Review
Cell Biology
Sadie VanHorn, Samantha A. Morris
Summary: Lineage tracing and fate mapping are two disciplines for tracking cells and their progeny, with lineage tracing focusing on identifying progeny and fate maps showing embryo development. Recent genomic technologies have enabled high-resolution reconstruction of lineage relationships. Fate maps, however, offer spatial information often lost in lineage reconstruction.
DEVELOPMENTAL CELL
(2021)
Review
Urology & Nephrology
Yoshiharu Muto, Benjamin D. Humphreys
Summary: Lineage tracing was originally developed to identify all progeny of a single cell during morphogenesis, but has since been applied to organ homeostasis and injury recovery. Modern techniques rely on reporter gene expression induced by cell-specific DNA recombination, and recent advances include intersectional genetics and sequencing-based genomic lineage tracing.
KIDNEY INTERNATIONAL
(2021)
Review
Neurosciences
Jorge Garcia-Marques, Isabel Espinosa-Medina, Tzumin Lee
Summary: Reconstructing the genealogy of cells in an organism is a challenging task in developmental biology, with implications for understanding normal and pathological development. Various strategies have been used for lineage tracing, from direct observation to genetic approaches. The achievements and limitations of past and current technology have been discussed, along with speculation on reaching future milestones in the field.
PROGRESS IN NEUROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Mingze Yao, Tinglin Ren, Yuanqing Pan, Xiaoqing Xue, Rong Li, Lei Zhang, Yuhang Li, Ke Huang
Summary: This review presents the progress of the latest CbLT (CRISPR-based Lineage Tracing) and discusses the current limitations and potential technical pitfalls in their application and other emerging concepts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Developmental Biology
Steffen Wolf, Yinan Wan, Katie McDole
Summary: Advances in light microscopy, labelling strategies and computational methods have enabled detailed investigations into cell fates, and when combined with genomics and single-cell transcriptomics, developmental biology can describe embryo formation in unprecedented detail. The review discusses different strategies and applications for lineage tracing in live-imaging data, as well as outlining software methodologies for various cell-tracking challenges.
Article
Immunology
Moujtaba Y. Kasmani, Ryan Zander, H. Kay Chung, Yao Chen, Achia Khatun, Martina Damo, Paytsar Topchyan, Kaitlin E. Johnson, Darya Levashova, Robert Burns, Ulrike M. Lorenz, Vera L. Tarakanova, Nikhil S. Joshi, Susan M. Kaech, Weiguo Cui
Summary: Using single-cell RNA and TCR sequencing, researchers investigated the developmental relationships and fate decisions of CD8(+) T cells during chronic infection. They found substantial clonal and phenotypic diversity, and identified a subset of intermediate cells that can differentiate into terminal effector and exhausted cells. Type I IFN and IRF7 were found to drive exhaustion, while Zeb2 was critical for effector cell generation. TCR avidity correlated with exhausted fate and SHP-1 selectively restricted accumulation of low-avidity effector cells.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Theresa B. Loveless, Joseph H. Grotts, Mason W. Schechter, Elmira Forouzmand, Courtney K. Carlson, Bijan S. Agahi, Guohao Liang, Michelle Ficht, Beide Liu, Xiaohui Xie, Chang C. Liu
Summary: CHYRON is a novel DNA recorder that primarily writes new DNA by repeatedly inserting random nucleotides at a single locus in temporal order, instead of erasing DNA like existing recorders. By combining Cas9, a homing guide RNA, and template-independent DNA polymerase, CHYRON has been successfully applied as an evolving lineage tracer and a recorder of user-selected cellular stimuli.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Laleh Haghverdi, Leif S. Ludwig
Summary: The concept of cell fate is about the future identity of a cell and its daughters through cell differentiation and division. Understanding and manipulating cell fate has been a long-standing goal in developmental and regenerative biology. Recent advancements in single-cell genomics and integrative lineage-tracing approaches have provided insights into the molecular features that can predict cell fate.
