Review
Biochemistry & Molecular Biology
Anatoly F. Vanin
Summary: This article discusses the oxidative mechanism of M-DNICs formation and the effect of thiol-containing ligands on the stability of these complexes. The results demonstrate that thiol-containing ligands help M-DNICs maintain stability at neutral pH values.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Anatoly F. Vanin
Summary: This study proposed a mechanism for the formation of dinitrosyl iron complex (DNIC) and explored its biological activity. The results showed that the release of NO molecules and nitrosonium cations from these complexes had positive and negative effects, respectively. Additionally, it was suggested to enhance the selective release of nitrosonium cations and incorporate the released NO molecules into biologically non-active mononitrosyl iron complexes using dithiocarbamate derivatives.
BIOCHEMISTRY-MOSCOW
(2022)
Article
Chemistry, Multidisciplinary
Wenjie Tao, Curtis E. Moore, Shiyu Zhang
Summary: A redox-neutral S-nitrosation of thiol has been achieved at a dicopper(I,I) center, providing new insights into the mechanism of S-nitrosation. The reaction yielded thiol and di-S-nitrosothiol complexes, indicating that S-nitrosation proceeds through similar intermediate species.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Anatoly F. Vanin, Viktor A. Tronov, Rostislav R. Borodulin
Summary: This study demonstrates that binuclear dinitrosyl iron complexes with thiol-containing ligands exhibit cytotoxic effects on MCF7 human breast cancer cells, mediated by nitrosonium cations released from these complexes. The cytotoxic effects are retained or even enhanced in the presence of N-Methyl-D-glucamine dithiocarbamate, supporting the release of NO+ cations from a [Fe(NO)(2)] fragment.
CELL BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Tiffany M. Russell, Des R. Richardson
Summary: Glutathione-S-transferases (GSTs) are isozymes that play important roles in both detoxification and redox stress suppression by conjugating glutathione (GSH) to xenobiotics and interacting with cytotoxic nitric oxide (NO). The interactions between NO and GSTs involve binding and storage of NO as dinitrosyl-dithiol iron complexes (DNICs). GSTP1 can inhibit inducible nitric oxide synthase (iNOS) and suppress c-Jun N-terminal kinase (JNK) and nuclear factor kappa B (NF-kappa B) pathways, leading to decreased apoptosis and modulating NO-mediated ROS generation. These studies highlight the innovative role of GSTs in NO metabolism and their interactions with multiple effector proteins.
Article
Biochemistry & Molecular Biology
Jill B. Harland, Subhra Samanta, Nicolai Lehnert
Summary: In this study, three new synthetic model systems for NorBC were designed and characterized using spectroscopy. It was found that replacing the carboxylate ligand with a phenolate or pyridine group restored the reactivity towards NO, resulting in the formation of diiron dinitrosyl complexes. However, no N2O production was detected.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Wen-Han Chuang, Yu-Ting Chou, Yi-Hong Chen, Ting-Han Kuo, Wen-Feng Liaw, Tsai-Te Lu, Chih-Fei Kao, Yun-Ming Wang
Summary: Alzheimer's disease (AD) is a neurodegenerative disorder characterized by cognitive impairment, memory loss, and behavioral deficits. The aggregation of β-amyloid(1-42) (Aβ(1-42)) is a significant cause of AD pathogenesis. Despite extensive research, current treatment efficacy for AD remains insufficient. Nitric oxide (NO) has a neuroprotective role in the central nervous system by inhibiting Aβ aggregation and rescuing memory and learning deficits through the NO signaling pathway. Targeting the NO pathway may be a therapeutic option, but the limited half-life of NO in the biological system poses a challenge. This study explored a biomimetic dinitrosyl iron complex (DNIC-COOH) that can stably deliver NO to address this issue. Adding DNIC-COOH to neuron-like cells and primary cortical neurons along with Aβ(1-42) demonstrated its ability to protect neuronal cells, enhance neuronal functions, and facilitate Aβ(1-42) degradation through the NO-sGC-cGMP-AKT-GSK3β-CREB/MMP-9 pathway.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Chemistry, Inorganic & Nuclear
Chuan-Kuei Chiang, Yu-Chiao Liu, Kai-Ti Chu, Jing-Ting Chen, Cheng-Yeh Tsai, Gene-Hsiang Lee, Ming-Hsi Chiang, Chien-Ming Lee
Summary: In this study, a dimeric and mononuclear Fe-NO model complex were synthesized and characterized, showing the formation of a thiolate-bridged bimetallic complex during the reduction of the mononuclear complex. The electronic structure of the bimetallic complex was described as a FeII/{Fe(NO)2}9 state, providing insights into the NO scrambling behavior in the activity of flavodiiron nitric oxide reductases (FNORs).
