Article
Reproductive Biology
Nirvay Sah, Claire Stenhouse, Katherine M. Halloran, Robyn M. Moses, Heewon Seo, Robert C. Burghardt, Gregory A. Johnson, Guoyao Wu, Fuller W. Bazer
Summary: In this study, the metabolism of creatine (Cr) at the uterine-conceptus interface in sheep during early gestation was investigated. The results showed an increase in the levels of Cr and guanidinoacetate (GA) between Days 12 and 17 of pregnancy. The expression of mRNAs related to GA formation, Cr synthesis, and Cr/PCr utilization was higher on Days 17 and 21 compared to earlier days of pregnancy. These findings provide insights into the specific metabolism of Cr at the uterine-conceptus interface and suggest a role for the Cr-CK-PCr system in ovine conceptus development and implantation.
BIOLOGY OF REPRODUCTION
(2022)
Article
Reproductive Biology
Eleanore O'Neil, Thomas E. Spencer
Summary: This study found that certain lipid components are reduced in the endometrium of pregnant ewes, while certain lipid compounds are elevated in the uterine lumen. Additionally, elevated levels of amino acids, carbohydrates, and energy substrates were observed in the uterine lumen of pregnant ewes.
BIOLOGY OF REPRODUCTION
(2021)
Article
Reproductive Biology
Hwanhee Jang, Soohyung Lee, Inkyu Yoo, Yohan Choi, Jisoo Han, Yugyeong Cheon, Hakhyun Ka
Summary: S100A8, S100A9, and S100A12, calcium-binding proteins, are dynamically expressed in response to conceptus-derived signals at the maternal-conceptus interface, potentially playing a critical role in regulating endometrial epithelial cell function and conceptus implantation to support the establishment and maintenance of pregnancy in pigs.
BIOLOGY OF REPRODUCTION
(2022)
Article
Reproductive Biology
Claire Stenhouse, Katherine M. Halloran, Makenzie G. Newton, Robyn M. Moses, Nirvay Sah, Larry J. Suva, Dana Gaddy, Fuller W. Bazer
Summary: Tissue-nonspecific alkaline phosphatase (TNSALP) plays a critical role in phosphate transport and homeostasis at the maternal-conceptus interface in ruminants.
BIOLOGY OF REPRODUCTION
(2023)
Article
Reproductive Biology
Victoria de Brun, Juan J. Loor, Hugo Naya, Andrea Grana-Baumgartner, Mario Vailati-Riboni, Omar Bulgari, Khuram Shahzad, Jose Alfonso Abecia, Cecilia Sosa, Ana Meikle
Summary: Transcriptomics and bioinformatics were used to study the impact of undernutrition and pregnancy on uterine immune system and remodeling. Results showed that pregnancy altered gene expression, particularly immune system and uterine remodeling genes, while undernutrition attenuated these effects.
Article
Agriculture, Dairy & Animal Science
Matthew R. Romoser, Katie L. Bidne, Lance H. Baumgard, Aileen F. Keating, Jason W. Ross
Summary: Heat stress has significant economic effects on animal agriculture, and mitigation strategies are necessary. This study found that heat stress may affect luteal development before pregnancy establishment, and ALT supplementation can advance conceptus elongation.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Reproductive Biology
Nirvay Sah, Claire Stenhouse, Katherine M. Halloran, Robyn M. Moses, Heewon Seo, Robert C. Burghardt, Gregory A. Johnson, Guoyao Wu, Fuller W. Bazer
Summary: Creatine and its related enzymes play a role in fetal-placental development in sheep. The creatine-creatine kinase-phosphocreatine system is involved in maintaining ATP levels in the placenta. This study found that gestational age affects the concentrations of creatine and guanidinoacetate, as well as the expression of related mRNAs and proteins in uterine and placental tissues. These findings suggest that the creatine-creatine kinase-phosphocreatine system is important for energy homeostasis during fetal-placental development.
BIOLOGY OF REPRODUCTION
(2023)
Article
Cell Biology
Emmalee J. Northrop-Albrecht, Jerica J. J. Rich, Robert A. Cushman, Runan Yao, Xijin Ge, George A. Perry
Summary: The interaction between preovulatory estradiol and the conceptus induces the expression of genes, proteins, and pathways necessary for pregnancy.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Article
Reproductive Biology
Sudikshya Paudel, Bangmin Liu, Magdalina J. Cummings, Kelsey E. Quinn, Fuller W. Bazer, Kathleen M. Caron, Xiaoqiu Wang
Summary: The study revealed higher levels of ADM in uterine fluid of pregnant pigs, indicating its significant role in implantation and conceptus growth. Additionally, the increased expression of CALCRL, RAMP2, and ACKR3 in pregnant pig conceptuses suggests the important influence of ADM on uterine receptivity and embryo development during pregnancy.
