Article
Food Science & Technology
Atiyeh Heidary, Nafiseh Soltanizadeh
Summary: The aim of this study was to investigate the effects of high-pressure homogenization (HPH) on the functional, physicochemical, and structural properties of gelatin. The results showed that HPH could destroy hydrogen bonds and lead to protein unfolding. The microstructure of gelatin gel was altered, and the emulsifying and foaming properties of gelatin were improved after HPH treatment.
FOOD AND BIOPROCESS TECHNOLOGY
(2023)
Article
Food Science & Technology
Zhaofang Liu, Zixuan Guo, Di Wu, Xu Fei, Hesham R. Ei-Seedi, Cong Wang
Summary: High-pressure homogenization (HPH) was found to significantly impact the structure and functional properties of oyster protein (CGP), leading to improved solubility and water holding capacity, while showing varying effects on emulsion activity and foaming ability at different pressure levels.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Xiaohua Nie, Haizhen Liu, Ningxiang Yu, Qiwen Tang, Congcong Wu, Xianghe Meng
Summary: High pressure homogenization treatment can improve the solubility, emulsifying and foaming properties of fish myofibrillar protein in low salt media, disrupt the aggregates into smaller and more uniform size, and unfold the structure into a more flexible state.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Food Science & Technology
Meishan Wu, Xiaoye He, Duo Feng, Hu Li, Di Han, Qingye Li, Boya Zhao, Na Li, Tianxin Liu, Jing Wang
Summary: It has been demonstrated that high-pressure homogenization (HPH) can enhance the functional properties of proteins. This study investigated the effects of HPH on the structural and functional properties of whey-soy dual-protein (SPI-WPI). The results showed that HPH reduced the particle size of SPI-WPI, altered its secondary and tertiary structures, and improved the hydrophobic interaction between molecules. HPH also improved the solubility and emulsification of all proteins, with the greatest improvement observed in SPI-WPI. Additionally, HPH at 60 MPa significantly enhanced the emulsion properties of SPI-WPI, including reducing droplet size and improving elastic properties and physical stability. This study provides a theoretical basis for the application of HPH in modifying the structure of dual-protein to enhance its development and utilization in liquid specialty food.
Article
Biochemistry & Molecular Biology
Tugba Baskinci, Osman Gul
Summary: The effect of high pressure homogenization (HPH) on the microstructural, techno-functional and rheological properties of sesame protein isolate (SPI) was investigated in this study. The results showed that HPH treatment caused partial changes in the secondary structure of SPI and had a significant effect on the tertiary structure. The best techno-functional and rheological properties were achieved at 100 MPa pressure. HPH modification also led to a decrease in the gelation temperature of SPI by about 15 degrees C.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Min Hyeock Lee, Hae In Yong, Yun Jeong Kim, Yun-Sang Choi
Summary: In this study, the effects of high-pressure homogenization (HPH) on the physicochemical, rheological, and emulsifying properties of myofibrillar proteins (MFPs) were investigated. The results showed that HPH treatment decreased the particle size of MFPs and resulted in more uniform and homogeneous protein particles. HPH treatment also caused fragmentation of high molecular weight proteins and increased the surface hydrophobicity of MFPs. Additionally, HPH treatment improved the emulsifying activity and stability of MFPs.
Article
Chemistry, Physical
Baohong Zeng, Kajsa Nilsson, Paulo Gonsalves Teixeira, Bjorn Bergenstahl
Summary: The trend of using protein produced from microorganisms as a meat substitute is growing popular due to its potential to produce meat-like food products and other functional properties. Effective use of mycoprotein for food applications requires the degradation of cell walls, and the research on filamentous fungal protein functionalities offers an opportunity to increase the potential usage of filamentous fungal biomass in food applications. High pressure homogenization was found to be the most effective method for cell wall degradation, and a pH-shifting procedure was performed for protein recovery.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Food Science & Technology
Zehang Guo, Xingzhou Li, Daichenling Yang, Ailing Lei, Fusheng Zhang
Summary: High-pressure homogenization (HPH) can improve the functional and structural characteristics of chestnut starch. By increasing pressure, cycles, and concentration, the solubility, swelling power, and transparency of starch can be enhanced, while its crystallinity and particle size may decrease.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Food Science & Technology
Huipeng Liu, Yiyuan Xu, Shuyu Zu, Xuee Wu, Aimin Shi, Jinchuang Zhang, Qiang Wang, Ning He
Summary: This review provides a comprehensive summary of the changes in the conformational structure and gel properties of myofibrillar protein (MP) under high hydrostatic pressure (HHP), and discusses the relationship between the spatial structure of MP and meat texture under HHP. The review also explores considerations regarding the effectiveness of HHP as a strategy in future meat manufacturing.
