Article
Agriculture, Dairy & Animal Science
Artur Bryja, Grzegorz Latosinski, Maurycy Jankowski, Ana Angelova Volponi, Paul Mozdziak, Jamil A. Shibli, Rut Bryl, Julia Spaczynska, Hanna Piotrowska-Kempisty, Krzysztof Krawiec, Bartosz Kempisty, Marta Dyszkiewicz-Konwinska
Summary: Domestic pigs have high phylogenetic similarity to humans and are used in biomedical research. Porcine tissues are used in human skin transplants and tissue reconstruction. Transcriptional analysis and live-cell imaging are powerful tools to study complex biological systems, such as porcine oral mucosal cells. Transcriptional analysis revealed gene expression changes over time in in vitro culture, while computer analysis of images allowed identification of different cell populations.
Article
Cell & Tissue Engineering
Selina Wrublewsky, Andrea Weinzierl, Isabelle Hornung, Leticia Prates-Roma, Michael D. Menger, Matthias W. Laschke, Emmanuel Ampofo
Summary: The co-transplantation of pancreatic islets and microvascular fragments from adipose tissue is a promising strategy to improve the success rates of islet transplantation, as it accelerates the restoration of normoglycemia and increases the number of islet cells and microvessels in the grafts.
NPJ REGENERATIVE MEDICINE
(2022)
Article
Biotechnology & Applied Microbiology
Anja E. Luengen, Maria Cheremkhina, Julian Gonzalez-Rubio, Jan Weckauf, Caroline Kniebs, Hendrik Uebner, E. Miriam Buhl, Christian Taube, Christian G. Cornelissen, Thomas Schmitz-Rode, Stefan Jockenhoevel, Anja Lena Thiebes
Summary: The study shows that tri-cultures supported by BM-MSC and HNF are most similar to native respiratory epithelium in terms of epithelial morphology, ciliation, and mucin production. They also promote mucociliary differentiation and vascularization. Ciliation is reduced in tri-cultures compared to co-cultures, while vascular networks are more branched and extended. Cytokine concentrations in tri-cultures are lower than in co-cultures.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Katharina Peters, Nadine Wiesmann, Diana Heimes, Roxana Schwab, Peer W. Kammerer, Bilal Al-Nawas, Ronald E. Unger, Annette Hasenburg, Walburgis Brenner
Summary: Oral mucosa equivalents can be used as grafts for tissue reconstruction. Extracorporeal shock wave therapy (ESWT) has been shown to stimulate the formation and differentiation of epithelium in vitro, leading to a more distinct and differentiated multilayered epithelium and potentially improving the function of tissue equivalents.
Article
Biochemistry & Molecular Biology
Hyeonyu Kim, Tatsuya Osaki, Roger D. Kamm, H. Harry Asada
Summary: This study develops an engineered muscle tissue with a coaxial three-layered tubular structure that resembles the architecture of native skeletal muscles. By remodeling the extracellular matrix, the engineered skeletal muscle tissues produced stronger contractile force and improved angiogenesis.
Article
Biochemistry & Molecular Biology
Tian Hou, Miaomiao Du, Xiang Gao, Meiwen An
Summary: The treatment of skin defects is a challenge in the medical field. Tissue engineering skin provides a new idea for the treatment of injured skin. However, slow vascularization is a problem in tissue engineering due to single seed cells. Cell co-culture technology is studied to simulate the survival and communication environment of cells in the human body. This paper investigates the effects of co-culturing human skin fibroblasts (HSFs) and human vascular endothelial cells (HVECs) on cell activity, migration, and secretion factors. The results show that HVECs can promote cell activity, migration, and secretion factors, improving the success rate of tissue engineering skin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Dentistry, Oral Surgery & Medicine
Koya Nakamura, Toshiro Yamamoto, Ryo Ema, Kei Nakai, Yoshiki Sato, Kenta Yamamoto, Keiji Adachi, Fumishige Oseko, Yoshiaki Yamamoto, Narisato Kanamura
Summary: The study found that mechanical stress applied through dentures increases the production of inflammatory cytokines from oral mucosal epithelial cells and fibroblasts, likely through the p38 MAPK cascade, leading to inflammation. On the other hand, up-regulation of growth factors may be a biological defense mechanism against excessive mechanical stress.
