Article
Multidisciplinary Sciences
Bei Liu, Shi Chen, Yaxing Xu, Yulin Lyu, Jinlin Wang, Yuanyuan Du, Yongcheng Sun, Heming Liu, Haoying Zhou, Weifeng Lai, Anqi Xue, Ming Yin, Cheng Li, Yun Bai, Jun Xu, Hongkui Deng
Summary: The study introduces a chemically defined and xeno-free culture system for culturing and deriving human EPS cells, which can be long-term maintained in vitro with stable genetics and developmental potentials. This system also allows efficient derivation of human EPS cells from human fibroblast through reprogramming, showing promise for future biomedical applications.
NATURE COMMUNICATIONS
(2021)
Article
Neurosciences
Ethan T. Whitman, Siyuan Liu, Erin Torres, Allysa Warling, Kathleen Wilson, Ajay Nadig, Cassidy McDermott, Liv S. Clasen, Jonathan D. Blumenthal, Francois M. Lalonde, Stephen J. Gotts, Alex Martin, Armin Raznahan
Summary: Our study identified increased global resting-state functional connectivity in the left dorsolateral prefrontal cortex in XXY syndrome, which was partly linked to regional volumetric changes and severity of psychopathology. This provides biological insights into XXY syndrome as a disorder and a model of genetic risk for psychopathology.
Article
Genetics & Heredity
Kate Swanson, Juliet C. Bishop, Huda B. Al-Kouatly, Mona Makhamreh, Thomas Felton, Neeta L. Vora, Teresa N. Sparks, Angie C. Jelin
Summary: This study investigated the prenatal presentation of Klinefelter syndrome and found that prenatal diagnosis and genetic screening can identify fetal abnormalities, providing important information for birth planning and subsequent interventions.
PRENATAL DIAGNOSIS
(2023)
Article
Biochemistry & Molecular Biology
Jaakko Saari, Fatima Siddique, Sanna Korpela, Elina Mantyla, Teemu O. Ihalainen, Katri Kaukinen, Katriina Aalto-Setala, Katri Lindfors, Kati Juuti-Uusitalo
Summary: The small intestinal epithelium plays a crucial role in nutrition, drug absorption, and metabolism. By using animal-free components and serum-free method, functional small intestinal epithelial cells can be generated from iPSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Fernanda C. P. Mesquita, Eliel S. Leite, Jacquelynn Morrissey, Catarina Freitas, Tatiana Coelho-Sampaio, Camila Hochman-Mendez
Summary: Laminins (LNs) are important in cell-ECM interactions. PolyLN521, a self-polymerizing coating, can promote large-scale expansion of human iPSCs without affecting cell stability or pluripotency.
Article
Materials Science, Biomaterials
Ya-Chu Liu, Lee-Kiat Ban, Henry Hsin-Chung Lee, Hsin-Ting Lee, Yu-Tang Chang, Yun-Ting Lin, Her-Young Su, Shih-Tien Hsu, Akon Higuchi
Summary: The study revealed that supplementing optimal amounts of laminin-511 and rVT in the culture medium enabled successful cultivation of human pluripotent stem cells on uncoated dishes, promoting cell adhesion and maintaining pluripotency.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Genetics & Heredity
Line Dahl Jeppesen, Tina Duelund Hjortshoj, Johnny Hindkjaer, Lotte Hatt, Olav Bjorn Petersen, Ripudaman Singh, Palle Schelde, Lotte Andreasen, Rikke Christensen, Dorte L. Lildballe, Ida Vogel
Summary: The article introduces a cell-based noninvasive prenatal DNA testing method applied to twin pregnancies to assist couples in prenatal testing decision-making.
