving the prognosis of infectious diseases.
Article
Parasitology
Gereon Schares, Majda Globokar Vrhovec, Mareen Tuschy, Maike Joeres, Andrea Baerwald, Bretislav Koudela, Jitender P. Dubey, Pavlo Maksimov, Franz J. Conraths
Summary: The 529-bp repeat of H. hammondi can be used to establish a quantitative real-time PCR assay, with excellent specificity and sensitivity. This assay is a valuable diagnostic tool for epidemiological and cell biological studies on H. hammondi and related parasites.
PARASITES & VECTORS
(2021)
Article
Optics
Damian Presti, Julian Bergier, Lucas Ripoll, Cristina Borio, Gustavo A. Torchia, Marcos Bilen
Summary: Since the global pandemic of SARS-CoV-2, the need for a low-cost and faster diagnostic tool for evaluating symptomatic patients has emerged. Point-of-care testing and point-of-need testing systems have been developed to provide accurate and rapid diagnostics. In this study, a bio-photonic device using an iso-thermal system was developed for detecting SARS-CoV-2. The device showed comparable sensitivity to the commercially used reference method and was built with simple and low-cost components, making it a high-efficiency and affordable instrument.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2023)
Article
Biochemical Research Methods
Melissa R. Pergande, Vince G. Amoroso, Thu T. A. Nguyen, Wenping Li, Emily Vice, Thomas J. Park, Stephanie M. Cologna
Summary: Naked mole-rats are long-lived animals that exhibit resistance to age-related diseases and possess a unique proteome in their brain regions compared to mice. They show lower levels of fatty acid peroxidation under oxygen-deprived conditions and have enhanced PPAR signaling, suggesting a potential neuroprotective mechanism.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemical Research Methods
Renna L. Nouwairi, Larissa L. Cunha, Rachelle Turiello, Orion Scott, Jeff Hickey, Scott Thomson, Stuart Knowles, Jeff D. Chapman, James P. Landers
Summary: This article describes a field-forward prototype instrument capable of ultra-fast thermal cycling for real-time PCR amplification of DNA and RNA. The instrument can complete 40 cycles of real-time PCR in under 10 minutes, an 84% reduction in time compared to standard assays.
Article
Biochemical Research Methods
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hyejeong Jeong, Ki-Hun Jeong
Summary: A multi-channel plasmonic real-time reverse-transcription polymerase chain reaction system is used to demonstrate high-throughput and multi-target detection of the SARS-CoV-2 virus with four simultaneous reactions in a single assay within 15 min.
Article
Pharmacology & Pharmacy
Dilek Sagir
Summary: The study found that using pioglitazone at different doses during pregnancy can affect the neurons, glial cells, and learning-memory levels in the hippocampus. The high-dose group showed delays in learning and memory, decreased number of pyramidal neurons, and increased density of GFAP-positive cells, while the low-dose group did not show significant differences compared to the control group.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jessika Geisebel Oliveira Neto, Silvia Karl Boechat, Juliana Santos Romao, Lia Rafaella Ballard Kuhnert, Carmen Cabanelas Pazos-Moura, Karen Jesus Oliveira
Summary: Cinnamaldehyde treatment in adolescence has long-term benefits for WAT and BAT metabolism in rat models of obesity, highlighting its potential as a reprogramming nutraceutical in the treatment of childhood obesity.
Article
Chemistry, Analytical
Padideh Mohammadyousef, Miltiadis Paliouras, Mark A. Trifiro, Andrew G. Kirk
Summary: In response to the need for rapid infectious pathogen detection, researchers have been working on adapting PCR for POC applications. This paper introduces a compact plasmonic PCR thermocycler for fast DNA amplification using photothermal heating with gold nanorods and a VCSEL. An ultrasensitive real-time amplicon detection strategy is also reported, based on monitoring UV absorption of the PCR sample cycle-by-cycle. This system allows for high detection sensitivity and does not require specific probes or fluorophores.
