CRISPR/Cas9 facilitates genomic editing for large-scale functional studies in pluripotent stem cell cultures

One Step Into the Future: New iPSC Tools to Advance Research in Parkinson’s Disease and Neurological Disorders

(2019) Nguyen-Vi Mohamed et al.   Journal of Parkinsons Disease

Precisely controlling endogenous protein dosage in hPSCs and derivatives to model FOXG1 syndrome

(2019) Wenliang Zhu et al.   Nature Communications

CRISPR/Cas9 edited hiPSC-based vascular tissues to model aging and disease-dependent impairment

(2019) Aylin Acun et al.   TISSUE ENGINEERING PART A

CRISPR/Cas9: a powerful tool for identification of new targets for cancer treatment

(2019) Bin Liu et al.   DRUG DISCOVERY TODAY

CRISPR/Cas9-based epigenome editing: An overview of dCas9-based tools with special emphasis on off-target activity

(2019) Vanja Tadić et al.   METHODS

Genetic correction of induced pluripotent stem cells mediated by transcription activator-like effector nucleases targeting ALPL recovers enzyme activity and calcification in vitro

(2019) Chiho Nakano et al.   MOLECULAR GENETICS AND METABOLISM

Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/Cas9-mediated gene correction

(2019) Si Wang et al.   Protein & Cell

Estimation of clinical trial success rates and related parameters

(2018) Chi Heem Wong et al.   BIOSTATISTICS

CRISPR/Cas9 Gene editing of RyR2 in human stem cell-derived cardiomyocytes provides a novel approach in investigating dysfunctional Ca 2+ signaling

(2018) Hua Wei et al.   CELL CALCIUM

Genetic editing of colonic organoids provides a molecularly distinct and orthotopic preclinical model of serrated carcinogenesis

(2018) Tamsin R M Lannagan et al.   GUT

Comparison of Zinc Finger Nucleases Versus CRISPR-Specific Nucleases for Genome Editing of the Wiskott-Aldrich Syndrome Locus

(2018) Alejandra Gutierrez-Guerrero et al.   HUMAN GENE THERAPY

Induction of apoptosis in imatinib sensitive and resistant chronic myeloid leukemia cells by efficient disruption of bcr-abl oncogene with zinc finger nucleases

(2018) Ningshu Huang et al.   JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH

Targeting ectodysplasin promotor by CRISPR/dCas9-effector effectively induces the reprogramming of human bone marrow-derived mesenchymal stem cells into sweat gland-like cells

(2018) Sujing Sun et al.   Stem Cell Research & Therapy

Functional Assessment of Patient-Derived Retinal Pigment Epithelial Cells Edited by CRISPR/Cas9

(2018) Leah Foltz et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus

(2018) Stefanie Schuster et al.   Stem Cell Research

CRISPR/Cas9 editing of APP C-terminus attenuates β-cleavage and promotes α-cleavage

(2018) Jichao Sun et al.   Nature Communications

The Impact of CRISPR/Cas9 Technology on Cardiac Research: From Disease Modelling to Therapeutic Approaches

(2017) Benedetta M. Motta et al.   Stem Cells International

CRISPR/Cas9 Genome-Editing System in Human Stem Cells: Current Status and Future Prospects

(2017) Zhao Zhang et al.   Molecular Therapy-Nucleic Acids

CRISPR/Cas9 Immune System as a Tool for Genome Engineering

(2016) Magdalena Hryhorowicz et al.   ARCHIVUM IMMUNOLOGIAE ET THERAPIAE EXPERIMENTALIS

Reverse engineering human neurodegenerative disease using pluripotent stem cell technology

(2016) Ying Liu et al.   BRAIN RESEARCH

CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs

(2016) Mohammad A. Mandegar et al.   Cell Stem Cell

CRISPR guide RNA design for research applications

(2016) Stephanie E. Mohr et al.   FEBS Journal

Applications of CRISPR technologies in research and beyond

(2016) Rodolphe Barrangou et al.   NATURE BIOTECHNOLOGY

An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting

(2016) Jian Cao et al.   NUCLEIC ACIDS RESEARCH

Targeted genome engineering using designer nucleases: State of the art and practical guidance for application in human pluripotent stem cells

(2016) Sylvia Merkert et al.   Stem Cell Research

On the Origin of CRISPR-Cas Technology: From Prokaryotes to Mammals

(2016) Francisco J.M. Mojica et al.   TRENDS IN MICROBIOLOGY

TALENs Facilitate Single-step Seamless SDF Correction of F508del CFTR in Airway Epithelial Submucosal Gland Cell-derived CF-iPSCs