FRONTIERS IN GENETICS
(2022)
Article
Medicine, Research & Experimental
Ruslan Rust, Rebecca Z. Weber, Melanie Generali, Debora Kehl, Chantal Bodenmann, Daniela Uhr, Debora Wanner, Kathrin J. Zuercher, Hirohide Saito, Simon P. Hoerstrup, Roger M. Nitsch, Christian Tackenberg
Summary: In this study, transgene- and xeno-free neural progenitor cells (NPCs) were generated for potential clinical applications. These cells had stable gene expression, could be expanded, and spontaneously differentiated into mature neurons. This research presents a valuable tool for safe and effective cell therapies for brain injuries.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Yuka Sakata, Hong Zhang, Akiko Sugiyama, Nami Motosugi, Hiroshi Kimura, Yosuke Okamura, Atsushi Fukuda
Summary: High-resolution imaging analysis is essential for studying cell functions, but culturing cells on glass substrates often leads to cell instability. In this study, we coated the glass substrate with a nanosheet composed of hydrophobic polystyrene and Matrigel, which significantly improved the viability of human pluripotent stem cells (hPSCs). The nanosheet coating did not affect the transcriptome status of hPSC and allowed for high-resolution imaging assay. Our findings demonstrate that the nanosheet coating is beneficial for sensitive cells cultured on glass substrates.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Immunology
Francesca Panimolle, Claudio Tiberti, Matteo Spaziani, Gloria Riitano, Giuseppe Lucania, Antonella Anzuini, Andrea Lenzi, Daniele Gianfrilli, Maurizio Sorice, Antonio F. Radicioni
Summary: This study found significantly higher levels of non-organ-specific autoantibodies in patients with Klinefelter's syndrome compared to controls, while no such antibodies were detected in patients with rare higher-grade sex chromosome aneuploidies. This suggests that Klinefelter's syndrome and higher-grade sex chromosome aneuploidies should be considered as separate conditions.
CLINICAL AND EXPERIMENTAL IMMUNOLOGY
(2021)
Article
Genetics & Heredity
Luisa Ronzoni, Maria Francesca Bedeschi, Gaia Silibello, Veronica Accurti, Marina Di Segni, Valeria Nicotra, Paola Vizziello, Faustina Lalatta
Summary: This study highlights the unconfirmed increased risk of 47,XXY in cfDNA tests, suggesting the need for discussion on invasive testing to exclude more severe chromosomal variations.
PRENATAL DIAGNOSIS
(2021)
Review
Medicine, General & Internal
Jubara Alallah, Sohaib Habhab, Farzeen Mohtisham, Aiman Shawli, Mustafa Daghistani
Summary: This article reports the first case of a Saudi newborn with both Down syndrome and Klinefelter syndrome (double aneuploidy). The patient exhibited clinical features of Down syndrome, along with hypothyroidism and congenital heart disease.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2022)
Article
Cell & Tissue Engineering
Ying Hua, Kenji Yoshimochi, Junjun Li, Kazuhiro Takekita, Motoshi Shimotsuma, Lingjun Li, Xiang Qu, Jingbo Zhang, Yoshiki Sawa, Li Liu, Shigeru Miyagawa
Summary: In this study, a xeno-free ON2/AscleStem PSC medium was developed for the expansion of human-induced pluripotent stem cells (hiPSCs). It was found that hiPSCs cultured in this medium showed normal morphology, high self-renewal and differentiation abilities, and compatibility with different matrices. The hiPSCs grown on gelatin nanofiber showed enhanced growth and stemness. Compared to other media, ON2 medium exhibited higher pluripotency. This study demonstrates the potential of ON2 as a cost-effective and reliable medium for the long-term expansion and maintenance of hiPSCs.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Multidisciplinary Sciences
Eiko Shimizu, Hiroki Iguchi, Minh Nguyen Tuyet Le, Yuta Nakamura, Daigo Kobayashi, Yuhei Arai, Kenta Takakura, Seiko Benno, Noriko Yoshida, Masayoshi Tsukahara, Satoshi Haneda, Kouichi Hasegawa
Summary: In this study, a chemically synthetic culture substrate was developed for the expansion and differentiation of human pluripotent stem cells. The substrate exhibited thermotolerance and underwater stability, and could be stored at room temperature. It supported hPSC expansion with similar efficiency to commercial substrates and enabled directed differentiation.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Samuel Nayler, Devika Agarwal, Fabiola Curion, Rory Bowden, Esther B. E. Becker
Summary: By utilizing a robust method without the need for mouse co-culture, three-dimensional cerebellar organoids were successfully generated from human pluripotent stem cells (hPSCs), displaying hallmarks of in vivo cerebellar development. Single-cell profiling revealed the presence and maturity of transcriptionally distinct populations encompassing major cerebellar cell types. This xeno-free human cerebellar organoid model provides valuable insight into cerebellar development and associated disorders through enrichment of cerebellar disease genes in distinct cell populations.
