Article
Polymer Science
Banafsheh Safari, Marziyeh Aghazadeh, Leila Roshangar, Ayuob Aghanejad, Soodabeh Davaran
Summary: This study reports the fabrication and evaluation of a bioactive hybrid scaffold collagen/P-PCL for bone tissue engineering. The scaffold showed biocompatibility, osteoconductivity, and osteoinductivity, supporting the proliferation and differentiation of AD-MSCs.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Engineering, Biomedical
R. Tevlin, H. desJardins-Park, J. Huber, S. E. DiIorio, M. T. Longaker, D. C. Wan
Summary: Osteoarthritis is a global burden that affects joints and bones. Tissue engineering, specifically adipose-derived stromal cells, shows promise as a treatment option. Advancements in material science allow for improved engraftment of these cells and activation of biophysical signals through designed microstructures.
Review
Materials Science, Biomaterials
Yiping Song, Ning Wang, Huixin Shi, Dan Zhang, Qiang Wang, Shu Guo, Shude Yang, Jia Ma
Summary: Bone tissue engineering, supported by scaffold, seed cells, and bioactive molecules, offers new hope for treating bone defects. Adipose-derived stem cells (ADSCs) are favored by researchers due to their abundant sources and multi-differentiation potentials. However, there is a lack of systematic theoretical basis for selecting suitable biomaterials loaded with ADSCs.
REGENERATIVE BIOMATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Gen Li, Yin Zhang, Jiezhou Wu, Renhao Yang, Qi Sun, Yidong Xu, Bo Wang, Ming Cai, Yang Xu, Chengyu Zhuang, Lei Wang
Summary: This study explores the effects and mechanism of adipose stem cells-derived exosomes (ADSCs-Exos) and ADSCs-Exos modified tissue engineering scaffold on bone defect repair. The results demonstrate that ADSCs-Exos promote the proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and ADSCs-Exos modified scaffold enhances the repair of BMSCs and bone defects both in vitro and in vivo.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Ashley L. Farris, Dennis Lambrechts, Yuxiao Zhou, Nicholas Y. Zhang, Naboneeta Sarkar, Megan C. Moorer, Alexandra N. Rindone, Ethan L. Nyberg, Alexander Perdomo-Pantoja, S. J. Burris, Kendall Free, Timothy F. Witham, Ryan C. Riddle, Warren L. Grayson
Summary: Oxygen delivery plays a crucial role in enhancing the therapeutic efficacy of cell transplants. In this study, researchers developed a novel scaffold material that effectively releases oxygen, leading to improved bone tissue regeneration mediated by adipose-derived stem cells.
Article
Engineering, Biomedical
Yong Cheol Shin, Ji-Hyeon Bae, Jong Ho Lee, Iruthayapandi Selestin Raja, Moon Sung Kang, Bongju Kim, Suck Won Hong, Jung-Bo Huh, Dong-Wook Han
Summary: In this study, a bioactive surface modification for implant interface was implemented using reduced graphene oxide-coated titanium. The results showed that the graphene-coated titanium had better cell attachment and proliferation, alkaline phosphatase activity, matrix mineralization, expression of osteogenesis-related genes and protein, and osseointegration compared to the control group. This suggests that graphene-coated titanium could be a promising material for dental or orthopedic implants.
BIOMATERIALS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Aida Selaru, Alexandra-Elena Mocanu-Dobranici, Elena Olaret, Raluca-Elena Ginghina, Izabela-Cristina Stancu, Marieta Costache, Sorina Dinescu
Summary: In this study, fibrous nanostructured substrates based on fish gelatin enriched with graphene oxide and magnetic nanoparticles were fabricated. The biological properties of these substrates, including cell viability, adhesion, and differentiation, were evaluated. The results showed good interaction between the materials and cells, indicating their potential for tissue engineering applications, with a concentration of 0.5% graphene oxide and magnetic nanoparticles being the most suitable option for biological testing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Stefano Negri, Yiyun Wang, Takashi Sono, Qizhi Qin, Ginny Ching-Yun Hsu, Masnsen Cherief, Jiajia Xu, Seungyong Lee, Robert J. Tower, Victoria Yu, Abhi Piplani, Carolyn A. Meyers, Kristen Broderick, Min Lee, Aaron W. James
Summary: Research has shown that anti-DKK1 neutralizing antibodies can enhance the osteogenic differentiation of human adipose-derived stem cells, aiding in bone repair, and demonstrating potential beneficial effects in a mouse model.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Engineering, Biomedical
Moon Sung Kang, Seung Jo Jeong, Seok Hyun Lee, Bongju Kim, Suck Won Hong, Jong Ho Lee, Dong-Wook Han
Summary: In this study, rGO was uniformly coated on Ti substrates using the MDD process, resulting in decreased surface roughness and contact angle. The rGO-Ti substrates significantly enhanced cell proliferation, promoted ALP activity, and matrix mineralization, indicating superior bioactivity and osteogenic potential for dental and orthopedic applications.