INORGANIC CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Tatiana Lapina, Vladislav Statinov, Roman Puzanskiy, Elena Ermilova
Summary: The study demonstrates that colorless alga Polytomella parva cells synthesize nitric oxide (NO) through L-arginine and proposes the existence of a nitric oxide synthase-like activity independent of nitrate reductase (NR). The NO generated causes S-nitrosation of protein cysteine thiol groups as an essential post-translational modification in P. parva.
Article
Biochemistry & Molecular Biology
Alexandra Igrunkova, Alexey Fayzullin, Natalia Serejnikova, Tatiana Lipina, Alexandr Pekshev, Anatoly Vanin, Victoria Zaborova, Elena Budanova, Dmitry Shestakov, Igor Kastyro, Anatoly Shekhter
Summary: This study compared the wound healing effects of B-DNIC-GSH and NO-CGF. The results showed that B-DNIC-GSH had a more pronounced effect in reducing inflammation and promoting wound healing compared to NO-CGF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Christopher H. Switzer, Hyun-Ju Cho, Thomas R. Eykyn, Paul Lavender, Philip Eaton
Summary: Inducible nitric oxide synthase (NOS2) and nitric oxide (NO) signaling lead to DNA hypomethylation and genomic instability through degradation of DNMT1 protein and retrotransposon activation. NOS2 expression levels are correlated with decreased DNA methylation and malignant cellular transformation in breast cancer patients.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Inorganic & Nuclear
Wen-Chieh Chang, Wan-Tin Du, Yi-Xuan Lin, Ruei-Lin Jhang, Chung-Hung Hsieh
Summary: This article describes the synthesis and characterization of cationic, neutral, and anionic DNCCs, demonstrating the interconversions and NO releasing ability akin to what is observed in DNICs.
DALTON TRANSACTIONS
(2023)
Article
Chemistry, Inorganic & Nuclear
Wen-Chieh Chang, Wan-Tin Du, Yi-Xuan Lin, Ruei-Lin Jhang, Chung-Hung Hsieh
Summary: This article describes the synthesis and characterization of cationic, neutral, and anionic DNCCs, demonstrating the interconversions and NO releasing ability akin to what is observed in DNICs.
DALTON TRANSACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Yu-Chieh Chen, Yi-Hong Chen, Han Chiu, Yi-Hsuan Ko, Ruei-Ting Wang, Wei-Ping Wang, Yung-Jen Chuang, Chieh-Cheng Huang, Tsai-Te Lu
Summary: The study investigated the cellular uptake, intracellular release, and regulatory effects of a compound DNIC-2 on epidermal cells. The results showed that DNIC-2 can be taken up by human fibroblast cells, transformed into protein-bound forms, and release NO intracellularly, leading to enhanced cellular proliferation, accelerated wound healing, and increased collagen deposition. The biocompatible DNIC-2 holds potential to be a novel active ingredient for skincare products based on in vitro and in vivo biocompatibility evaluation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Inorganic & Nuclear
Tiankun Zhao, Yong Zhang, Peng Wang, Shanjia Li, Zhongduo Yang, Mingjun Yang
Summary: Three dinitrosyl cobalt complexes (DNCCs) were synthesized from CoCl2 center dot 6H(2)O, with final yields ranging from 53% to 67%, and isolated via fast column chromatography. These EPR silent DNCCs showed only one pair of redox events in the cyclic voltammograms. Compared to sodium nitroprusside (SNP), the DNCCs exhibited significantly enhanced NO release amount and duration, which were closely related to their hydrolytic stability. Additionally, the DNCCs showed higher NO cellular uptake rates in the first 5 hours than SNP by HUVEC, and had low cytotoxicity. A plausible NO release mechanism via a one electron reduction process was proposed based on the hydrolysis of DNCCs and the possible formation of [(Dppp)Co(NO)(2)] and [(Dppp)Co(PPh3)(NO)] in the reduction of [(Dppp)Co(NO)(2)]Cl.
INORGANIC CHEMISTRY COMMUNICATIONS
(2023)