Article
Food Science & Technology
Yiqing Zhu, Xinyang Sun, Jian Ding, Fengjiao Fan, Peng Li, Ji Xia, Xieqi Luo, Yong Fang
Summary: The study successfully prepared a novel NPs material through the electrostatic self-assembly of xanthan gum and lysozyme using HPH technology. The HPH treatment resulted in smaller particle size, larger surface charge, circular shape, and compact surface structure of the HPH-NPs. Changes in the secondary and tertiary structures of lysozyme, increased hydrophobicity, and potential factors causing the formation of HPH-NPs were observed. The HPH-NPs exhibited good solubility, emulsification, and antimicrobial activity, showing potential as a beverage ingredient for inhibiting pathogen growth and ensuring product safety.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Acoustics
Xiao-hong Deng, Xiang-xiang Ni, Jia-hui Han, Wen-hua Yao, Ya-jie Fang, Qin Zhu, Ming-feng Xu
Summary: This study investigated the effects of high-intensity ultrasound treatment on the functional properties and emulsion stability of Neosalanx taihuensis myofibrillar protein (MP). The results showed that ultrasound treatment increased the carbonyl groups, emulsification properties, intrinsic fluorescence intensity, and surface hydrophobicity of the MP solution. The ultrasound treatment also caused changes in the secondary structure of MP, reduced the content of total sulfhydryl, and led to MP cross-linking. Furthermore, ultrasound decreased the size of emulsion droplets and improved the oxidative stability of N. taihuensis MP emulsions.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Nutrition & Dietetics
Yaru Liang, Yanan Guo, Yuxuan Zheng, Sibo Liu, Tianfu Cheng, Linyi Zhou, Zengwang Guo
Summary: This study investigated the combined modification of soybean protein concentrate (SPC) using Alcalase protease and high-pressure homogenization (HPH). The results showed that SPC exhibited optimal physicochemical and functional properties under a homogenization pressure of 60 MPa, with characteristics such as the lowest beta-sheet content, highest water and oil holding capacities, and best emulsifying activity and stability.
FRONTIERS IN NUTRITION
(2022)
Article
Food Science & Technology
Mengting Chen, Lan Wang, Bijun Xie, Aimin Ma, Kai Hu, Changliang Zheng, Guangquan Xiong, Liu Shi, Anzi Ding, Xin Li, Yu Qiao, Zhida Sun, Wenjin Wu
Summary: This study investigated the effects of four treatments on the structure, hydrolysis property, and antioxidant activity of bighead carp myofibrillar protein. The results showed that all treatments increased the hydrolysis and antioxidant capacity of the protein. Among them, the U-H treatment exhibited the highest performance and could be a promising method for preparing bioactive peptides.
FOOD AND BIOPROCESS TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chang Su, Zhifei He, Zefu Wang, Dong Zhang, Hongjun Li
Summary: High pressure homogenization can increase the solubility of myofibrillar proteins, but excessive cycles may lead to protein re-aggregation. Myosin forms soluble polymers of different molecular weights through disulfide bonds during the process.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Chunmin Guan, Ying Wu, Lijuan Luo, Fan Qiao, Jianhua Zhu, Qinlu Lin, Chun Liu
Summary: This study prepared curcumin@high-pressure homogenization-soybean 7S protein/nanoparticles (CUR@HPH-7S-NPs) using an anti-solvent method. The results showed that CUR@HPH-7S-NPs had better size, encapsulation efficiency, and zeta-potential values compared to untreated 7S protein and CUR. The study also found that HPH-7S had better physical thermal stability and improved bio-accessibility of CUR.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)