Review
Oncology
Ghazaleh Hashemi, James Dight, Kiarash Khosrotehrani, Laura Sormani
Summary: Melanoma is an aggressive and potentially lethal form of skin cancer. The role of blood vessel formation, particularly endothelial progenitor cells (EPCs), in melanoma is of significance. Understanding the role of EPCs is important for understanding the abnormal vessel structures in melanoma. The effect of anti-angiogenic drugs on melanoma treatment needs further research.
Article
Peripheral Vascular Disease
Vivien Becker, Xin Hui, Lisa Nalbach, Emmanuel Ampofo, Peter Lipp, Michael D. Menger, Matthias W. Laschke, Yuan Gu
Summary: The study demonstrated that linalool can effectively inhibit the angiogenic activity of endothelial cells, which may be mediated by regulating the ERK signaling pathway, downregulating intracellular ATP levels, and activating TRPM8 channels.
Article
Biochemistry & Molecular Biology
Helen E. Driessen, Stephanie M. van der Voorn, Mimount Bourfiss, Freyja H. M. van Lint, Ferogh Mirzad, Laila El Onsri, Marc A. Vos, Toon A. B. van Veen
Summary: In arrhythmogenic cardiomyopathy, pathogenic variants are found in genes encoding desmosomal proteins and non-desmosomal genes. Plakoglobin protein levels and localization are disturbed in ACM patients and PLN p.Arg14del patients. BMC can serve as a non-invasive tool with promising potential for ACM diagnosis, as plakoglobin levels are significantly reduced in ACM patients. However, interindividual variability makes it difficult to distinguish true patients from carriers and controls.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Se Eun Ha, Byungchang Jin, Brian G. Jorgensen, Hannah Zogg, Lai Wei, Rajan Singh, Chanjae Park, Masaaki Kurahashi, Sei Kim, Gain Baek, Sandra M. Poudrier, Moon Young Lee, Kenton M. Sanders, Seungil Ro
Summary: Researchers isolated MuPαC cells from colonic mucosal tissue, obtained their transcriptome data, identified selectively expressed markers and enriched genetic signatures, and added the data to an online resource for comprehensive reference of genetic transcripts of MuPαC and related cells in the mouse colon and jejunum.
Article
Multidisciplinary Sciences
Marina Sanaki-Matsumiya, Mitsuhiro Matsuda, Nicola Gritti, Fumio Nakaki, James Sharpe, Vikas Trivedi, Miki Ebisuya
Summary: This study reports the generation of human somitoids, organoids that periodically form epithelial somite-like structures. Somitoids display oscillations of the segmentation clock and have proper polarity.
