Article
Chemistry, Analytical
Bang-Rui Li, Hao Tang, Ru-Qin Yu, Jian-Hui Jiang
Summary: A novel SP-ICP-MS assay for UDG activity detection has been developed with high sensitivity, demonstrating good applicability in real samples. This design may provide a new paradigm for developing enzyme activity detection in biological samples using SP-ICP-MS.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
I. Alvarez-Freire, T. Marques Rodriguez, A. M. Bermejo Barrera, P. Cabarcos-Fernandez, M. J. Tabernero-Duque
Summary: This study compared the methods for determination of levetiracetam in different laboratories and validated the reliability and linearity of the GC/MS method. Finally, the concentrations obtained by both methods were compared, showing a good correlation between the results.
MICROCHEMICAL JOURNAL
(2021)
Article
Medical Laboratory Technology
Carina Ankarberg-Lindgren, Charlotte Becker, Emilia Svala, Henrik Ryberg
Summary: In laboratory medicine, external quality assessment (EQA) schemes are versatile tools for detecting analytical flaws, but there is a lack of EQA schemes for pediatric sex steroid levels. This study aimed to compare different estradiol and testosterone immunoassays with clinical liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays in a pediatric setting. The results suggest that mass spectrometry based methods are recommended for the safe diagnosis and determination of sex steroids in children.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
David J. Handelsman, Graham Jones, Dorothy Kouzios, Reena Desai
Summary: In clinical practice, the accuracy of commercial direct steroid immunoassays is limited by cross-reactivity and nonspecific matrix interference, as well as the accuracy of manufacturer supplied calibrators. This study investigated the accuracy of calibrators for testosterone, estradiol, and progesterone immunoassays supplied by four manufacturers using UPLC-MS. The results showed significant deviations and potential inaccuracies in the manufacturer's supplied standards.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Endocrinology & Metabolism
Lauren R. Cirrincione, Bridgit O. Crews, Jane A. Dickerson, Matthew D. Krasowski, Jessica Rongitsch, Katherine L. Imborek, Zil Goldstein, Dina N. Greene
Summary: This analysis evaluates the bias in measuring estradiol levels in transgender women using three immunoassays and LC-MS/MS, and finds that some of the immunoassays may lead to falsely decreased concentrations.
ENDOCRINE CONNECTIONS
(2022)
Article
Food Science & Technology
Rongqi Zhai, Ge Chen, Guangyang Liu, Xiaodong Huang, Xiaomin Xu, Lingyun Li, Yanguo Zhang, Donghui Xu, A. M. Abd El-Aty
Summary: To detect imidacloprid residues in vegetables, colorimetric enzyme-linked immunoassay assay (Co-ELISA) and chemiluminescence enzyme-linked immunoassay assay (Cl-ELISA) were established. Cl-ELISA showed higher sensitivity, faster detection time, lower cost, and higher efficiency compared to Co-ELISA.
Article
Chemistry, Multidisciplinary
Zili Huang, Xiaobo Xie, Bei Xu, Rui Liu, Jianyu Hu, Yi Lv
Summary: In this study, a standard-free single magnetic bead nanoplatform using metal nanoparticles was proposed for prostate disease diagnosis. The inaccuracy of detection was reduced by evaluating metal intensities. The proportion of free PSA in total PSA was successfully evaluated using the Pt/Au intensity ratio, showing better differentiation between different disease states.
Review
Biochemical Research Methods
Shuting Xu, Huwei Liu, Yu Bai
Summary: Immunoassay is a crucial clinical technique for disease diagnosis. Mass spectrometry immunoassays (MSIAs) have become popular due to their powerful readout capabilities and improved sensitivity through the development of mass labels. MSIAs have expanded from element-based methods to laser-based and ambient MS, offering high potential for clinical applications such as biomarker screening and single-cell analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Ke Sherry Li, John G. Quinn, Matthew J. Saabye, Jesus F. Salcido Guerrero, Jim Nonomiya, Qihui Lian, Wilson Phung, Yevgeniy Izrayelit, Benjamin T. Walters, Amy Gustafson, Nicholas F. Endres, Maureen H. Beresini, Melinda M. Mulvihill
Summary: With recent advances in drug discovery, targeted covalent inhibitors have gained attention for their potential in treating acute and chronic diseases. This study presents a mass spectrometry-based platform for rapid kinetic analysis of irreversible covalent inhibitors. The platform allows for high-throughput characterization of covalent inhibitors through automated sample preparation and rapid MS analysis. A bimolecular reaction model is applied to extract important data, providing flexibility in studying covalent inhibitors with different properties.
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Michal Kaleta, Jana Oklestkova, Ondrej Novak, Miroslav Strnad
Summary: Neuroactive steroids are a family of compounds that can affect nervous system functions, synthesized in either the nervous system or peripheral endocrine tissues. Monitoring the production and levels of neuroactive steroids can be beneficial for the prevention, diagnosis, monitoring, and treatment of neurodegenerative and psychiatric diseases. Immunoanalytical methods and chromatography combined with mass spectrometry are highlighted as relevant techniques for analyzing neuroactive steroids.