Article
Pediatrics
Hyun-Woo Lee, Seung-Beom Han, Jung-Woo Rhim
Summary: This study aimed to determine the subjects for bacterial multiplex polymerase chain reaction (mPCR) testing and to interpret the mPCR test results based on patients' clinical symptoms and diagnoses. The study found that positive mPCR test results were significantly associated with older age, diagnosis of acute gastroenteritis, presence of hematochezia, and absence of cough. Additionally, the identification of invasive pathogens in patients with positive mPCR test results was significantly associated with the diagnosis of acute gastroenteritis, presence of fever and diarrhea, and higher C-reactive protein levels. Therefore, selective bacterial mPCR testing should be performed based on the patients' clinical symptoms and diagnoses.
Article
Chemistry, Multidisciplinary
Shaw-Hwa Parng, Li-An Wu, Chien-Chih Kuo, Ping-Jung Wu, Yu-Yin Tsai, Chih-Hung Lee
Summary: This study proposes a method to generate droplets in a cylindrical tube for emulsion polymerase chain reaction. The validity of a non-planar droplet chip is verified by analyzing the light intensity of liquids in real-time during droplet processing. The study also compares two digital droplet PCR methods for measuring the copy numbers of EGFR.
Article
Biochemistry & Molecular Biology
Dalia M. Abd El Motteleb, Samia Hussein, Mai. M. Hasan, Hala Mosaad
JOURNAL OF CELLULAR BIOCHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Mai M. Hasan, Sally M. Shalaby, Jehan El-Gendy, Eman M. A. Abdelghany
JOURNAL OF CELLULAR BIOCHEMISTRY
(2019)
Article
Obstetrics & Gynecology
Safaa A. Elsalam, Ahmed E. Mansor, Mohamed H. Sarhan, Amany M. Shalaby, Mai A. Gobran, Mohamed A. Alabiad
Summary: This study investigated the expression levels of apoptosis, proliferation, and adhesion-related proteins in trophoblastic tissues of women with recurrent miscarriage, finding higher levels of Bax and P27(kip) proteins in Toxoplasma gondii-positive group and lower levels of Bcl-2, Ki67, E-cadherin, and CD44 proteins. These findings clarify the mechanisms involved in abortion and pregnancy loss.
INTERNATIONAL JOURNAL OF GYNECOLOGICAL PATHOLOGY
(2021)
Article
Infectious Diseases
Samira Metwally Mohammad, Magda Saad Ali, Sara Ahmed Abdel-Rahman, Raghda Abdelrahman Moustafa, Mohamed Hassan Sarhan
Summary: This study aimed to differentiate Cryptosporidium species among children suffering from diarrhea in Sharkyia Governorate, Egypt. It was found that C. hominis genotype was predominant and that significant risk factors associated with Cryptosporidium infection in children were animal contact and residence in rural areas.
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2021)
Article
Parasitology
Samah Hassan Yahia, Samia Elsayed Etewa, Nesreen Saeed Saleh, Samira Metwally Mohammad, Nora Ibrahim Aboulfotouh, Ahmad Mansour Kandil, Mohamed Hassan Sarhan
Summary: This study investigated the effect of chronic toxoplasmosis on the liver and spleen tissues of mice. It found that chronically infected mice exhibited altered histological features and inflammatory reactions in hepatosplenic reticuloendothelial tissues. The liver showed changes in biochemical profile with increased liver enzymes and oxidative stress, while the levels of caspase-3, CD3, and CD138 were markedly expressed in the liver and spleen of infected mice. These findings highlight the persistent impact of latent toxoplasmosis on the host's histological architecture, metabolic, and immunological profile.