BIOLOGY OF REPRODUCTION
(2021)
Article
Reproductive Biology
Robyn M. Moses, Katherine M. Halloran, Claire Stenhouse, Nirvay Sah, Avery C. Kramer, Bryan A. McLendon, Heewon Seo, Gregory A. Johnson, Guoyao Wu, Fuller W. Bazer
Summary: Fructose is an important metabolic substrate for ovine conceptuses, as it can enter the pentose cycle and tricarboxylic acid cycle for metabolism. Lactate is the primary product of glycolysis from fructose under oxygenated and hypoxic conditions.
BIOLOGY OF REPRODUCTION
(2022)
Article
Reproductive Biology
James W. Frank, Chelsie B. Steinhauser, Xiaoqiu Wang, Robert C. Burghardt, Fuller W. Bazer, Greg A. Johnson
Summary: During the peri-implantation period of pregnancy in sheep, blocking ITGB3 expression in conceptuses did not prevent elongation and implantation, but decreased embryo growth and expression of SPP1 and NOS3. ITGB3 may play a role in regulating vasculature development within the allantois, impacting nutrient transport and embryo growth.
BIOLOGY OF REPRODUCTION
(2021)
Article
Pharmacology & Pharmacy
Annamaria Schaffer, Judit Hajagos-Toth, Eszter Ducza, Nikolett Bodi, Maria Bagyanszki, Zita Szalai, Robert Gaspar
Summary: The study investigated the effects of the KISS1 94-121 fragment on rat uterine contractility during pregnancy, and found that the expression of Kiss1r decreases towards the end of gestation. The majority of Kiss1r is located in the myometrium, and the relaxation effect of kisspeptin decreases towards the end of gestation in parallel with Kiss1r expression reduction. These findings suggest a potential role of kisspeptin in maintaining uterine quiescence with implications in miscarriage or preterm contractions.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Immunology
Jiri Fronek, Libor Janousek, Jakub Kristek, Jaroslav Chlupac, Marek Pluta, Robert Novotny, Jana Maluskova, Michael Olausson
Summary: This study reported the first live birth after a deceased donor uterus transplant in Europe, demonstrating that nulliparous uteri may be a suitable source of uterine grafts for transplantation. Stenting appears to be a feasible treatment for vaginal anastomotic stenosis.
Article
Biology
Ran Li, Peng Yang, Ming Li, Wenwen Fang, Xiangpeng Yue, Hojjat Asadollahpour Nanaei, Shangquan Gan, Duo Du, Yudong Cai, Xuelei Dai, Qimeng Yang, Chunna Cao, Weidong Deng, Sangang He, Wenrong Li, Runlin Ma, Mingjun Liu, Yu Jiang
Summary: The study presents a novel genome assembly of the Hu sheep and the first draft assembly of ovine Y chromosome, revealing three major paternal lineages and a new lineage in domestic sheep. It suggests that wild Iranian mouflons have introgressed into domestic sheep populations in East Asia and Africa, introducing a specific lineage carrying HY2.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Cell Biology
E. O'Callaghan, J. M. Sanchez, M. B. Rabaglino, M. McDonald, H. Liu, T. E. Spencer, S. Fair, D. A. Kenny, P. Lonergan
Summary: The study found that conceptuses with high fertility status can stimulate multiple pathways involved in immune response, similar to IFNT treatment, while conceptuses with low fertility status apparently have no effect on these pathways.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Reproductive Biology
Gregory W. Burns, Kelsey E. Brooks, Eleanore V. O'Neil, Darren E. Hagen, Susanta K. Behura, Thomas E. Spencer
BIOLOGY OF REPRODUCTION
(2018)
Article
Biochemistry & Molecular Biology
Andrew M. Kelleher, Susanta K. Behura, Gregory W. Burns, Steven L. Young, Francesco J. DeMayo, Thomas E. Spencer
Article
Biotechnology & Applied Microbiology
Jennifer N. Kiser, Elizabeth M. Keuter, Christopher M. Seabury, Mahesh Neupane, Joao G. N. Moraes, Joseph Dalton, Gregory W. Burns, Thomas E. Spencer, Holly L. Neibergs
Article
Biotechnology & Applied Microbiology
Jennifer N. Kiser, Erin Clancey, Joao G. N. Moraes, Joseph Dalton, Gregory W. Burns, Thomas E. Spencer, Holly L. Neibergs
Article
Biochemistry & Molecular Biology
Eleanore O'Neil, Kelsey Brooks, Gregory W. Burns, Martha S. Ortega, Anna C. Denicol, Luis H. Aguiar, Gabriela H. Pedroza, Joshua Benne, Thomas E. Spencer
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2020)
Article
