Journal
CURRENT OPINION IN CHEMICAL BIOLOGY
Volume 52, Issue -, Pages 79-84Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2019.05.020
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Funding
- Cross-ministerial Strategic Innovation Promotion Program (SIP)
- 'Technologies for creating next-generation agriculture, forestry and fisheries'
- Ministry of Education, Culture, Sports and Technology (MEXT) of Japan
- commission for Development of Artificial Gene Synthesis Technology for Creating Innovative Biomaterial from the Ministry of Economy, Trade and Industry (METI), Japan
- JSPS KAKENHI from JSPS, Japan [26119710, 16K14654]
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Technologies harnessing CRISPR systems have been rapidly evolving and expanding the capacity of researchers for understanding of mammalian cell behavior and its underlying mechanisms through genome and epigenome manipulations. In this review, we summarized the recent developments of CRISPR-based technologies for genetic and epigenetic modifications that include engineering of Cas9 for PAM simplification, non-cleaving base editing tools and alteration of gene expression. Applications such as genome-wide screening methods or CRISPR-based DNA barcoding for cellular lineage tracking are highlighted. Anticipated and upcoming development for mammalian synthetic biology that includes organelle engineering is also discussed.
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