Journal
CHEMBIOCHEM
Volume 21, Issue 6, Pages 797-800Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201900546
Keywords
berberine bridge enzyme; Citrus sinensis; enzyme catalysis; green chemistry; uronic acid oxidase
Funding
- Agency for Science, Research and Technology of Singapore Visiting Investigator Program [1535j00137]
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Uronic acid-rich plant materials such as pectin are potential renewable feedstocks for the chemical industry. Uronic acid oxidase activity was first reported in extracts of citrus leaves, and was subsequently found to be widely distributed in plants, with the highest activity detected in extracts of the pectin-rich citrus peel. Herein we report the identification of the primary sequence of uronic acid oxidase from extracts of the peel of Citrus sinensis, by partial purification and protein mass spectrometry. Activity of the enzyme, a member of the berberine bridge family, was confirmed by recombinant expression in Pichia pastoris. Biochemical characterization of the recombinant enzyme is reported. Our findings facilitate further investigation of the biological function of uronic acid oxidation in the economically important orange fruit, and also provide a basis for the development of a catalyst for bioconversion of uronic acids.
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