NATURE COMMUNICATIONS
(2022)
Article
Endocrinology & Metabolism
Haitao Zhang, Yan Shen, Il-man Kim, Neal L. Weintraub, Yaoliang Tang
Summary: Diabetes leads to abnormal cellular metabolism in CMECs, affecting glycolysis and mitochondrial oxidative phosphorylation, resulting in increased intracellular ROS levels. Gene expression and protein levels differ between diabetic and WT CMECs. Additionally, diabetic CMECs exhibit decreased cell proliferation and increased cellular apoptosis compared to WT CMECs.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Pharmacology & Pharmacy
Bingying Dai, Liqing Ding, Lijuan Zhao, Honglin Zhu, Hui Luo
Summary: This review summarizes the current knowledge on immune cells and stromal cells in SSc patients and discusses their potential roles in the pathogenesis of SSc, with a focus on recent advances in new subtypes by scRNA-seq.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Koki Yoshida, Osamu Uehara, Yoshihito Kurashige, Durga Paudel, Aya Onishi, Puja Neopane, Daichi Hiraki, Tetsuro Morikawa, Fumiya Harada, Rie Takai, Jun Sato, Masato Saitoh, Yoshihiro Abiko
Summary: The combination of a DNA demethylating agent and a histone deacetylase inhibitor can enhance the dedifferentiation of Malassez epithelial cell rests into stem-like cells, with the ability to differentiate into mesenchymal cells. This suggests a potential use for epigenetic agents in promoting periodontal tissue regeneration.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Biomedical
Solomiya Kyyak, Sebastian Blatt, Andreas Pabst, Daniel Thiem, Bilal Al-Nawas, Peer W. Kaemmerer
JOURNAL OF BIOMATERIALS APPLICATIONS
(2020)
Article
Dentistry, Oral Surgery & Medicine
Gregor Brandstetter, Sebastian Blatt, Jutta Goldschmitt, Louise Taylor, Paul Heymann, Bilal Al-Nawas, Thomas Ziebart
Summary: The study suggests that inhibition of MCT, especially MCT4, may help overcome radiation resistance in tumor cell lines. Experimental results show that MCT inhibitors have significant impacts on cell growth, migration, and cell viability after radiation therapy.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Dentistry, Oral Surgery & Medicine
D. G. E. Thiem, R. W. Frick, E. Goetze, M. Gielisch, B. Al-Nawas, P. W. Kaemmerer
Summary: In reconstructive surgery, flap monitoring is crucial for early identification of perfusion problems. This clinical study aimed to develop a non-invasive, objective approach for perfusion monitoring of free and pedicled flaps using hyperspectral imaging. The study found differences in parameters such as StO(2), NIR, THI, and TWI over time, with specific thresholds indicating arterial or venous perfusion issues. Results showed that pedicled flaps had decreased perfusion parameters compared to free flaps after transfer but remained similar later on.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Junho Jung, Jung Soo Park, Michel Dard, Bilal Al-Nawas, Yong-Dae Kwon
Summary: EMD-liquid combined with BCP bone substitute enhances new bone formation in a rabbit calvarial model. The EMD-liquid + BS group shows accelerated graft degradation and significantly increased new bone formation compared to the BS alone group. Further studies on graft materials are needed to optimize the regenerative potential of EMD-liquid.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Sebastian Blatt, Andreas Pabst, Stefanie Zimmer, Christian Walter, Bilal Al-Nawas, Maximilian Kruger
Summary: This study investigated a new rapid test for L1 antibody detection in HPV-16 associated oral squamous cell carcinoma (OSCC) and validated its diagnostic performance. The results showed that the rapid test for L1 antibodies demonstrated high sensitivity and specificity.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Engineering, Biomedical
Roxana Schwab, Martin Heller, Celine Pfeifer, Ronald E. Unger, Stefan Walenta, Sandra Nezi-Cahn, Bilal Al-Nawas, Annette Hasenburg, Walburgis Brenner
Summary: Tissue engineering of mucosa equivalents is crucial in the genitourinary region. The choice of scaffold is key in developing tissue engineered mucosa equivalents, with collagen scaffolds being considered the gold standard. The study found that TEOMs on the Bio-Gide (R) scaffold showed the best fibroblast differentiation and organization, making it a suitable matrix for mucosa substitutes.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Sebastian Blatt, Daniel G. E. Thiem, Andreas Pabst, Bilal Al-Nawas, Peer W. Kaemmerer
Summary: The combination of bone substitute materials (BSMs) with platelet-rich fibrin (PRF) showed significant positive pro-angiogenic effects, particularly with alloplastic and xenogeneic materials, leading to increased vessel formation in vivo. This demonstrates the enhanced angiogenetic potential of BSMs when combined with PRF, suggesting potential for bony regeneration in clinical applications.
