Journal
BIOMACROMOLECULES
Volume 17, Issue 12, Pages 3964-3972Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.biomac.6b01356
Keywords
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Funding
- National Institutes of Health [5R21EB016947-02]
- Division of Chemistry (CHE), National Science Foundation [NSF/CHE-1346572]
- Division of Materials Research (DMR), National Science Foundation [NSF/CHE-1346572]
- U.S. DOE [DE-AC02-06CH11357]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1346572] Funding Source: National Science Foundation
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Coiled-coil peptide polymer conjugates are an emerging class of biomaterials. Fundamental understanding of the coiled-coil oligomeric state and assembly process of these hybrid building blocks is necessary to exert control over their assembly into well-defined structures. Here, we studied the effect of peptide structure and PEGylation on the self-assembly process and oligomeric state of a Langmuir monolayer of amphiphilic coiled-coil peptide polymer conjugates using X-ray reflectivity (XR) and grazing-incidence X-ray diffraction (GIRD). Our results show that the oligomeric state of PEGylated amphiphiles based on 3-helix bundle-forming peptide is surface pressure dependent, a mixture of dimers and trimers was formed at intermediate surface pressure but transitions into trimers completely upon increasing surface pressure. Moreover, the interhelical distance within the coiled-coil bundle of 3-helix peptide-PEG conjugate amphiphiles was not perturbed under high surface pressure. Present studies provide valuable insights into the self-assembly process of hybrid peptide polymer conjugates and guidance to develop biomaterials with controlled multivalency of ligand presentation.
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