Genetics & Heredity
Kayleen F. Oliver, Alexandria M. Wahl, Mataya Dick, Jewel A. Toenges, Jennifer N. Kiser, Justine M. Galliou, Joao G. N. Moraes, Gregory W. Burns, Joseph Dalton, Thomas E. Spencer, Holly L. Neibergs
Article
Reproductive Biology
Eleanore O'Neil, Gregory W. Burns, Christina R. Ferreira, Thomas E. Spencer
BIOLOGY OF REPRODUCTION
(2020)
Article
Reproductive Biology
Eleanore O'Neil, Gregory W. Burns, Thomas E. Spencer
Article
Biochemistry & Molecular Biology
Irene Malo Estepa, Haidee Tinning, Elton Jose Rosas Vasconcelos, Beatriz Fernandez-Fuertes, Jose Maria Sanchez, Gregory W. Burns, Thomas E. Spencer, Pat Lonergan, Niamh Forde
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Genetics & Heredity
Justine M. Galliou, Jennifer N. Kiser, Kayleen F. Oliver, Christopher M. Seabury, Joao G. N. Moraes, Gregory W. Burns, Thomas E. Spencer, Joseph Dalton, Holly L. Neibergs
Article
Biochemistry & Molecular Biology
Emmanuel N. Paul, Gregory W. Burns, Tyler J. Carpenter, Joshua A. Grey, Asgerally T. Fazleabas, Jose M. Teixeira
Summary: Transcriptome analysis revealed significant phenotypic and gene expression differences between fibroid-associated myometrium and non-diseased myometrium, particularly in upregulated genes related to extracellular matrix and structure organization. These results suggest that fibroid studies should consider using both matched myometrium and non-diseased myometrium as controls to better understand the pathophysiology of uterine fibroids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Chelsie B. Steinhauser, Colleen A. Lambo, Katharine Askelson, Gregory W. Burns, Susanta K. Behura, Thomas E. Spencer, Fuller W. Bazer, Michael Carey Satterfield
Summary: Placental development is modified in response to maternal nutrient restriction, leading to different fetal growth rates. Differences in gene expression and function were found between SGA and NR NonSGA placentomes, with overrepresentation of genes related to natural-killer-cell-mediated cytotoxicity in SGA placentomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Emmanuel N. Paul, Joshua A. Grey, Tyler J. Carpenter, Zachary B. Madaj, Kin H. Lau, Scott A. Givan, Gregory W. Burns, Ronald L. Chandler, Ganesa R. Wegienka, Hui Shen, Jose M. Teixeira
Summary: Uterine fibroids disproportionately affect Black women compared to women of other races and ethnicities. This study examined the molecular profiles of myometrium and fibroids from Black and White women, and found differences in gene expression and DNA methylation patterns between the two races. The results suggest that the molecular basis for the racial disparity in uterine fibroids may lie in the myometrium rather than the fibroids themselves.
Article
Obstetrics & Gynecology
Kentaro Kai, Niraj R. Joshi, Gregory W. Burns, Samantha M. Hrbek, Erin L. Vegter, Maria Ariadna Ochoa-Bernal, Yong Song, Genna E. Moldovan, Lorenzo F. Sempere, Eduardo H. Miyadahira, Paulo C. Serafini, Asgerally T. Fazleabas
Summary: This study explores the role of miR-210 and its targets, IGFBP3 and COL8A1, in the growth and development of endometriosis. It was found that miR-210 expression was decreased in ectopic lesions, while IGFBP3 and COL8A1 expression was increased. MiR-210 overexpression suppressed IGFBP3 expression and attenuated cell proliferation and migration.
REPRODUCTIVE SCIENCES
(2023)
Article
Medicine, Research & Experimental
Yong Song, Gregory W. Burns, Niraj R. Joshi, Ripla Arora, J. Julie Kim, Asgerally T. Fazleabas
Summary: The development and progression of endometriotic lesions are poorly understood, but immune cell dysfunction and inflammation are closely associated with the pathophysiology of endometriosis. In this study, a 3D in vitro model using endometriotic spheroids (ES) was developed to explore the role of epithelial-stromal interactions and model peritoneal invasion associated with lesion development. Transcriptomic analysis revealed inflammation-related pathways and significant overlap with baboon endometriotic lesions. Furthermore, a model with human peritoneal mesothelial cells in an extracellular matrix was developed to mimic invasion of endometrial tissue into the peritoneum.