Review
Cardiac & Cardiovascular Systems
Tao Wang, Xinzhe Chen, Kai Wang, Jie Ju, Xue Yu, Shaocong Wang, Cuiyun Liu, Kun Wang
Summary: The Cre-loxP-mediated genetic lineage tracing system is crucial for tracking the fate of single-cell progeny or cell populations. Understanding the hierarchy of cardiac progenitor cells helps to uncover cell fate and origin in cardiac development. Several prospective Cre-loxP-based lineage-tracing systems have been utilized to precisely analyze the fate determination and developmental characteristics of different cardiac cell types.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Cell Biology
Jingjing Zhou, Xiangxiang Jiang, Haowei Wu, Lianjun Zhang, Min Chen, Zhiming Shen, Xudong Guo, Hongmei Wang, Fei Gao
Summary: In this study, the cell fate of Foxl2-expressing cells in fetal ovaries was analyzed using lineage tracing and single-cell transcriptomics. It was found that these cells give rise to granulosa cells, theca-interstitial cells, and stromal cells at later developmental stages. This study provides important insights into the differentiation of somatic cells in ovary development.
Article
Cell & Tissue Engineering
Chase A. Pagani, Amanda K. Huber, Charles Hwang, Simone Marini, Karthik Padmanabhan, Nicholas Livingston, Johanna Nunez, Yuxiao Sun, Nicole Edwards, Yu-Hao Cheng, Noelle Visser, Pauline Yu, Nicole Patel, Joseph A. Greenstein, Husain Rasheed, Reagan Nelson, Karen Kessel, Kaetlin Vasquez, Amy L. Strong, Geoffrey E. Hespe, Jane Y. Song, Deneen M. Wellik, Benjamin Levi
Summary: The research discovered a specific lineage of cells as ectopic bone progenitors in the zeugopod, providing a new perspective on fate specification and multipotency acquired after injury.
Article
Cell Biology
Camila B. Lopez-Anido, Anne Vaten, Nicole K. Smoot, Nidhi Sharma, Victoria Guo, Yan Gong, M. Ximena Anleu Gil, Annika K. Weimer, Dominique C. Bergmann
Summary: The study investigates the development and regulation of stomatal lineage in Arabidopsis leaf epidermis, revealing detailed cell differentiation processes using single-cell transcriptomics and genetics. It identifies the extended role of transcriptional regulator in reinforcing cell fate commitment through integration of genome-scale datasets and precise functional manipulations.
DEVELOPMENTAL CELL
(2021)
Review
Clinical Neurology
Daisuke Ito, Satoru Morimoto, Shinichi Takahashi, Kensuke Okada, Jin Nakahara, Hideyuki Okano
Summary: Significant progress has been made in drug screening for amyotrophic lateral sclerosis using patient-derived induced pluripotent stem cells, leading to the identification of candidate drugs and the advancement of clinical trials. This stem cell-based approach shows safety and positive effects, reducing costs and time compared to animal testing.
Article
Andrology
Yoshitaka Fujihara, Kiyonori Kobayashi, Ferheen Abbasi, Tsutomu Endo, Zhifeng Yu, Masahito Ikawa, Martin M. Matzuk
Summary: In this study, Pdcl2 knockout mice were generated to investigate the essential roles of PDCL2 in mammalian reproduction. The findings showed that PDCL2 is crucial for sperm acrosome development and male fertility, suggesting it as a potential contraceptive target in men.
