Journal
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Volume 23, Issue 12, Pages 8381-8391Publisher
WILEY
DOI: 10.1111/jcmm.14718
Keywords
adenomyosis; C; EBP beta; DNA methylation; eutopic endometrium; RNA sequencing
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Funding
- National Natural Science Foundation of China [81771551, 81501276, 81401219]
- Shanghai Municipal Commission of Science and Technology Program [19ZR1462300, 17411972800, 15411966700]
- Shanghai Municipal Commission of Health and Family Planning [20154Y0039]
- Municipal Human Resources Development Program for Outstanding Young Talents in Medical and Health Sciences in Shanghai [2018YQ39]
- Shanghai Shenkang Hospital Development Center Clinical Science and Technology Innovation Project [SHDC12017123]
- Combined Engineering and Medical Project of Shanghai Jiao Tong University [YG2015MS41, YG2017MS39]
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The eutopic endometrium has been suggested to play a crucial role in the pathogenesis of adenomyosis. However, the specific genes in eutopic endometrium responsible for the pathogenesis of adenomyosis still remain to be elucidated. We aim to identify differentially expressed genes (DEGs) and molecular pathways/networks in eutopic endometrium from adenomyosis patients and provide a new insight into disease mechanisms at transcriptome level. RNA sequencing (RNA-Seq) was performed with 12 eutopic endometrium from adenomyosis and control groups. Differentially expressed genes in adenomyosis were validated by quantitative real-time PCR (qPCR) and immunochemistry. Functional annotations of the DEGs were analysed with Ingenuity Pathway Analysis (IPA). Quantitative DNA methylation analysis of CEBPB was performed with MassArray system. A total of 373 differentially expressed genes were identified in the adenomyosis eutopic endometrium compared to matched controls. Bioinformatic analysis predicted that IL-6 signalling and ERK/MAPK signalling were activated in adenomyosis endometrium. We also found that the increased expression and DNA hypomethylation of CEBPB were associated with adenomyosis. Our results revealed key pathways and networks in eutopic endometrium of adenomyosis. The study is the first to propose the association between C/EBP beta and adenomyosis and can improve the understanding of the pathogenesis of adenomyosis.
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