Article
Agriculture, Dairy & Animal Science
Jose Renato S. Passos, Denise D. Guerreiro, Kamila S. Otavio, Pedro C. dos Santos-Neto, Marcela Souza-Neves, Federico Cuadro, Richard Nunez-Olivera, Martina Crispo, Maria Julia B. Bezerra, Renato F. Silva, Laritza F. Lima, Jose Ricardo Figueiredo, Ivan C. Bustamante-Filho, Alejo Menchaca, Arlindo A. Moura
Summary: The present study characterized the major proteome of preimplantation ovine embryos grown in vitro. The analysis identified proteins that are involved in translation, protein transport, and stabilization. Functional cluster analysis revealed differences between the in vitro and in vivo proteomes of sheep embryos, indicating that in vitro derived blastocysts have distinct protein profiles compared to those produced in vivo.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Chemistry, Multidisciplinary
Xiaowei Song, Qingce Zang, Chao Li, Tianhao Zhou, Richard N. N. Zare
Summary: We developed an immunoassay-based desorption electrospray ionization mass spectrometry imaging (immuno-DESI-MSI) technique for visualizing functional macromolecules. Boronic acid mass tags (BMTs) were synthesized to label antibodies as MSI probes, which can be released from the antibody by cleaving the boronic ester bond under acidic conditions. The BMTs can work in both optical and MS imaging modes and can be readily identified due to the introduction of the boron element.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Immunology
Lisha Ma, Wensheng Wang, Lisha Li, Ying Chen, Binxuan Chen, Miaoli Shao, Yongjun Cheng, Renfang Zhou
Summary: This study evaluated a novel fully automated immunoturbidimetric assay for the detection of anti-CCP antibodies and compared it with the conventional ELISA method. The results showed that the four anti-CCP assays provided similar diagnostic information for rheumatoid arthritis (RA), with Qiangsheng's method being potentially more useful for large scale clinical screening due to its shorter testing time.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Chuqing Zhou, Zecong Fang, Cunyi Zhao, Xiyan Mai, Shiva Emami, Ameer Y. Taha, Gang Sun, Tingrui Pan
Summary: This paper introduces a fully automated Robotic ELISA platform, which utilizes a robotic-microfluidic interface and a highly sensitive nanofibrous membrane to achieve sample-to-answer ELISA tests without human intervention. The platform can replace multiple manual steps involved in traditional ELISA operations, enhance efficiency, and achieve faster detection speed and higher sensitivity.
ANALYTICAL CHEMISTRY
(2021)
Review
Endocrinology & Metabolism
Jacquelien J. Hillebrand, Wjera Wickenhagen, Annemieke C. Heijboer
Summary: Despite little attention paid to hormone analyses in research studies, it is crucial to consider the quality of hormone measurements to prevent false conclusions and ensure appropriate follow-up studies. Various factors such as technique quality, expertise, matrices, timing and storage conditions, freeze-thaw cycles, variation, and standardization should be considered when conducting scientific research involving hormone measurements. This article aims to clarify the importance of paying attention to hormone analyses and provide guidance for decision-making in research studies.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Article
Medical Laboratory Technology
Jiansheng Lin, Weihua Lin, Yiming Lin, Weilin Peng, Zhenzhu Zheng
Summary: This study retrospectively analyzed the cases of Chinese infants with NICCD and identified multiple genetic mutations. The study also found comorbidity of NICCD and other inborn errors of metabolism in some patients.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Lihua Guan, Wei Su, Jian Zhong, Ling Qiu
Summary: Multiple myeloma is characterized by excessive production of monoclonal immunoglobulins. Routine screening methods are insufficient for detecting low levels of M proteins, but advances in mass spectrometry enable reliable detection of low abundance serum biomarkers for minimal residual disease assessment in multiple myeloma.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Liya Zhu, Chao Zhu, Jialie Jin, Jinxin Wang, Xiaojing Zhao, Rongxi Yang
Summary: This study found an association between blood-based ITGB2 methylation and coronary heart disease (CHD), with hypomethylation of ITGB2 being a risk factor for CHD. Additionally, the combination of ITGB2 methylation and conventional CHD risk factors could efficiently discriminate CHD patients from controls.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
H. Al Habobe, E. B. Haverkort, K. Nazmi, A. P. Van Splunter, R. H. H. Pieters, F. J. Bikker
Summary: Saliva diagnostics have become popular due to their non-invasive nature and patient-friendly collection process. However, the choice of saliva collection method can affect the measured levels of various biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Abdurrahman Coskun, Anna Carobene, Ozlem Demirelce, Michele Mussap, Federica Braga, Ebru Sezer, Aasne Karine Aarsand, Sverre Sandberg, Pilar Fernandez Calle, Jorge Diaz-Garzon, Metincan Erkaya, Cihan Coskun, Esila Nur Erol, Hunkar Dag, Bill Bartlett, Mustafa Serteser, Niels Jonker, Ibrahim Unsal