Article
Biotechnology & Applied Microbiology
Ryota Tamura, Hiroyuki Miyoshi, Kent Imaizumi, Masahiro Yo, Yoshitaka Kase, Tsukika Sato, Mizuto Sato, Yukina Morimoto, Oltea Sampetrean, Jun Kohyama, Munehisa Shinozaki, Atsushi Miyawaki, Kazunari Yoshida, Hideyuki Saya, Hideyuki Okano, Masahiro Toda
Summary: This study demonstrates the potential benefit of gene-edited induced pluripotent stem cells (iPSCs) for the treatment of invasive glioma stem cells (GSCs). Neural stem cells (NSCs) derived from CRISPR/Cas9-edited iPSCs expressing a suicide gene showed enhanced tumor-trophic migratory capacity and significant in vivo antitumor effects. The study also identified key signaling pathways and gene expressions associated with the efficacy of therapeutic iPSC-NSCs.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Cell Biology
Satoru Morimoto, Koichi Saeki, Masaru Takeshita, Kunio Hirano, Mariko Shirakawa, Yumiko Yamada, Shiho Nakamura, Fumiko Ozawa, Hideyuki Okano
Summary: The study developed an intranasal vaccine for COVID-19 by using a Sendai virus vector loaded with the receptor binding domain of SARS-CoV-2 as an antigen. The vaccine induced a strong immune response, with increased levels of S-RBD-specific antibodies in serum and bronchoalveolar lavage fluid. The booster dose further enhanced the production of neutralizing antibodies, indicating the effectiveness of the vaccine.
Article
Cell Biology
Sho Yoshimatsu, Mayutaka Nakajima, Iki Sonn, Rie Natsume, Kenji Sakimura, Ena Nakatsukasa, Toshikuni Sasaoka, Mari Nakamura, Takashi Serizawa, Tsukika Sato, Erika Sasaki, Hongkui Deng, Hideyuki Okano
Summary: This study compared the gene expression profiles of mouse, human, and common marmoset EPSCs using transcriptomic approaches. The marmoset EPSC-like cells displayed a distinct morphology but exhibited a pluripotent state similar to embryonic stem cells. They also showed interspecies chimeric contribution to mouse embryos. Furthermore, the gene expression of the marmoset EPSC-like cells resembled that of human EPSCs. Overall, this study evaluated the efficacy of the method for deriving marmoset EPSCs.
Article
Biology
Atsushi Taniguchi, Yukinori Nishigami, Hiroko Kajiura-Kobayashi, Daisuke Takao, Daisuke Tamaoki, Toshiyuki Nakagaki, Shigenori Nonaka, Seiji Sonobe
Summary: Amoebae are crucial for the carbon cycle and understanding their behavior is important for global environment. By studying Amoeba Proteus, researchers found that the whole cell membrane moves during pressure-driven locomotion and the dorsal cell membrane moves more slowly than the other membrane. The membrane complexity is also asymmetrical between the dorsal and ventral sides.
Article
Engineering, Biomedical
Shogo Hashimoto, Narihito Nagoshi, Munehisa Shinozaki, Katsuyuki Nakanishi, Yu Suematsu, Takahiro Shibata, Momotaro Kawai, Takahiro Kitagawa, Kentaro Ago, Yasuhiro Kamata, Kaori Yasutake, Ikuko Koya, Yoshinari Ando, Aki Minoda, Tomoko Shindo, Shinsuke Shibata, Morio Matsumoto, Masaya Nakamura, Hideyuki Okano
Summary: In this study, the therapeutic effect of environmental modulation on severe chronic spinal cord injury (SCI) was explored. The use of a clinically relevant hepatocyte growth factor (HGF)-releasing scaffold and human iPS cell-derived neural stem/progenitor cell (hNS/PCs) transplantation led to functional recovery. This study provides evidence for the feasibility of regenerative medicine strategies using clinically relevant materials in the treatment of severe chronic SCI.
Article
Developmental Biology
Rena Kashimoto, Yasuhiro Kamei, Shigenori Nonaka, Yohei Kondo, Sakiya Yamamoto, Saya Furukawa, Ayaka Ohashi, Akira Satoh
Summary: Our study revealed that the lattice structure of dermal collagen fibers in axolotls is not fully regenerated after skin injury, but can be induced by nerves. The mechanism of collagen fiber regeneration remains unknown. We found that cells involved in skin repair/regeneration are derived from both the surrounding dermis and subcutaneous tissue, and can braid collagen fibers with the presence of nerves. Additionally, FGF signaling can substitute for nerve roles in the conversion of subcutaneous fibroblasts to lattice-shaped dermal fibroblasts. Our findings contribute to understanding the fundamental mechanisms of skin regeneration and have potential implications for developing new skin treatments.
DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Tosho Kondo, Ihori Ebinuma, Hirotaka Tanaka, Yukitoshi Nishikawa, Takaki Komiya, Mitsuru Ishikawa, Hideyuki Okano
Summary: In this study, an effective high-throughput screening system was established using induced pluripotent stem cells (iPSCs) to generate motor neurons. The generated motor neurons exhibited characteristics similar to ALS neurons and showed abnormal protein accumulation and hyperexcitability. Treatment with rapamycin and retigabine normalized activity and improved neuronal survival. This study provides a valuable platform for discovering novel ALS therapeutics and personalized medicine approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Shota Okura, Honoka Ishii, Ayano Suzuki, Chika Saegusa, Ko Fujiki, Kenshi Sugano, Noriomi Suzuki, Tsubasa Saeki, Saeko Matsuzaki, Hiroyuki Ozawa, Masato Fujioka, Makoto Hosoya, Hideyuki Okano
Summary: We have established two human-induced pluripotent stem cell (iPSC) lines from individuals without auditory disorders and confirmed normal hearing through extensive audiometry tests. These iPSC lines express pluripotency genes and are capable of differentiating into the three germ layers. They will be used as controls for pathological analysis and drug screening for ear disorders.
STEM CELL RESEARCH
(2023)
Article
Engineering, Biomedical
Hidenori Tani, Eiji Kobayashi, Shinomi Yagi, Keisuke Tanaka, Kotaro Kameda-Haga, Shinsuke Shibata, Nobuko Moritoki, Kaworu Takatsuna, Taijun Moriwaki, Otoya Sekine, Tomohiko C. Umei, Yuika Morita, Yusuke Soma, Yoshikazu Kishino, Hideaki Kanazawa, Jun Fujita, Shunji Hattori, Shugo Tohyama
Summary: This study found that heart-derived collagen is more effective in heart tissue engineering and can lead to more mature engineered heart tissue.
Article
Biochemistry & Molecular Biology
Hitomi Ono-Minagi, Tsutomu Nohno, Takashi Serizawa, Yu Usami, Takayoshi Sakai, Hideyuki Okano, Hideyo Ohuchi
Summary: This study investigates the embryonic origin of salivary and lacrimal glands using transgenic mice and demonstrates that these glands contain cells derived from all three germ layers. Specifically, a subset of cells derived from neural crest differentiate into epithelial cells and express high levels of stem cell markers. These cells play an important role in the regenerative response following tissue damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Clinical Neurology
Hiroya Kobayashi, Koji Ueda, Satoru Morimoto, Mitsuru Ishikawa, Nicolas Leventoux, Ryogen Sasaki, Yoshifumi Hirokawa, Yasumasa Kokubo, Hideyuki Okano
Summary: The proteins in extracellular vesicles derived from astrocytes in patients with Kii ALS/PDC exhibit distinctive patterns compared to healthy individuals, with increased proteins related to proteostasis and decreased proteins related to anti-inflammation.
NEUROLOGICAL SCIENCES
(2023)
Article
Obstetrics & Gynecology
Sohei Kuribayashi, Shinichiro Fukuhara, Go Tsujimura, Takahiro Imanaka, Koichi Okada, Norichika Ueda, Kentaro Takezawa, Hiroshi Kiuchi, Shigeyoshi Saito, Yusuke Takahashi, Hidetaka Kioka, Seiya Oura, Keisuke Shimada, Masahito Ikawa, Norio Nonomura
Summary: Chemical exchange saturation transfer (CEST) imaging can be used to assess testicular maturity and is important for evaluating the therapeutic effect of microscopic testicular sperm extraction.
REPRODUCTIVE MEDICINE AND BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Hideyuki Okano, Satoru Morimoto, Chris Kato, Jin Nakahara, Shinichi Takahashi
Summary: In recent years, clinical trials using iPSC technology for disease modeling and drug discovery have shown promising results, particularly for the treatment of ALS. ROPi has been identified as a potential ALS treatment and has undergone clinical trials and reverse translational research, demonstrating its ability to delay disease progression and prolong survival in ALS patients.
JOURNAL OF NEUROCHEMISTRY
(2023)