Article
Mechanics
Takayuki Suzuki, Srivathsan Kalyan, Cynthia Berlinicke, Samantha Yoseph, Donald J. Zack, Soojung Claire Hur
Summary: Viscoelastic focusing is a promising method for manipulating micro and nanoscale bioparticles. This study investigates the mechanisms and applications of viscoelastic focusing in rectangular microchannels. By varying the fluid elasticity and inertia, the flow characteristics driving cell focusing are revealed. The findings provide guidance for the application of viscoelastic focusing in flow cytometry and particle sorting, as well as valuable insights for device design.
Article
Polymer Science
Meng Zhang, Zihuang Wang, Yanhua Zheng, Bifeng Zhu, Bingzhi Zhang, Xiaohui Fang, Wenli Shang, Wu Zhang
Summary: This paper reports on the manipulation of elastic instability of a viscoelastic fluid in a rhombus cross microchannel structure. The study demonstrates bistable and unsteady instabilities of flow in a standard cross microchannel for reference. It is found that the stabilizing effect of the rhombus structure enables the maintenance of bistable instability over a wider range of injection rates in the rhombus cross microchannel. Additionally, a semi-bistable instability is established at high injection rates. Moreover, unsteady elastic instability is achieved in the rhombus cross microchannel through asymmetric injection rate conditions.
Article
Chemistry, Analytical
Yuchen Dai, Haotian Cha, Michael J. Simmonds, Hedieh Fallahi, Hongjie An, Hang T. Ta, Nam-Trung Nguyen, Jun Zhang, Antony P. McNamee
Summary: This study investigates the effects of altering fluid viscoelasticity on blood plasma extraction. Poly (ethylene oxide) (PEO) was added to increase blood viscoelasticity, resulting in improved cell focusing performance. The optimal PEO concentration was found to be in the range of 100 to 200 ppm, and the optimal flow rate was determined to be between 1 and 15 mu L/min.
Article
Chemistry, Analytical
Yuwen Lu, Wei Tan, Xin Shi, Mingwei Liu, Guorui Zhu
Summary: The research demonstrates a size-based particle manipulation method using the Viscoelastic Stagnant Region (VSR) to capture sensitive bioparticles, showing size selectivity for 20μm and 5μm polystyrene particles. The particles manipulated by VSR exhibit low velocity motion with spiral trajectories, indicating a different behavior compared to inertial vortices. The activity of Crypthecodinium cohnii (C. cohnii) was slightly decreased by VSR processing, but completely lost after being processed by inertial vortices, highlighting the potential of VSR in preserving the activity of vulnerable biological cells.
Article
Chemistry, Analytical
Yao Chen, Lin Jiang, Xiaozhe Zhang, Zhonghua Ni, Nan Xiang
Summary: This article introduces an efficient label-free sorting and mechanical phenotyping method for tumor cells, which utilizes a viscoelastic-sorting integrated deformability cytometer (VSDC) to sort, extract, and analyze the mechanical properties of tumor cells. Machine learning algorithms are used to distinguish tumor cells from white blood cells.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Malihe Farasat, Ehsan Aalaei, Saeed Kheirati Ronizi, Atin Bakhshi, Shaghayegh Mirhosseini, Jun Zhang, Nam-Trung Nguyen, Navid Kashaninejad
Summary: Separation and detection of cells and particles in a suspension are essential for various applications, including biomedical investigations and clinical diagnostics. Microfluidics has enabled the miniaturization of analytical devices by controlling the motion of fluid in micrometer-sized channels and chambers. Among different microfluidic methods, dielectrophoresis (DEP) offers advantages for manipulating neutral bioparticles using unique dielectric characteristics. This review focuses on signal-based DEP methods that utilize signal parameters such as frequency, amplitude, phase, and shape for cell/particle separation and manipulation.