(2016) Shingo Suzuki et al.   Molecular Therapy-Nucleic Acids

The CRISPR-Cas immune system: Biology, mechanisms and applications

(2015) Devashish Rath et al.   BIOCHIMIE

CRISPR genome engineering and viral gene delivery: A case of mutual attraction

(2015) Florian Schmidt et al.   Biotechnology Journal

Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9

(2015) Yuejun Chen et al.   Cell Stem Cell

Stem Cell Therapies in Clinical Trials: Progress and Challenges

(2015) Alan Trounson et al.   Cell Stem Cell

The roles of CRISPR–Cas systems in adaptive immunity and beyond

(2015) Rodolphe Barrangou   CURRENT OPINION IN IMMUNOLOGY

Systematic analysis of CRISPR–Cas9 mismatch tolerance reveals low levels of off-target activity

(2015) Emily M. Anderson et al.   JOURNAL OF BIOTECHNOLOGY

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

(2015) Ayal Hendel et al.   NATURE BIOTECHNOLOGY

Therapeutic genome editing: prospects and challenges

(2015) David Benjamin Turitz Cox et al.   NATURE MEDICINE

Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids

(2015) Benjamin S. Freedman et al.   Nature Communications

Allogenic iPSC-derived RPE cell transplants induce immune response in pigs: a pilot study

(2015) Elliott H Sohn et al.   Scientific Reports

CRISPR genome engineering and viral gene delivery: A case of mutual attraction

(2015) Florian Schmidt et al.   Biotechnology Journal

A quick guide to CRISPR sgRNA design tools

(2015) Vincent A Brazelton et al.   GM Crops & Food-Biotechnology in Agriculture and the Food Chain

Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9

(2014) Pankaj K. Mandal et al.   Cell Stem Cell

m6A RNA Modification Controls Cell Fate Transition in Mammalian Embryonic Stem Cells

(2014) Pedro J. Batista et al.   Cell Stem Cell

Human Pluripotent Stem Cell Culture: Considerations for Maintenance, Expansion, and Therapeutics

(2014) Kevin G. Chen et al.   Cell Stem Cell

CRISPR/Cas9 for genome editing: progress, implications and challenges

(2014) F. Zhang et al.   HUMAN MOLECULAR GENETICS

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

(2014) Carolin Anders et al.   NATURE

Investigating human disease using stem cell models

(2014) Jared L. Sterneckert et al.   NATURE REVIEWS GENETICS

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Integrating Human Pluripotent Stem Cells into Drug Development

(2013) Sandra J. Engle et al.   Cell Stem Cell

Enhanced Efficiency of Human Pluripotent Stem Cell Genome Editing through Replacing TALENs with CRISPRs

(2013) Qiurong Ding et al.   Cell Stem Cell

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

(2013) Ophir Shalem et al.   SCIENCE

Cytotoxic Chromosomal Targeting by CRISPR/Cas Systems Can Reshape Bacterial Genomes and Expel or Remodel Pathogenicity Islands

(2013) Reuben B. Vercoe et al.   PLoS Genetics

RNA-programmed genome editing in human cells

(2013) Martin Jinek et al.   eLife

Direct Comparison of Autologous and Allogeneic Transplantation of iPSC-Derived Neural Cells in the Brain of a Nonhuman Primate

(2013) Asuka Morizane et al.   Stem Cell Reports

The Promise and Perils of Stem Cell Therapeutics

(2012) George Q. Daley   Cell Stem Cell

A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models

(2012) Qiurong Ding et al.   Cell Stem Cell

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Genome Engineering With Zinc-Finger Nucleases

(2011) Dana Carroll   GENETICS

Genetic engineering of human pluripotent cells using TALE nucleases

(2011) Dirk Hockemeyer et al.   NATURE BIOTECHNOLOGY

Evolution and classification of the CRISPR–Cas systems

(2011) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

(2010) M. Christian et al.   GENETICS

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures

(2010) Yannick Doyon et al.   NATURE METHODS

Genome editing with engineered zinc finger nucleases

(2010) Fyodor D. Urnov et al.   NATURE REVIEWS GENETICS

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

Rapid “Open-Source” Engineering of Customized Zinc-Finger Nucleases for Highly Efficient Gene Modification

(2008) Morgan L. Maeder et al.   MOLECULAR CELL

Unexpected failure rates for modular assembly of engineered zinc fingers

(2008) Cherie L Ramirez et al.   NATURE METHODS

DNA-binding Specificity Is a Major Determinant of the Activity and Toxicity of Zinc-finger Nucleases

(2007) Tatjana I Cornu et al.   MOLECULAR THERAPY