Editorial Material
Dentistry, Oral Surgery & Medicine
Amely Hartmann, Michael Peetz, Bilal Al-Nawas, Marcus Seiler
CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
Roman Kia Rahimi-Nedjat, Andrea Tuettenberg, Keyvan Sagheb, Carmen Loquai, Benedict Rybczynski, Stephan Grabbe, Christian Walter, Bilal Al-Nawas
Summary: A retrospective study on head and neck cSCC patients identified previous skin cancer as a risk factor for recurrence, while histological factors like ulceration and tumor grading can accelerate relapse. Additionally, surrounding chronic precancerotic lesions and poor tumor grading were found to accelerate the observation of secondary cSCC.
JOURNAL OF CRANIO-MAXILLOFACIAL SURGERY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Eik Schiegnitz, Peer W. Kaemmerer, Philipp Hellwich, Jochem Koenig, Keyvan Sagheb, Bilal Al-Nawas
Summary: Comparing narrow-diameter implants (NDI) with standard-diameter implants in combination with horizontal bone augmentation procedures (SDI+A) for horizontally deficient alveolar ridges, results showed similar clinical outcomes, survival rates, and implant success rates after over 3 years of follow-up, indicating NDI as a reasonable alternative for horizontal bone atrophy cases.
CLINICAL ORAL IMPLANTS RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Matthias Gielisch, Maximilian Moergel, Bilal Al-Nawas, Peer W. Kaemmerer
Summary: This study found that the uptake of boron in HNSCC cells did not significantly increase with the trans-stimulation effect of L-tyrosine, but higher BPA concentrations in trans-stimulated cells led to higher intracellular boron concentrations. The impact of trans-stimulation on HNSCC seemed less relevant, but trans-stimulated cells showed a more distinct dose-dependent cellular boron uptake with higher BPA concentrations.
APPLIED SCIENCES-BASEL
(2021)
Article
Medicine, General & Internal
Daniel G. E. Thiem, Lukas Hans, Sebastian Blatt, Paul Romer, Diana Heimes, Bilal Al-Nawas, Peer W. Kaemmerer
Summary: This study investigated the dynamic skin perfusion after applying different combinations of local anesthetics using HSI. It was found that local anesthetics with different concentrations of epinephrine can lead to different vascular reaction patterns, resulting in varying skin perfusion trends.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Surgery
Till Kaemmerer, Tony Lesmeister, Victor Palarie, Eik Schiegnitz, Andrea Schroeter, Bilal Al-Nawas, Peer W. Kaemmerer
Summary: The study compared the osseointegration of CaP surfaces and SA surfaces in an in vivo minipig mandible model, showing a higher rate of osseointegration failure in CaP implants in the early phase post-insertion, but with decreasing differences between the two groups over time.
EUROPEAN SURGICAL RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
Daniel G. E. Thiem, Daniel Schneider, Michael Hammel, Bassam Saka, Bernhard Frerich, Bilal Al-Nawas, Peer W. Kammerer
Summary: Long-term complications after orthognathic surgery occurred more frequently than commonly described in the literature, and analyses of the quality of life show the need for more comprehensive preoperative patient education.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Alexander Volkmann, Helmut Hildebrandt, Stephan Grosse, Amely Hartmann, Keyvan Sagheb, Bilal Al-Nawas
Article
Engineering, Biomedical
Lin-Lin Luo, Jie Xu, Bing-Qiao Wang, Chen Chen, Xi Chen, Qiu-Mei Hu, Yu-Qiu Wang, Wan-Yun Zhang, Wan-Xiang Jiang, Xin-Ting Li, Hu Zhou, Xiao Xiao, Kai Zhao, Sen Lin
Summary: A novel AAV serotype, AAVYC5, introduced in this study, showed more efficient transduction into multiple retinal layers compared to AAV2, and enabled successful delivery of anti-angiogenic molecules in mice and non-human primates.