SCIENTIFIC REPORTS
(2021)
Review
Endocrinology & Metabolism
Anne Jorgensen, Terje Svingen, Harriet Miles, Tarini Chetty, Jan-Bernd Stukenborg, Rod T. Mitchell
Summary: This article reviews the relationship between the development of the male reproductive system and DSD and TDS, and describes the experimental systems using animal and human tissues or cells that can be used to investigate the role of environmental factors in inducing male reproductive disorders. Finally, the evidence from experimental and epidemiologic approaches for the role of environmental factors in the development of specific male reproductive disorders, including cryptorchidism, hypospadias, and TGCC, is discussed.
HORMONE RESEARCH IN PAEDIATRICS
(2023)
Article
Immunology
Yi Chen, Shuwen You, Jie Li, Yifan Zhang, Georgia Kokaraki, Elisabeth Epstein, Joseph Carlson, Wen-Kuan Huang, Felix Haglund
Summary: Through studying the transcriptome features of endometrial cancer patients, we identified T follicular helper-like cells (Tfh) as an important factor with potential clinical application in anti-PD-1 treatment. We constructed a Tfh Infiltration Risk Model (TIRM) and found that it outperformed existing models in predicting the prognosis of EC patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Xichen Nie, Sarah K. Munyoki, Meena Sukhwani, Nina Schmid, Annika Missel, Benjamin R. Emery, DonorConnect, Jan-Bernd Stukenborg, Artur Mayerhofer, Kyle E. Orwig, Kenneth Aston, James M. Hotaling, Bradley R. Cairns, Jingtao Guo
Summary: This study utilized single-cell RNA-seq to investigate the effects of aging on testicular cells in men. The results revealed age-related changes in multiple cell types, including alterations in signaling pathways, inflammation, and metabolism. The extent of dysregulation was also found to be correlated with body mass index in older men. This research provides insights into the candidate molecular mechanisms underlying testicular changes caused by aging.
DEVELOPMENTAL CELL
(2022)
Article
Dermatology
Rania F. Zaarour, Dyuti Saha, Rakesh Dey, Anupam Dutta, Pankaj Kumar, Isha Rana, Ajai Pulianmackal, Abrar Rizvi, Namita Misra, Lionel Breton, Colin Jamora
Summary: The wound healing process consists of three phases: inflammation, proliferation, and remodeling. Substance P (SP), a neuropeptide secreted by peripheral neurons, plays a crucial role in mediating the interactions involved in tissue repair. It inhibits inflammatory responses, promotes proliferation-associated responses, and its effects on dermal fibroblasts diminish with donor age.
EXPERIMENTAL DERMATOLOGY
(2022)
Article
Oncology
Ioanna Xagoraris, Pedro Farrajota Neves da Silva, Georgia Kokaraki, Konstantina Stathopoulou, Bjoern Wahlin, Anders Osterborg, Nikolas Herold, Siok-Bian Ng, L. Jeffrey Medeiros, Elias Drakos, Birgitta Sander, George Z. Rassidakis
Summary: The expression of STING protein is restricted to T-cell and NK-cell lymphomas, and it has potential immunotherapeutic implications. These findings provide a new target and strategy for the treatment of lymphomas.
Article
Obstetrics & Gynecology
Mats Brannstrom, Pernilla Dahm-Kahler, Niclas Kvarnstrom, Anders Enskog, Jan I. Olofsson, Michael Olausson, Johan Molne, Randa Akouri, Stina Jarvholm, Lars Nilsson, Lennart Stigson, Henrik Hagberg, Hans Bokstrom
Summary: The first completed study of uterus transplantation (UTx) demonstrates that it is an effective treatment for infertility, resulting in the birth of healthy children and having only minor psychological and medical long-term effects on donors and recipients.
FERTILITY AND STERILITY
(2022)
Editorial Material
Cell & Tissue Engineering
Masahito Yoshihara, Magdalena Wagner, Anastasios Damdimopoulos, Cheng Zhao, Sophie Petropoulos, Shintaro Katayama, Juha Kere, Fredrik Lanner, Pauliina Damdimopoulou
Summary: Ovaries play a crucial role in the development, fertility, and reproduction of women. They age faster than other tissues and their function is lost before other organs undergo senescence. While there have been debates about the presence of germline stem cells and neo-oogenesis in adult human ovaries, our recent analysis did not find evidence of such stem cells. However, we welcome discussions on different protocols and acknowledge the importance of excellence in methods.
