Journal
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
Volume 24, Issue 7, Pages 1087-1103Publisher
SPRINGER
DOI: 10.1007/s00775-019-01719-5
Keywords
Thiazolidine; Oxadiazole; Crystallography; Molecular docking; Antitumor
Funding
- FAPEMIG
- CNPq
- CNPq [409150/2018-5, 304711/2018-7]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES/PROEX) [001]
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Synthesis of dinuclear oxadiazole-adamantane platinum(II) and palladium(II) complexes (PtO, PdO) and mononuclear thiazolidine derivative complexes (PtT, PdT) was described. Characterization was performed by elemental analysis, infrared, UV-visible, H-1, C-13, Pt-195 NMR spectra, MS spectroscopy and single crystal X-ray diffraction. The cytotoxicity by MTT assay against tumor and normal cell lines with or without extracellular GSH was also investigated. In general, mononuclear complexes containing thiazolidine-adamantane ligands were more cytotoxic than oxadiazole-adamantane derivatives. PtT complex proved to be as active as cisplatin. Dinuclear compounds were considered inactive to cells in evaluated conditions, due to their high stability with ligands in a chelated and bridged way. Results suggest that GSH cannot be considered a target. DNA- and BSA-binding interactions were evaluated using UV-visible and fluorescence spectroscopy, intercalating dyes and molecular docking. Upon coordination to platinum(II), the cytotoxic effect was appreciably improved against tested cell lines, in comparison to free thiazolidine ligand. Comparing thiazolidine derivatives, it is noticeable that the less active compound (PdT) presents stronger interaction with BSA, while PtT has the weaker interaction with BSA and relatively strong binding to isolated DNA, resulting in the most cytotoxic complex. This work shows that the presence of metal is significant but it should be available for interaction. The high lability of palladium complex made this stay retainable in BSA and two metal atoms do not increase activity if it is not able to do any interaction. [GRAPHICS]
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