Summary: In this study, BV estimates for 22 AAs were provided based on a large sample size, and it was found that there are differences in CVI estimates between males and females for most AAs, which has implications for the clinical interpretation and use of AAs.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Valentinus Besin, Farizky Martriano Humardani, Trilis Yulianti, Matthew Justyn
Summary: The study of Parkinson's Disease in Asian populations has revealed the impact of genetic variants on multiple biological pathways and highlighted shared genetic susceptibility with other diseases. These findings emphasize the importance of personalized treatment based on individual genetic profiles.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Simona Ferraro, Sara Benedetti, Savina Mannarino, Santica Marcovina, Elia Mario Biganzoli, Gianvincenzo Zuccotti
Summary: Risk stratification for cardio-vascular disease should be implemented in childhood to promote early prevention strategies, as atherosclerotic lesions can be present even in very young individuals. Evaluating pediatric CV risk factors/clinical conditions and conducting lipid profile and genetic testing can help identify children at risk of future CV events and guide appropriate therapeutic options.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Ahmad Mobed, Bita Abdi, Sajjad Masoumi, Mohammad Mikaeili, Elham Shaterian, Hamed Shaterian, Esmat Sadat Kazemi, Mahdiye Shirafkan
Summary: Reproductive biomarkers play important regulatory roles in women. The discovery and quantification of these biomarkers are clinically significant. Various detection strategies, including nanotechnology-based methods, have been developed. This article provides an in-depth introduction to the latest advances in biosensor and nanosensor research for detecting and quantitatively identifying reproductive biomarkers.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Chengfang Tang, Fang Tang, Yanna Cai, Minyi Tan, Sichi Liu, Ting Xie, Xiang Jiang, Yonglan Huang
Summary: This study proposes an effective method for screening X-ALD and evaluates the performance of newborn screening for X-ALD in Guangzhou. The LC-MS/MS method can accurately identify X-ALD through analysis of C26:0-LPC.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Lan Liu, Jiamin Wang, Xijing Liu, Jing Wang, Lin Chen, Hongmei Zhu, Jingqun Mai, Ting Hu, Shanling Liu
Summary: The 16p11.2 deletion is a common genetic cause of neurodevelopmental disorders, with prenatal and postnatal presentations including vertebral malformations and language impairment. The majority of deletions are de novo and may be associated with MAPK3 and histidine-associated metabolism.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Sneha Venkatesalu, Shanmugapriya Dilliyappan, Avanthika Satish Kumar, Thirunavukkarasu Palaniyandi, Gomathy Baskar, Maddaly Ravi, Asha Sivaji
Summary: Microfluidics is a science and technology that deals with less sample-to-more precision in vitro analysis. It has wide applications in cancer theranostics, enabling precise diagnosis and personalized treatment.
CLINICA CHIMICA ACTA
(2024)
Review
Medical Laboratory Technology
Yuanqin Zhao, Wei Deng, Zhaoyue Wang, Yanxia Wang, Hongyu Zheng, Kun Zhou, Qian Xu, Le Bai, Huiting Liu, Zhong Ren, Zhisheng Jiang
Summary: The cardiovascular system and the central nervous system exhibit a coordinated developmental process during embryonic development. Congenital heart disease (CHD) is the most common congenital disorder, and neurodevelopmental disorders (NDD) are common complications in CHD patients. Both genetic and non-genetic factors contribute to the co-occurrence of CHD and NDD. Further research should focus on identifying common molecular mechanisms underlying this co-occurrence and promoting the research and treatment of developmental disorders related to the cardiovascular and central nervous systems.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Karol Gostomczyk, Ewelina Lukaszewska, Jedrzej Borowczak, Anita Bator, Marek Zdrenka, Magdalena Bodnar, Lukasz Szylberg
Summary: Flow cytometry improves the detection of epithelial cancer cells in peritoneal and pleural fluids compared to conventional cytology. Due to similar speciflcity and higher sensitivity, flow cytometry offers a promising alternative to cytology for patient screening.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Heping Tian, Genghuan Wang, Qi Zhong, Haihang Zhou
Summary: This study found that the decline of serum ITIH4 concentrations during the early phase after aneurysmal subarachnoid hemorrhage (aSAH) was closely related to the severity and poor prognosis of the disease. Serum ITIH4 may represent a promising prognostic biomarker of aSAH.
CLINICA CHIMICA ACTA
(2024)
Article
Medical Laboratory Technology
Xueting Zhu, Yang Yu, Jun Zhang, Yuxia Zhan, Guanghua Luo, Lu Zheng
Summary: This study successfully established a 2D-PCR method for identifying HLA-B*15:02 through a two-tube reaction. This method can distinguish HLA-B*15:02 from 16 highly homologous HLA-B*15 alleles. Among 1830 samples from the clinical general population, 3 HLA-B*15:02 homozygotes and 84 HLA-B*15:02 heterozygotes were detected.
CLINICA CHIMICA ACTA
(2024)