BIOMATERIALS RESEARCH
(2021)
Article
Cell & Tissue Engineering
Jana C. Blum, Thilo L. Schenck, Alexandra Birt, Riccardo E. Giunta, Paul S. Wiggenhauser
Summary: In this study, the combination of cell-derived artificial extracellular matrix with 3D printed polycaprolactone scaffolds showed promising results in improving bioactivity and differentiation of human adipose derived stem cells. The ac/aoPCL scaffolds demonstrated good biocompatibility and enhanced chondrogenic and osteogenic differentiation potential.
JOURNAL OF TISSUE ENGINEERING
(2021)
Review
Cell Biology
Kangkang Zha, Yue Tian, Adriana C. Panayi, Bobin Mi, Guohui Liu
Summary: Bone tissue engineering offers a new approach to treating bone defects, with mesenchymal stem cells being a promising seed cell due to their functions in osteogenic differentiation. Improving methods of origin selection, culture conditions, and biophysical stimulation can enhance MSC osteogenesis and contribute to the development of MSC-based bone tissue engineering.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Physical
Miriam Filippi, Boris Dasen, Arnaud Scherberich
Summary: The magneto-sonoporation (MSP) technique allows for instant labeling of transplantable cells with iron oxide nanoparticles through permeabilizing the cell membrane with ultrasound. When applied to adipose-derived stem cells (ASCs), MSP altered the metabolic activity of the cells and induced differentiation towards osteoblastic profile. This novel role of MSP in remote biophysical stimulation of cells could be beneficial for bone tissue repair.
Review
Engineering, Biomedical
Ziyi Feng, Meiqi Jin, Junzhi Liang, Junning Kang, Huazhe Yang, Shu Guo, Xiaoting Sun
Summary: Bone damage may require bone grafting and bone tissue engineering can be an alternative strategy. Mesenchymal stem cells (MSCs) are important for tissue engineering due to their ability to differentiate into various cell types. The regulation of MSCs differentiation and the role of mitochondria in this process are crucial for efficient bone regeneration.
ACTA BIOMATERIALIA
(2023)
Review
Biochemistry & Molecular Biology
Zhaozhao Ding, Weinan Cheng, Md Shipan Mia, Qiang Lu
Summary: Silk, a natural fibrous polymer, shows potential in regenerative medicine and bone tissue engineering. It is processed into scaffolds, hydrogels, and films to facilitate bone regeneration. Bioactive factors and stem cells are introduced to create osteogenic microenvironments for directing bone regeneration.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Mirela Violeta Serban, Simona-Rebeca Nazarie (Ignat), Sorina Dinescu, Ionut-Cristian Radu, Catalin Zaharia, Elena-Alexandra Istratoiu, Eugenia Tanasa, Hildegard Herman, Sami Gharbia, Cornel Balta, Anca Hermenean, Marieta Costache
Summary: Three-dimensional hydrogels and nanostructured clay particles have shown great potential for tissue engineering. In this study, hydrogels enriched with sericin and fibroin were prepared using modified montmorillonite clay as a crosslinking agent. The biocompatibility and tissue regeneration support of the hydrogels were evaluated both in vitro and in vivo, and the hydrogel composed of 95% HEMA and 5% AMPSA enriched with sericin and fibroin showed the best results.
Article
Agronomy
Tatiana Dumitra Panaite, Petru Alexandru Vlaicu, Mihaela Saracila, Ana Cismileanu, Iulia Varzaru, Sorina Nicoleta Voicu, Anca Hermenean
Summary: This study evaluated the effects of dietary inclusion of watermelon rind and sea buckthorn meal on the performance, blood parameters, gut microbiota, and gut morphology of laying hens. The results showed that these dietary ingredients can have a beneficial impact on the biochemical parameters, gut microbiota, and gut morphology of hens.
