Preparation and characterization of semi-IPNs of polycaprolactone/poly (acrylic acid)/cellulosic nanowhisker as artificial articular cartilage

Cartilage is a semi-solid resilient and smooth elastic connective tissue and upon damage, its repair is almost impossible or occurs with a very slow recovery process. Polycaprolactone (PCL), used as a biocompatible polymer, withholds all required mechanical properties, except suitable cell adhesion due to its hydrophobicity. In order to resolve this issue, we sought to introduce appropriate semi-IPNs into the system to regain its hydrophilicity base on increasing of the hydrophilic polymer. PCL and Cellulose nanowhiskers (CNWs) were entrapped in a network of poly (acrylic acid) that had been crosslinked via a novel acrylic-urethane crosslinker. The influential synthetic parameters on the preparation of artificial articular cartilages were investigated based on the Taguchi test design. The prepared CNW, acrylicurethane crosslinker and semi-IPNs were studied via H-1 NMR, FTIR, SEM, TEM, TGA, water swelling, water contact angle, tensile, and MTT analyses. According to the results, the optimal amount of monomer was about 46%. Incorporation of an optimized amount of CNW, which was 0.5%, improved the mechanical properties of artificial cartilage. After a 30 h time period, semi-IPNs showed the water absorption of about 30%. MIT on days 1, 3 and 5, as well as cell attachment, confirmed the biocompatibility of the semi-IPNs. (C) 2019 Elsevier B.V. All rights reserved.