Article
Chemistry, Analytical
Arash Dalili, Hossein Montazerian, Kabilan Sakthivel, Nishat Tasnim, Mina Hoorfar
Summary: This study investigated the factors affecting the displacement of particles in electrophoresis-based microseparators, finding that volumetric throughput has the greatest impact on particle position, with channel length being the most effective factor. The study also successfully demonstrated size-selective sorting or focusing of particles, providing valuable insights into designing microfluidic platforms for selectively separating different cell types in biofluids.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Engineering, Chemical
Ioannis H. Karampelas, Jenifer Gomez-Pastora
Summary: The demand for precise separation of particles, cells, and other biological matter has increased in recent years, leading to increased scientific interest in the topic. This article establishes the theoretical basis for fluid flow and particle separation and summarizes computational work done in the field of particle and cell separation, with a focus on magnetic, dielectric, and acoustic methods.
Article
Mechanics
Yunxing Su, Alfonso Castillo, On Shun Pak, Lailai Zhu, Roberto Zenit
Summary: In this study, it is found that due to the asymmetric shear-induced elastic stresses, a dense sphere rotating near a wall can levitate at a fixed distance away from the wall by generating a net elastic vertical force.
JOURNAL OF FLUID MECHANICS
(2022)
Article
Biochemical Research Methods
Dan Yuan, Sharda Yadav, Hang T. Ta, Hedieh Fallahi, Hongjie An, Navid Kashaninejad, Chin Hong Ooi, Nam-Trung Nguyen, Jun Zhang
Summary: This study investigated sheathless viscoelastic focusing of particles and cells in a zigzag microfluidic channel, demonstrating its application on various types of particles and cells. The zigzag structure of the channel allows for reduced device footprint and channel length without sacrificing focusing performance.
Article
Engineering, Chemical
Bimalendu Mahapatra, Aditya Bandopadhyay
Summary: Geometrically modified microchannels fabricated using stereolithography are used to study micromixing of polymeric solutions. Experimental and numerical analyses were conducted to evaluate the validity of Newtonian fluid flow in these channels. The presence of inertio-elastic instability in the geometrically modified microchannels for viscoelastic fluid transport is observed, which significantly enhances the mixing efficiency. This analysis provides important insights for the cost-effective design and operation of microfluidic devices handling viscoelastic fluids, particularly in designing passive micromixers for bio/polymeric fluids transportation.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Chemistry, Analytical
Yuwei Lan, Yang Zhou, Man Wu, Chunping Jia, Jianlong Zhao
Summary: The isolation of single cells or droplets is a crucial step in single-cell analysis, particularly important for cancer research and diagnostic methods. This review provides an overview of current and developing technologies for achieving this isolation. Microfluidic-based manipulation, with its advantages of miniaturization and low cost, has gained extensive attention recently. Five microfluidic strategies including trapping, discrete manipulation, bioprinting, capillary, and inertial force, are discussed in terms of their principles, features, modified approaches, applications, advantages, drawbacks, and future trends.
Review
Chemistry, Multidisciplinary
Aliakbar Ebrahimi, Kutay Icoz, Reza Didarian, Chih-Hsin Shih, E. Alperay Tarim, Behzad Nasseri, Ali Akpek, Berivan Cecen, Ayca Bal-Ozturk, Kadri Gulec, Yi-Chen Ethan Li, Steven Shih, Burcu Sirma Tarim, H. Cumhur Tekin, Emine Alarcin, Mehdi Tayybi-Azar, Hamed Ghorbanpoor, Ceren Ozel, Ayla Eker Sariboyaci, Fatma Dogan Guzel, Nicole Bassous, Su Ryon Shin, Huseyin Avci
Summary: This review comprehensively discusses lab-on-a-chip methods for the separation of biomolecules developed in the past decade, including passive, active, and hybrid approaches. It highlights the investigation of combined sophisticated methods and explores how separation successes permit the development of novel techniques.
ADVANCED MATERIALS INTERFACES
(2023)
Article
Chemistry, Analytical
Navya Rastogi, Pranjal Seth, Ramray Bhat, Prosenjit Sen
Summary: The novel orthogonal vortex chip enables size-based cell separation at lower velocities to avoid cell damage caused by high flow speeds. The device utilizes shear-gradient lift, centrifugal, and Dean flow drag forces to selectively trap particles of different sizes, showing promising results for gentle isolation of rare cells from blood.
