Journal
BIOLOGICAL TRACE ELEMENT RESEARCH
Volume 177, Issue 1, Pages 10-15Publisher
HUMANA PRESS INC
DOI: 10.1007/s12011-016-0840-y
Keywords
Human endometrial stromal cells; In vitro decidualization; Oxidative stress; Copper
Funding
- National Science Foundation Project, China [31502131, 31402264]
- Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases [2013A061401013]
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The aim of this study was to investigate the effects of copper ions on decidualization of human endometrial stromal cells (HESCs) cultured in vitro. Firstly, non-toxic concentrations of copper D-gluconate were screened in HESCs based on cell activity. Then, the effects of non-toxic concentrations of copper ions (0 similar to 250 mu M) were examined on decidualization of human endometrial stromal cells. Our data demonstrated that the mRNA expressions of insulin-like growth factor binding protein (IGFBP-1), prolactin (PRL), Mn-SOD, and FOXO1were down-regulated during decidualization following the treatments with 100 or 250 mu M copper ions. Meanwhile, the amount of malonaldehyde (MDA) in the supernatant of HESCs was increased. These results showed that in vitro decidualization of HESCs was impaired by copper treatment.
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