JOURNAL OF PARASITOLOGY RESEARCH
(2022)
Article
Parasitology
Samah H. Yahia, Samia E. Etewa, Abd Allah A. Al Hoot, Salwa Z. Arafa, Nesreen S. Saleh, Mohamed H. Sarhan, Suzan I. Rashad, Shimaa S. Hassan
Summary: This study investigated the contamination of raw vegetables, green fodder, and soil samples by parasites in Egypt's East Nile Delta. The results showed the presence of various parasites in the soil and vegetable samples, including Ascaris eggs, Trichuris eggs, and Giardia cysts. The findings highlight the urgent need for strict control measures during the growth period of raw vegetables and green fodder to reduce the transmission of soil-transmitted parasites to humans and animals.
JOURNAL OF PARASITOLOGY RESEARCH
(2023)
Article
Parasitology
M. H. Sarhan, A. Farghaly, N. F. Abd El-Aal, S. Mohammed Farag, A. Ahmed Ali, T. I. Farag
Summary: This study evaluated the efficacy of a combination therapy using selenium nanoparticles and propolis for treating trichinosis. The results showed that this combination reduced the number of adult worms and larvae, decreased inflammation, and inhibited angiogenesis. Therefore, it could be a natural alternative therapy for trichinosis.
JOURNAL OF HELMINTHOLOGY
(2022)
Article
Parasitology
Howayda S. F. Moawad, Samia E. Etewa, Samira M. Mohammad, Mayy A. Neemat-Allah, Jad A. Degheili, Mohamed H. Sarhan
Summary: This study aimed to investigate the seropositivity rate of T. gondii specific antibodies in pediatric hemodialysis patients. The results showed a higher rate of toxoplasmosis in hemodialysis children, and it was associated with contact with cats and eating semi-cooked meat.
PARASITOLOGISTS UNITED JOURNAL
(2022)
Article
Parasitology
Mohamed H. Sarhan, Samia E. Etewa, Abd-Allah A. Al-Hoot, Salwa Z. Arafa, Rodyna A. M. A. Shokir, Howayda S. F. Moawad, Samira M. Mohammad
Summary: The study evaluated the potential effect of mesenchymal stem cells in the treatment of trichinosis, with results showing that combined therapy can significantly reduce the number of intestinal worms and encysted muscular larvae.
PARASITOLOGISTS UNITED JOURNAL
(2021)
Article
Parasitology
Samia E. Etewa, Mohamed H. Sarhan, Howayda S. F. Moawad, Samira M. Mohammad, Mohamed A. Samir, Ahmad M. Kandil, Eman M. Mostafa
Summary: This study investigated the effects of chronic toxoplasmosis on behavioral, histopathological, and immunohistochemical changes in mice infected with T. gondii Me49 strain. The results demonstrated neurobehavioral problems, inflammatory infiltrates, astrocytosis, deteriorated neurons, and meningitis in infected mice compared to controls. Immunohistochemical analysis revealed increased CD3 expression by activated astrocytes, suggesting progressive astrocyte activation over time, while CD138 and caspase-3 expression decreased. Overall, chronic toxoplasmosis led to cognitive and emotional deterioration in infected hosts, resulting in neuropsychiatric and behavioral disturbances.
PARASITOLOGISTS UNITED JOURNAL
(2021)
Article
Parasitology
Samia E. Etewa, Abd-Allah A. Al-Hoot, Hesham M. Share, Howayda S. F. Moawad, Samira M. Mohamed, Mahmoud A. Alshafey, Huda H. Senosy, Mohamed H. Sarhan
PARASITOLOGISTS UNITED JOURNAL
(2019)
Article
Parasitology
Samia E. Etewa, Abd-Allah A. Al-Hoot, Shimaa M. Abdelmoaty, Samira M. Mohammad, Howayda S. F. Moawad, Mohamed H. Sarhan, Sara A. Abdel-Rahman, Mahmoud A. El-Shafey, Eman Z. Abd El-Monem
PARASITOLOGISTS UNITED JOURNAL
(2019)
Article
Reproductive Biology
Mai M. Hasan, Azza A. Abd El Hameed
MIDDLE EAST FERTILITY SOCIETY JOURNAL
(2018)