ANALYTICA CHIMICA ACTA
(2021)
Article
Mechanics
Gaofeng Chen, Xikai Jiang
Summary: We numerically studied the dynamics of a single particle suspended in a low-Reynolds-number fluid under spherical confinement. The hydrodynamic mobilities of different particle shapes and orientations were calculated parallel and transverse to the particle-cavity line of centres. The mobilities decreased as the particle moved towards the no-slip wall. Anisotropy played a role in the mobility, which became stronger with increased confinement level. The findings have implications for intracellular transport and microfluidic applications.
JOURNAL OF FLUID MECHANICS
(2023)
Review
Engineering, Electrical & Electronic
Mohammad K. D. Manshadi, Mehdi Mohammadi, Mohammad Zarei, Mahsa Saadat, Amir Sanati-Nezhad
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2020)
Letter
Virology
Sedigheh Basirjafari, Masoumeh Rafiee, Babak Shahhosseini, Mehdi Mohammadi, Saeideh Aghayari Sheikh Neshin, Mohammad Zarei
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Masoud Nouri-Vaskeh, Neda Khalili, Amirreza Khalaji, Pouya Behnam, Leila Alizadeh, Sara Ebrahimi, Neda Gilani, Mehdi Mohammadi, Seyed Alisalar Madinehzadeh, Mohammad Zarei
Summary: This study found that circulating levels of GLP-1 were lower in patients with liver cirrhosis compared to healthy controls, and were associated with the severity of liver disease.
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Editorial Material
Infectious Diseases
Mohammad Zarei, Deepanwita Bose, Soheila Ali Akbari Ghavimi, Masoud Nouri-Vaskeh, Mehdi Mohammadi, Amirhossein Sahebkar
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2021)
Review
Virology
Maryamsadat Jafari, Ali Pormohammad, Saeideh Aghayari Sheikh Neshin, Saied Ghorbani, Deepanwita Bose, Shohreh Alimohammadi, Sedigheh Basirjafari, Mehdi Mohammadi, Cody Rasmussen-Ivey, Mohammad Hossein Razizadeh, Masoud Nouri-Vaskeh, Mohammad Zarei
Summary: Pregnant women infected with COVID-19 exhibit similar symptoms to non-pregnant adult patients, with a higher likelihood of fever and fatigue and a lower likelihood of cough, headache, and diarrhea. Ground-glass opacity is the most common imaging finding in pregnant women, while consolidation is more common in non-pregnant patients. Pregnant patients have a lower risk of vertical transmission.
REVIEWS IN MEDICAL VIROLOGY
(2021)
Review
Chemistry, Analytical
Asmaa Khater, Osama Abdelrehim, Mehdi Mohammadi, Abdulmajeed Mohamad, Amir Sanati-Nezhad
Summary: Droplet microfluidic platforms have made significant progress in chemical, biological, and medical applications by controlling droplet temperature and enabling multi-functional droplet operations. They can be applied in various medical, chemical, and engineering fields through precise manipulation and offer new methods to meet different demands.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Review
Immunology
Ali Pormohammad, Mohammad Zarei, Saied Ghorbani, Mehdi Mohammadi, Mohammad Hossein Razizadeh, Diana L. Turner, Raymond J. Turner
Summary: This study systematically reviewed the clinical features of COVID-19 vaccines in clinical trials, finding that mRNA-based and adenovirus-vectored vaccines exhibited different efficacy and side effect profiles. Both types of vaccines showed high efficacy after the first and second doses, with mRNA-based vaccines having higher reported side effects.
Article
Biophysics
Mehdi Mohammadi, Hossein Zargartalebi, Razieh Salahandish, Raied Aburashed, Kar Wey Yong, Amir Sanati-Nezhad
Summary: This study provides an overview of the role of exosomes in cancer diagnosis and prognosis, investigates emerging exosome technologies, evaluates the advantages and drawbacks of different technologies, and discusses their clinical challenges for cancer diagnosis and prognosis. Commercial platforms for exosomal detection and analysis are introduced, with a focus on their performance and impact on cancer diagnosis and prognosis, as well as the risks associated with the development of next-generation exosome devices.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Multidisciplinary Sciences
Yousef Alihosseini, Mohammad Reza Azaddel, Sahel Moslemi, Mehdi Mohammadi, Ali Pormohammad, Mohammad Zabetian Targhi, Mohammad Mahdi Heyhat
Summary: Research shows that the circular cross-section is the most efficient shape for the microchannel heat sink, with a 25% increase in heat transfer compared to the square shape at Reynolds number of 1150. Additionally, placing the circular cross-section microchannel below the PCR device can cool saliva samples in 16.5 seconds, saving 157.5 seconds for the whole amplification procedure compared to conventional air fans. Another advantage of using the microchannel heat sink is its small space requirements compared to other common cooling methods.