Editorial Material
Endocrinology & Metabolism
Swati Sharma, Jan-Bernd Stukenborg, Stefan Schlatt
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Dermatology
Isha Rana, Sunny Kataria, Tuan Lin Tan, Edries Yousaf Hajam, Deepak Kumar Kashyap, Dyuti Saha, Johan Ajnabi, Sayan Paul, Shashank Jayappa, Akhil S. H. P. Ananthan, Pankaj Kumar, Rania F. Zaarour, J. Haarshaadri, Gaurav Kansagara, Abrar Rizvi, Ravindra K. Zirmire, Krithika Badarinath, Sneha Uday Khedkar, Yogesh Chandra, Rekha Samuel, Renu George, Debashish Danda, Paul Mazhuvanchary Jacob, Rakesh Dey, Perundurai S. Dhandapany, You-Wen He, John Varga, Shyni Varghese, Colin Jamora
Summary: It was discovered that the transcription factor SNAI1 is overexpressed in the epidermis of patients with systemic sclerosis, and a transgenic mouse model mimicking this expression pattern can induce many clinical features of the human disease. The study found that Mindin protein plays a critical role in fibrogenesis by inducing early inflammatory cytokine production and collagen secretion in resident dermal fibroblasts. Targeting Mindin protein holds promise as an effective therapy for fibrosis.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Letter
Cell & Tissue Engineering
Masahito Yoshihara, Magdalena Wagner, Anastasios Damdimopoulos, Cheng Zhao, Sophie Petropoulos, Shintaro Katayama, Juha Kere, Fredrik Lanner, Pauliina Damdimopoulou
Review
Virology
Mohadese Hashem Boroojerdi, Tariq Al Jabry, Seyed Mohamad Javad Mirarefin, Halima Albalushi
Summary: Since December 2019, various strategies have been used to study the biology and pathogenesis of SARS-CoV-2 and discover effective treatments. One approach is using mini-organ technology to mimic COVID-19 and improve our understanding of the virus. Regenerative medicine research has shown the importance of organoid modeling in controlling COVID-19 through drug and therapeutic approach optimization.
Review
Multidisciplinary Sciences
Varsha Jain, Susana M. Chuva de Sousa M. Lopes, Mohammed A. Benotmane, Vittore Verratti, Rod T. Mitchell, Jan-Bernd Stukenborg
Summary: This review summarises key aspects of the first reproductive and developmental systems Science Community White Paper, supported by the European Space Agency (ESA). The white papers aim to reflect on the implications of space travel on the male and female reproductive systems and explore how this may impact society on Earth.
Article
Biology
Ahmed Al Ghaithi, Eyas Al Rashdi, Maryam Al Shukri, Rahma Al Ghabshi, Halima Albalushi
Summary: Improved treatments for cancer have increased survival rates, but they can also result in infertility for survivors. It is important for healthcare providers to discuss fertility preservation options with young cancer survivors, but in Oman, the lack of knowledge and availability of services hinders these discussions.
Article
Reproductive Biology
Rageia Elfageih, Ahmed Reda, Kristin Ros Kjartansdottir, Valentina Pampanini, Olle Soder, Jan -Bernd Stukenborg
Summary: This study aimed to investigate the impact of short-term hypothermic storage (4-8 degrees C) on prepubertal rat testicular tissue samples and found that the expression of genes related to energy, apoptosis, and angiogenesis pathways were altered after 12 and 24 h of storage.
REPRODUCTION AND FERTILITY
(2023)
Article
Biology
Chun-Ho Chan, Yin-Yu Lam, Nicodemus Wong, Lin Geng, Jilin Zhang, Virpi Ahola, Aman Zare, Ronald Adolphus Li, Fredrik Lanner, Wendy Keung, Yiu-Fai Cheung
Summary: This study reveals functional abnormalities in cardiomyocytes derived from Tetralogy of Fallot patients with DiGeorge syndrome, including impaired ventricular specification, altered cardiac gene expression, upregulated neural gene expression, and increased arrhythmogenicity. These findings are specific to patients with DiGeorge syndrome and have implications for their clinical outcomes.
COMMUNICATIONS BIOLOGY
(2023)