Article
Biochemistry & Molecular Biology
Aida Selaru, Alexandra-Elena Mocanu-Dobranici, Elena Olaret, Raluca-Elena Ginghina, Izabela-Cristina Stancu, Marieta Costache, Sorina Dinescu
Summary: In this study, fibrous nanostructured substrates based on fish gelatin enriched with graphene oxide and magnetic nanoparticles were fabricated. The biological properties of these substrates, including cell viability, adhesion, and differentiation, were evaluated. The results showed good interaction between the materials and cells, indicating their potential for tissue engineering applications, with a concentration of 0.5% graphene oxide and magnetic nanoparticles being the most suitable option for biological testing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Alexandra-Elena Mocanu-Dobranici, Marieta Costache, Sorina Dinescu
Summary: Magnetic materials and stimulation have been widely studied in tissue engineering, especially in the reconstruction of bone and nervous tissue. Magnetic fields and nanoparticles can modulate cell responses and influence cellular structures and gene expression through complex mechanisms. The use of magnetic stimuli can promote stem cell evolution and neurogenic differentiation, as well as regulate calcium channels and CREB phosphorylation in neurodifferentiation. This review provides an updated perspective on the molecular mechanisms behind the cell response to magnetic cues and their potential applications in nervous tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Liliana-Roxana Balahura Stamat, Sorina Dinescu, Marieta Costache
Summary: Researchers have studied the molecular mechanisms of breast cancer initiation and progression, particularly TNBC, to find specific biomarkers for innovative therapeutic strategies. TNBC is characterized by the absence of certain receptors, making it dynamic and aggressive. TNBC progression is associated with dysregulation of the NLRP3 inflammasome, leading to the release of pro-inflammatory cytokines and caspase-1 dependent cell death. Non-coding RNAs play a significant role in the assembly of the NLRP3 inflammasome, TNBC progression, and metastasis, offering potential for diagnosis and therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Caterina Claudia Lepre, Marina Russo, Maria Consiglia Trotta, Francesco Petrillo, Fabiana Anna D'Agostino, Gennaro Gaudino, Giovanbattista D'Amico, Maria Rosaria Campitiello, Erminia Crisci, Maddalena Nicoletti, Carlo Gesualdo, Francesca Simonelli, Michele D'Amico, Anca Hermenean, Settimio Rossi
Summary: Diabetic retinopathy (DR) is a common microvascular retinal complication, leading to vision loss in diabetic patients. This review focuses on the molecular basis of retinal neuroinflammation, which has been identified as a key factor in the progression of DR. The review discusses the exacerbation of endoplasmic reticulum stress, activation of the NLRP3 inflammasome, role of galectins, and activation of the P2X7 receptor. The selective inhibition of galectins and the P2X7 receptor is proposed as a potential pharmacological approach to prevent DR progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Andreea D. Lazar, Sorina Dinescu, Lea Sleiman, Adrian V. V. Dumitru, Mariana Costache, Marieta Costache
Summary: Cutaneous melanoma is a highly aggressive and often fatal cancer in its metastatic stages. Limited treatment options are available, and its global incidence is rapidly increasing. By comparing gene and small non-coding RNA expression profiles from different stages of melanoma progression, we have identified abnormal regulation of molecular markers involved in processes such as extracellular matrix remodeling, migration, and angiogenesis. Our findings suggest potential targets for microRNAs and have implications for targeted therapy and clinical management.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Maria Consiglia Trotta, Hildegard Herman, Cornel Balta, Marcel Rosu, Alina Ciceu, Bianca Mladin, Carlo Gesualdo, Caterina Claudia Lepre, Marina Russo, Francesco Petrillo, Gorizio Pieretti, Francesca Simonelli, Settimio Rossi, Michele D'Amico, Anca Hermenean
Summary: Repeated oral vitamin D3 supplementation can prevent corneal epithelium damage in an SS mouse model. Mice receiving vitamin D3 supplementation showed preserved corneal epithelium morphology and reduced levels of TACE, neovascularization, and fibrosis markers.
Article
Green & Sustainable Science & Technology
Lisa Sougrati, Sophie Wendels, Sorina Dinescu, Liliana-Roxana Balahura (Stamat), Lea Sleiman, Luc Averous
Summary: In this study, sustainable macromolecular architectures were designed and tested to synthesize fully biobased polyfunctional NCO terminated prepolymers. Two-component adhesive systems were prepared, and the properties of the resulting biobased polyurethanes were studied, showing good biocompatibility and potential biomedical application.
SUSTAINABLE MATERIALS AND TECHNOLOGIES
(2023)
Article
Biochemistry & Molecular Biology
Roxana-Olimpia Gheorghe, Andreea Violeta Grosu, Melania Magercu, Mihail-Sebastian Ghenghea, Cristina Elena Zbarcea, Alexandra Tanase, Simona Negres, Alexandru Filippi, Gabriela Chiritoiu, Mihaela Gherghiceanu, Sorina Dinescu, Gisela Gaina, Damir Sapunar, Violeta Ristoiu
Summary: In the early stages after traumatic nerve injury, resident macrophages in the dorsal root ganglia play a crucial role in the development of neuropathic pain. By interacting with the cytoskeleton, these macrophages can be transformed into an anti-inflammatory phenotype, leading to a reduction in neuropathic pain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
Andreea M. Pandele, Aida Selaru, Sorina Dinescu, Marieta Costache, Eugeniu Vasile, Constanta Dascalu, Matei D. Raicopol, Mircea Teodorescu
Summary: This study aims to obtain porous scaffolds with improved mechanical properties and biocompatibility by using polymer-modified graphene oxide (GO@PPF) in poly(propylene fumarate)/N-vinyl pyrrolidone(PPF/NVP) networks. The GO@PPF nanofiller was synthesized through a facile and convenient surface esterification reaction, and its successful functionalization was demonstrated. The incorporation of GO@PPF in the polymer matrix leads to denser and more homogenous networks, enhancing the mechanical properties, and the mineralized PPF/NVP/GO@PPF scaffolds showed good biocompatibility with murine pre-osteoblasts in terms of cell viability and proliferation.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