SCIENTIFIC REPORTS
(2021)
Article
Engineering, Electrical & Electronic
Farideh Salimian Rizi, Shahram Talebi, Mehdi Mohammadi
Summary: This paper proposes a design framework for separating particles such as red blood cells and exosomes on a single chip, using a low voltage direct current electric field to generate vortices for particle trapping. The results demonstrate that the generated vortices are strong enough to trap both micro and nanoparticles, with the chip having advantages of low voltage requirement and easy manufacture.
INTERNATIONAL JOURNAL OF NUMERICAL MODELLING-ELECTRONIC NETWORKS DEVICES AND FIELDS
(2022)
Review
Immunology
Ali Pormohammad, Mohammad Zarei, Saied Ghorbani, Mehdi Mohammadi, Saeideh Aghayari Sheikh Neshin, Alireza Khatami, Diana L. Turner, Shirin Djalalinia, Seied Asadollah Mousavi, Heydar Ali Mardani-Fard, Amir Kasaeian, Raymond J. Turner
Summary: This study supports the short-term effectiveness of vaccines, especially mRNA-based vaccines, in preventing Delta variant infection, hospitalization, severe infection, and death.
Article
Microbiology
Mehdi Mohammadi, Stephanie L. Bishop, Raied Aburashed, Saad Luqman, Ryan A. Groves, Dominique G. Bihan, Thomas Rydzak, Ian A. Lewis
Summary: Metabolomics is a popular approach in microbial metabolism research, and quantification of metabolic boundary fluxes is one of its emerging applications. By quantifying metabolic boundary fluxes, complex metabolic phenotypes can be rapidly captured, computational models can be developed, and it can be used in clinical diagnostics. Quantifying metabolic boundary fluxes requires minimal sample processing, which is advantageous compared to traditional intracellular metabolite level analysis.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Farideh Salimian Rizi, Shahram Talebi, Mohammad K. D. Manshadi, Mehdi Mohammadi
Summary: Bloodstream infections have a high mortality rate, and the inability to detect pathogens quickly is a major factor contributing to this. Finding an effective method for separating bacteria from the blood is crucial, and the insulator-based technique shows promise in increasing separation efficiency. By utilizing dielectrophoresis in a microchannel, different sizes of bacteria can be successfully sorted.
SEPARATION SCIENCE PLUS
(2023)
Article
Biophysics
Farideh Salimian Rizi, Shahram Talebi, Mohammad K. D. Manshadi, Mehdi Mohammadi
Summary: Bloodstream infection is a life-threatening condition causing a significant number of deaths and infections annually in North America. The current study proposes a bacterial enrichment and separation approach using insulator-based dielectrophoresis to accelerate the diagnosis of infection. The model demonstrates efficient separation of particles based on size, preventing the accumulation of larger particles in the channel.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2023)
Article
Microbiology
Ali Pormohammad, Andrea Firrincieli, Daniel A. Salazar-Aleman, Mehdi Mohammadi, Dave Hansen, Martina Cappelletti, Davide Zannoni, Mohammad Zarei, Raymond J. Turner
Summary: The constant, ever-increasing antibiotic resistance crisis requires urgent development of novel antibiotics according to the World Health Organization. Silver-tellurite combination treatment shows promising synergistic antibacterial activity, surpassing common antibiotics and reducing the risk of future resistance chance. The mechanism involves targeting cellular processes and amplifying changes. Adding tellurite improves the efficacy of silver in biomedical applications, providing alternative antimicrobial options with greater stability and lower toxicity.
MICROBIOLOGY SPECTRUM
(2023)
